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  4. Recombinant Transcription factor elt-1 (elt-1)

Recombinant Transcription factor elt-1 (elt-1)

  • 货号：
    CSB-YP329821CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP329821CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP329821CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP329821CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP329821CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    elt-1
  • Uniprot No.：
  • 别名：
    elt-1; W09C2.1Transcription factor elt-1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-416
  • 氨基酸序列
    MDYEGKPVEF TLGTSSGGAS LAPTSSTTAA SIAPFSYNTS ATNYYNTTPS SYPMFLNYQY AGGTTVTTDM DAFSGMDMSM NNGVFGTQNN PSYFYPTTQL NTYGYDTLAA ATTASGITVN NNQLNVNIVQ GNGTIVPQPI TQNIISTVSN VQSSVPINNS QPLTPTGLAG CSTSSGSSSA SSSSANSTST PKNTISKANR SSGGANNSQF STEDRECVNC GVHNTPLWRR DGSGNYLCNA CGLYFKMNHH ARPLVKPKKR QQNAQKRTGI ECVNCRTNTT TLWRRNGEGH PVCNACGLYF KLHKVERPIT MKKDGIQTRN RKLSAKGSRR MKKENGGTPT SMGMPTTSSS ISSGIELDQS GVWGMKNTQP MLMTPTAYAF PASNFYFNSI EDQLEYKTCP PMMVDFGGQM KNLNLN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that binds to the consensus sequence 5'-[AT]GATA[AG]-3' and variations thereof. During embryonic development, required for specification of cell fate of major hypodermal (epidermal) cells at the blastomere stage. The requirement is true for all four lineages derived from ABarp, ABpra and C blastomeres. Required for seam cell maintenance in late embryogenesis, for proper formation of dauer larvae and locomotion. Regulates expression of bro-1, a regulator of seam cell proliferation, via a GATA-like binding motif. Probably represses expression of eff-1 to prevent fusion of seam cells with the hypodermal syncytium. Involved in regulating expression of elt-3, a probable downstream target in hypodermal development pathways, in dorsal and ventral hypodermal cells. During postembryonic development, has a role in sperm development. Specifically, binds the bipartite consensus sequence 5'-AGATCTx(8)GATAA-3' found in genes with sperm-specific expression. Furthermore, regulates timing of developmental steps, possibly in parallel with daf-12, by controlling the expression of the let-7 family of miRNAs which in turn determines the transcription of heterochronic genes such as lin-41.
  • 基因功能参考文献：
    1. this work suggest that the contribution of ELT-1 to the control of let-7 family microRNA expression is likely through direct transcription regulation. PMID: 25816370
    2. Data suggest that ELT-1 has dual roles in the stem-like seam cells, acting both to promote proliferation and prevent differentiation. PMID: 21829390
    3. elt-1 is a key regulator of neuronal function in larvae and adult worms. PMID: 16303852
    4. analysis of regulation of sperm gene expression by the GATA factor ELT-1 PMID: 19591818
  • 亚细胞定位：
    Nucleus. Perikaryon. Cell projection, axon.
  • 组织特异性：
    Expressed in ventral cord and retrovesicular ganglion neurons. Expressed in vulval muscles of hermaphrodites and lateral seam and tail seam cells of males. Expressed in spermatozoa within the spermatheca of hermaphrodites.
  • 数据库链接：

    STRING: 6239.W09C2.1a

    UniGene: Cel.7646