Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Ubiquinone biosynthesis protein COQ7 homolog (clk-1)

Recombinant Ubiquinone biosynthesis protein COQ7 homolog (clk-1)

  • 货号：
    CSB-YP005806CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005806CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005806CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005806CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005806CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    clk-1
  • Uniprot No.：
  • 别名：
    clk-1; ZC395.2; 5-demethoxyubiquinone hydroxylase; mitochondrial; DMQ hydroxylase; EC 1.14.13.-; Clock abnormal protein 1; Protein clk-1; Ubiquinone biosynthesis monooxygenase COQ7
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-187
  • 氨基酸序列
    MFRVITRGAH TAASRQALIE KIIRVDHAGE LGADRIYAGQ LAVLQGSSVG SVIKKMWDEE KEHLDTMERL AAKHNVPHTV FSPVFSVAAY ALGVGSALLG KEGAMACTIA VEELIGQHYN DQLKELLADD PETHKELLKI LTRLRDEELH HHDTGVEHDG MKAPAYSALK WIIQTGCKGA IAIAEKI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides. Involved in lifespan determination in a ubiquinone-independent manner.
  • 基因功能参考文献：
    1. Loss of ubiquinone biosynthesis is responsible for all phenotypes resulting from loss of CLK-1, including behavioral phenotypes, altered expression of mitochondrial quality control genes, and lifespan. PMID: 28404998
    2. Nuclear CLK-1 mediates a retrograde signalling pathway that is conserved from Caenorhabditis elegans to humans and is responsive to mitochondrial reactive oxygen species, thus acting as a barometer of oxidative metabolism. PMID: 25961505
    3. no measurable intrinsic ETC defect exists in clk-1 mitochondria. The data indicate that DMQ(9) specifically inhibits electron transfer from complex I to ubiquinone. PMID: 21745495
    4. Complementation of Escherichia coli ubiF mutation by Caenorhabditis elegans CLK-1. PMID: 12753928
    5. Caenorhabditis elegans clk-1 mutants are sensitive to ubiquinone side-chain length PMID: 12893826
    6. in maternally resqued animals, the effect of clk-1 mutations on adult lifespan in uncoupled from their effects on development and reproduction PMID: 14517217
    7. differential fertility of clk-1 mutant nematodes fed Q isoforms may result from changes in Q localization, altered recognition by Q-binding proteins, and/or potential defects in mitochondrial function resulting from the mutant CLK-1 polypeptide itself. PMID: 14530273
    8. Suppressors of the missense mutation of clk-1 were identified; each mutant suppresses a different subset of phenotypes. PMID: 16648490
    9. action of clioquinol on several age-dependent neurodegenerative diseases with distinct etiologies might result from a slowing down of the aging process through action of the drug on CLK-1. PMID: 18927074

    显示更多

    收起更多

  • 亚细胞定位：
    Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
  • 蛋白家族：
    COQ7 family
  • 数据库链接：

    KEGG: cel:CELE_ZC395.2

    STRING: 6239.ZC395.2.2

    UniGene: Cel.10672