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  4. Recombinant Schizosaccharomyces pombe WD repeat-containing protein wat1 (wat1)

Recombinant Schizosaccharomyces pombe WD repeat-containing protein wat1 (wat1)

  • 货号：
    CSB-YP529159SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP529159SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP529159SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP529159SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP529159SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    pop3; wat1; SPBC21B10.05c; Target of rapamycin complex subunit wat1; TORC subunit wat1; WD repeat-containing protein pop3
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-314
  • 氨基酸序列
    MSVQYPPQHS VLLVSSGYDH TIRFWEALSG ICSRTIQHAD SQVNRLCISP DKKFLAAAGN PHVRLYDINT SSQMPLMTFE GHTNNVTAIA FHCDGKWLAT SSEDGTVKVW DMRAPSVQRN YDHKSPVNDL LIHPNQGELL SCDQSGRVRA WDLGENSCTH ELIPEEDVPM SSITVGSDGS MLIAGNNKGN CYVWRMLNHQ GASLLQPVVK FQAHQRYITR CVLSPDVKHL ATCSADATVN IWSTEDMSFM LERRLQGHQR WVWDCAFSAD STYLVTASSD HVARLWELSS GETIRQYSGH HKAAVCVALN DYQI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    component of both TORC1 and TORC2, which regulate multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls cell growth and ribosome biogenesis by regulating ribosomal protein gene expression. TORC1 negatively controls G1 cell-cycle arrest, sexual development and amino acid uptake. Represses mating, meiosis and sporulation efficiency by interfering with the functions of the transcription factor ste11 and the meiosis-promoting RNA-binding protein mei2. TORC2 is required for cell survival under various stress conditions. TORC2 positively controls G1 cell-cycle arrest, sexual development and amino acid uptake. Positively regulates amino acid uptake through the control of expression of amino acid permeases. May play a role in mRNA maturation as a coupling protein between splicing and synthesis and/or stabilization.
  • 基因功能参考文献：
    1. In response to osmotic stress Wat1 undergoes hyper-phosphorylation at S116 position. Wat1 interacts with Tor1 and Gad8. PMID: 29699848
    2. In the absence of wat1, the cells exhibited a high level of nuclear fragmentation. wat1 is required for the regulation of the oxidative stress response. chk1- and wat1-null mutants exhibit conditional synthetic lethality. PMID: 27683273
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    WD repeat LST8 family
  • 数据库链接：

    KEGG: spo:SPBC21B10.05c

    STRING: 4896.SPBC21B10.05c.1