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  4. Recombinant Schizosaccharomyces pombe Cofilin (cof1)

Recombinant Schizosaccharomyces pombe Cofilin (cof1)

  • 货号：
    CSB-YP304429SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP304429SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP304429SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP304429SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP304429SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cof1
  • Uniprot No.：
  • 别名：
    cof1; adf1; SPAC20G4.06cCofilin; Actin-depolymerizing factor 1
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-137
  • 氨基酸序列
    MSFSGVKVSP ECLEAFQELK LGKSLRYVVF KMNDTKTEIV VEKKSTDKDF DTFLGDLPEK DCRYAIYDFE FNLGEGVRNK IIFISWSPDV APIKSKMVYS SSKDTLRRAF TGIGTDIQAT DFSEVAYETV LEKVTRK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. Binding to F-actin is regulated by tropomyosin. It is the major component of intranuclear and cytoplasmic actin rods. Required for accumulation of actin at the cell division site via depolymerizing actin at the cell ends. In association with myosin II has a role in the assembly of the contractile ring via severing actin filaments. Involved in the maintenance of the contractile ring once formed. In association with profilin and capping protein, has a role in the mitotic reorganization of the actin cytoskeleton.
  • 基因功能参考文献：
    1. Cofilin rapidly dissociates Arp2/3 complex and branches by direct competition for binding sites on the actin filament and by propagation of structural changes in the actin filament that reduce affinity for Arp2/3 complex. PMID: 19362000
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Note=Septum. Throughout the cytoplasm (but not on the cytoplasmic cables) and major component of the cortical actin cytoskeleton.
  • 蛋白家族：
    Actin-binding proteins ADF family
  • 数据库链接：

    KEGG: spo:SPAC20G4.06c

    STRING: 4896.SPAC20G4.06c.1