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  4. Recombinant Staphylococcus aureus Staphopain B (sspB)

Recombinant Staphylococcus aureus Staphopain B (sspB)

  • 货号：
    CSB-YP645167FLF
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP645167FLF
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP645167FLF-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP645167FLF
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP645167FLF
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sspB
  • Uniprot No.：
  • 别名：
    sspB; SAOUHSC_00987Staphopain B; EC 3.4.22.-; Staphylococcal cysteine proteinase B; Staphylopain B
  • 种属：
    Staphylococcus aureus (strain NCTC 8325)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    220-393
  • 氨基酸序列
    D QVQYENTLKN FKIREQQFDN SWCAGFSMAA LLNATKNTDT YNAHDIMRTL YPEVSEQDLP NCATFPNQMI EYGKSQGRDI HYQEGVPSYE QVDQLTKDNV GIMILAQSVS QNPNDPHLGH ALAVVGNAKI NDQEKLIYWN PWDTELSIQD ADSSLLHLSF NRDYNWYGSM IGY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Our lab is planning to purchase recombinant protease sspB (CSB-EP645167FLF), likely 0.5 or 1mg purified from E. coli. How long would the turnaround time for this order be? Also, have you tested the proteolytic activity of the purified protein? Thank you very much,

A:
Thank you for your message and your interest in Cusabio products. This is Eunice replying you.
We haven't tested the activity of this protein, cannot 100% guaranteed. What we provide is the full length of mature protein, and it is purified under mild condition. It is active in theory.
By the way, could you please tell you need this protein for research use or reselling use?

靶点详情

  • 功能：
    Cysteine protease that plays an important role in the inhibition of host innate immune response. Degrades host elastin, fibrogen, fibronectin and kininogen. Blocks phagocytosis of opsonised S. aureus by neutrophils and monocytes by inducing their death in a proteolytic activity-dependent manner. Decreases surface expression of the 'don't eat me' signal CD31 on neutrophils. Cleaves host galectin-3/LGALS3, thereby inhibiting the neutrophil-activating ability of the lectin.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase C47 family
  • 数据库链接：

    KEGG: sao:SAOUHSC_00987

    STRING: 93061.SAOUHSC_00987