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  4. Recombinant Schizosaccharomyces pombe Switch-activating protein 1 (sap1)

Recombinant Schizosaccharomyces pombe Switch-activating protein 1 (sap1)

  • 中文名称：
    庞贝裂殖酵母sap1重组蛋白
  • 货号：
    CSB-YP331232SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母sap1重组蛋白
  • 货号：
    CSB-EP331232SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母sap1重组蛋白
  • 货号：
    CSB-EP331232SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母sap1重组蛋白
  • 货号：
    CSB-BP331232SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母sap1重组蛋白
  • 货号：
    CSB-MP331232SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sap1
  • Uniprot No.：
  • 别名：
    sap1; SPCC1672.02c; Switch-activating protein 1
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-254
  • 氨基酸序列
    MEAPKMELKS YKRKNASLSP SSSPAKAQRT HLSLEEKIKL MRLVVRHKHE LVDRKTSEFY AKIARIGYED EGLAIHTESA CRNQIISIMR VYEQRLAHRQ PGMKTTPEED ELDQLCDEWK ARLSELQQYR EKFLVGKRKC DCNDEINERL KKLTEEQQNV DMLVAKVNFL SKHLHDNEEK LMQVNAKMDE VLAENKRLQQ LLDHNDLLSK LEPPSAYAPH GVNMGTNMGA NMGANMNAIR GGLHSSISPN LGDH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to sequences required for mating-type switching. Makes a simultaneous contact with both the alpha and beta domains of the switch-activating site SAS1. Also binds to replication fork barrier 1 (RFB1) located within a 78 base pair sequence near the 3' end of the rRNA coding region. This leads to replication fork blockage. It binds the consensus sequence 5'-TA[AG]GCAGNTN[CT]AACG[AC]G-3'.; Has a role in chromosome organization and integrity where it is involved in chromosome segregation. Has a role in sister chromatid cohesion and condensation.
  • 基因功能参考文献：
    1. Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA-replication origins in fission yeast are defined by possessing two essential elements with one bound by ORC and the other by Sap1. PMID: 28223353
    2. This study shows that insertion of the fission yeast LTR retrotransposon Tf1 is guided by the DNA binding protein Sap1 and that the efficiency and location of the targeting depend on the activity of Sap1 as a replication fork barrier. PMID: 26404838
    3. Sap1p binds the rDNA fork barrier Ter1 to cause polar replication fork arrest at this site but not at SAS1. PMID: 16195226
    4. Results reveal that Sap1-DNA affinity alone is insufficient to account for fork arrest and suggest that Sap1 binding-induced structural changes may result in formation of a competent fork-blocking complex. PMID: 16940176
    5. Sap1 is required to recruit the replication fork protection complex to chromatin, thereby contributing to the activation of the replication checkpoint and the stabilization of replication forks. PMID: 17151242
    6. Sap1 is required for replication fork arrest at the rRNA genes of fission yeast PMID: 16166653

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: spo:SPCC1672.02c

    STRING: 4896.SPCC1672.02c.1