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  4. Recombinant Treponema pallidum 47 kDa membrane antigen (TP_0574)

Recombinant Treponema pallidum 47 kDa membrane antigen (TP_0574)

  • 中文名称：
    梅毒螺旋体TP_0574重组蛋白
  • 货号：
    CSB-YP339039TPR
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    梅毒螺旋体TP_0574重组蛋白
  • 货号：
    CSB-EP339039TPR
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    梅毒螺旋体TP_0574重组蛋白
  • 货号：
    CSB-EP339039TPR-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    梅毒螺旋体TP_0574重组蛋白
  • 货号：
    CSB-BP339039TPR
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    梅毒螺旋体TP_0574重组蛋白
  • 货号：
    CSB-MP339039TPR
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TP_0574
  • Uniprot No.：
  • 别名：
    TP_0574; Putative DD-carboxypeptidase TP_0574; EC 3.4.-.-; 47 kDa lipoprotein; Tp47; Tpp47; 47 kDa membrane antigen; 47-kilodalton major integral membrane immunogen
  • 种属：
    Treponema pallidum (strain Nichols)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-434
  • 氨基酸序列
    C GSSHHETHYG YATLSYADYW AGELGQSRDV LLAGNAEADR AGDLDAGMFD AVSRATHGHG AFRQQFQYAV EVLGEKVLSK QETEDSRGRK KWEYETDPSV TKMVRASASF QDLGEDGEIK FEAVEGAVAL ADRASSFMVD SEEYKITNVK VHGMKFVPVA VPHELKGIAK EKFHFVEDSR VTENTNGLKT MLTEDSFSAR KVSSMESPHD LVVDTVGTGY HSRFGSDAEA SVMLKRADGS ELSHREFIDY VMNFNTVRYD YYGDDASYTN LMASYGTKHS ADSWWKTGRV PRISCGINYG FDRFKGSGPG YYRLTLIANG YRDVVADVRF LPKYEGNIDI GLKGKVLTIG GADAETLMDA AVDVFADGQP KLVSDQAVSL GQNVLSADFT PGTEYTVEVR FKEFGSVRAK VVAQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus. Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates. May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete. Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic. Binds penicillin. Penicillin binding is covalent, does not require lipidation, and is zinc-dependent. While this protein has beta-lactamase activity in vitro, that is probably not its role in vivo, as T.pallidum is very sensitive to penicillin antibiotics.; A pathogen-specific membrane antigen. Most abundant of the membrane lipoproteins, only found in pathogenic treponemes, suggesting that it is an important structural moiety in the cell envelope of virulent treponemal subspecies. A lipopeptide corresponding to the first 6 mature residues induces host (human and mouse) cytokine release by monocyte cell lines via TLR2 and CD14; nonlipidated protein does not stimulate host cells. Stimulates host (human) dendritic cell maturation to become MHC class II-positive antigen presenting cells via TLR2, which depends on lipidation; nonlipidated protein does not stimulate maturation.
  • 亚细胞定位：
    Cell inner membrane; Lipid-anchor.
  • 数据库链接：

    KEGG: tpa:TP_0574

    STRING: 243276.TP0574