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  4. Recombinant Woodchuck hepatitis B virus Protein X (X)

Recombinant Woodchuck hepatitis B virus Protein X (X)

In Stock
  • 货号：
    CSB-EP323774WAW
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    X
  • Uniprot No.：
  • 别名：
    X; Protein X; HBx; Peptide X; pX
  • 种属：
    Woodchuck hepatitis B virus (isolate 8) (WHV)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    19.2kDa
  • 表达区域：
    1-141aa
  • 氨基酸序列
    MAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAASSASSPSPSDESDLPLGRLPACFASASGPCCLVFTCADLRTMDSTVNFVSWHANRQLGMPSKDLWTPYIKDQLLTKWEEGSIDPRLSIFVLGGCRHKCMRLL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The process of expressing the recombinant Woodchuck hepatitis B virus Protein X (X) protein in the E.coli requires the recombinant DNA gene formed by the integration of encoding gene for the 1-141aa of the Woodchuck hepatitis B virus Protein X (X) protein and N-terminal 6xHis tag sequence, the expression vector that the recombinant DNA gene inserts into, the E.coli that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-tagged recombinant Protein X (X) protein was obtained. This recombinant Protein X (X) protein is characterized by high purity (>90%, SDS-PAGE). This Protein X (X) protein ran along the gel to the band of approximately 21 kDa molecular weight.

    Protein X (also known as peptide X) is a protein encoding by a gene named X in Woodchuck hepatitis B virus. Protein X has no counterparts in any of its hosts and is conserved among mammalian hepadnavirus. This protein is well known for its transactivation activity through interaction with several cellular transcription factors, In addition to its role in viral replication and chromosomal instability, it is also known as an oncogene involving in modulating oncogenic pathways, oxidative stress, DNA methylation, angiogenesis and migration.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multifunctional protein that plays a role in silencing host antiviral defenses and promoting viral transcription. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma). Most of cytosolic activities involve modulation of cytosolic calcium. The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted. May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential. May also modulate apoptosis by binding host CFLAR, a key regulator of the death-inducing signaling complex (DISC). Promotes viral transcription by using the host E3 ubiquitin ligase DDB1 to target the SMC5-SMC6 complex to proteasomal degradation. This host complex would otherwise bind to viral episomal DNA, and prevents its transcription. Moderately stimulates transcription of many different viral and cellular transcription elements. Promoters and enhancers stimulated by HBx contain DNA binding sites for NF-kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT.
  • 亚细胞定位：
    Host cytoplasm. Host nucleus. Host mitochondrion.
  • 蛋白家族：
    Orthohepadnavirus protein X family