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  4. Recombinant Schizosaccharomyces pombe Chromatin-associated protein swi6 (swi6)

Recombinant Schizosaccharomyces pombe Chromatin-associated protein swi6 (swi6)

  • 中文名称：
    庞贝裂殖酵母swi6重组蛋白
  • 货号：
    CSB-YP340241SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母swi6重组蛋白
  • 货号：
    CSB-EP340241SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母swi6重组蛋白
  • 货号：
    CSB-EP340241SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母swi6重组蛋白
  • 货号：
    CSB-BP340241SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母swi6重组蛋白
  • 货号：
    CSB-MP340241SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    swi6
  • Uniprot No.：
  • 别名：
    swi6; SPAC664.01c; SPAC824.10c; Chromatin-associated protein swi6
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-328
  • 氨基酸序列
    MKKGGVRSYR RSSTSKRSVI DDDSEPELPS MTKEAIASHK ADSGSSDNEV ESDHESKSSS KKLKENAKEE EGGEEEEEDE YVVEKVLKHR MARKGGGYEY LLKWEGYDDP SDNTWSSEAD CSGCKQLIEA YWNEHGGRPE PSKRKRTARP KKPEAKEPSP KSRKTDEDKH DKDSNEKIED VNEKTIKFAD KSQEEFNENG PPSGQPNGHI ESDNESKSPS QKESNESEDI QIAETPSNVT PKKKPSPEVP KLPDNRELTV KQVENYDSWE DLVSSIDTIE RKDDGTLEIY LTWKNGAISH HPSTITNKKC PQKMLQFYES HLTFRENE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Involved in the repression of the silent mating-type loci MAT2 and MAT3. May compact MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes.
  • 基因功能参考文献：
    1. rather than promoting maintenance and spreading of heterochromatin, Swi6 appears to limit these processes and appropriately confine heterochromatin. PMID: 26438724
    2. binding of HP1 protein, Swi6, to methylated nucleosomes drives a switch from an auto-inhibited state to a spreading-competent state, where H3K9-methylated heterochromatin spreads to adjacent regions PMID: 23485968
    3. HP1(Swi6) mediates the recognition and destruction of heterochromatic RNA transcripts PMID: 22683269
    4. Mutations in Swi6 that disrupt cdc6 protein interaction disrupt silencing and delay replication in the centromere. PMID: 21239883
    5. Our analyses suggest that Swi6/HP1 recruits opposing chromatin-modifying activities, the balancing of which is crucial for heterochromatin maintenance. PMID: 16762840
    6. Binding of Swi6 within approx 50kb of the telomere occurs less in partial aneuploid strains compared to euploid strains of S. pombe. PMID: 18285637
    7. These results provide insights into the regulation of siRNA action and reveal roles for cis-dsRNA synthesis and HP1 in siRNA-mediated heterochromatin assembly. PMID: 18657501
    8. casein kinase II (CK2) phosphorylated Swi6 PMID: 19136623
    9. Msc1, is a critical factor required for proper cell fate determination in S. pombe. PMID: 19164572
    10. Swi6, an S. pombe counterpart of heterochromatin protein 1 (HP1), is required for early replication of the pericentromeric region and the mat locus. PMID: 19182789
    11. These analyses suggest that among other functions, HP1 proteins associate with chromatin-modifying factors that in turn cooperate to assemble repressive chromatin; thus, precluding accessibility of underlying DNA sequences to transcriptional machinery. PMID: 19443688

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: spo:SPAC664.01c

    STRING: 4896.SPAC664.01c.1