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  4. Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

  • 货号：
    CSB-YP323941SHD
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP323941SHD-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP323941SHD
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP323941SHD
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    vpx
  • Uniprot No.：
  • 别名：
    vpxProtein Vpx; Viral protein X; X ORF protein
  • 种属：
    Simian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-112
  • 氨基酸序列
    MSDPRERIPP GNSGEETIGE AFDWLDRTVE EINRAAVNHL PRELIFQVWR RSWEYWHDEM GMSVSYTKYR YLCLIQKAMF MHCKKGCRCL GGEHGAGGWR PGPPPPPPPG LA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have purchased your vpx recombinant protein a long time ago (CSB-EP323941SHD; Lot#: E051453) and I would like to see the COA for the batch we received. Can you please provide this COA. Also, was the protein sent to us liquid or lyophilized? Please provide the formulation of the buffer that was used as well as Endotoxin level (if tested).

A:
Very nice to receive your inquiry. I have checked your order record and the details are as below.
Name: Recombinant Simian immunodeficiency virus Protein Vpx (vpx)
CUSABIO Code: CSB-EP323941SHD
Order Time: September 5th, 2014
Size: 5mg
Seq Pos: 1-112
Sequence:

MSDPRERIPPGNSGEETIGEAFDWLDRTVEEINRAAVNHLPRELIFQVWRRSWEYWHDEMGMSVSYTKYR YLCLIQKAMFMHCKKGCRCLGGEHGAGGWRPGPPPPPPPGLA


Tag Information: His tagged
Unit Size: 5 mg (E Coli Derived)
This protein was delivered in year 2014. We didn't provide COA at that time and could only provide datasheet (attached).
The formulation of the buffer is 20mM Tris-HCl, 0.5M NaCl, PH 8.0,50% glycerol.
You didn't require endotoxin removal when placing the order, so didn't test the endotoxin level.
Q:

We recently received your protein CSB-EP323941SHD, lot 03767, which was concerned because this lot was more concentrated than the previous lots they received. We want to verify the concentration, and were wondering if you had a recommended commercial assay that we would be able to purchase to confirm the concentration found by your team. Would you be able to advise on the Bradford kit you guys used to determine the concentration of this lot?

A:
Thanks for your inquiry. As shown in our Datasheet&COA, the method we used to determine the protein concentration is Bradford Method, and the standard we used is BSA.
We determined this group of solutions by formulating a set of bovine serum albumin (BSA) solutions at concentrations of 0.10 mg/ml, 0.08 mg/ml, 0.06 mg/ml, 0.04 mg/ml, 0.02 mg/ml, and 0 mg/ml, respectively.
The absorbance gives a standard curve of protein concentration versus absorbance. The absorbance of the sample of unknown protein concentration was determined, and the concentration of the protein was
obtained according to a standard curve. At the same time, we also combined SDS-PAGE observations to determine the target protein concentration.
For your problem "Lot03767 was more concentrated than the previous lots we received". Would you please confirm which lots you had received before?
Q:

Lot# 03767 concentration question. Hello, My Certificate of analysis for this lot says 0.3 mg/ml. This is my third lot that I have received in the past year, and this lot seems to be more potent than the other two lots when run in the same assay. I see that you test concentration by using the Bradford Method. I have two topics that I would like to know more about: 1) Do you have more specific data for this lot--was it closer to 0.34 mg/mL--or does this assay only give results to one significant figure? 2) Do you use a commercial test kit to measure the concentration? I see that Thermal Fisher Life Technologies has several types of test kits made by Pierce available. Do you know if their cat #23236 Coomassie Plus (Bradford) Assay Kit will give similar concentration results as what you perform? Do you have a suggestion of a commercial test kit that I could use to check the protein concentration myself? Thank you for your help with this inquiry.
Current lot→0.3mg/ml
Lot# 03614→0.8mg/ml
Lot# 03433→0.4mg/ml

A:
Thanks for your quick response.
1. Yes. The result gives an approximation that is not a very certain value.
So whether it is close to 0.34 mg/ml, there is such a possibility, but we can't confirm it yet, and there may be some deviations in different concentration detection methods.
2. We did not use a commercial test kit to measure the concentration.
If you want to verify, you can buy a commercial test kit to detect relevant protein concentration, and we will be grateful if you finally share the test results with us.

靶点详情

  • 功能：
    Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription.
  • 亚细胞定位：
    Virion. Host nucleus.
  • 蛋白家族：
    Lentivirus VPX protein family