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  4. Recombinant Salmonella typhimurium RNA polymerase sigma-E factor (rpoE)

Recombinant Salmonella typhimurium RNA polymerase sigma-E factor (rpoE)

  • 中文名称：
    鼠伤寒沙门菌rpoE重组蛋白
  • 货号：
    CSB-YP358217SXB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌rpoE重组蛋白
  • 货号：
    CSB-EP358217SXB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌rpoE重组蛋白
  • 货号：
    CSB-EP358217SXB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    鼠伤寒沙门菌rpoE重组蛋白
  • 货号：
    CSB-BP358217SXB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌rpoE重组蛋白
  • 货号：
    CSB-MP358217SXB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpoE
  • Uniprot No.：
  • 别名：
    rpoE; sigE; STM2640; ECF RNA polymerase sigma-E factor; RNA polymerase sigma-E factor; Sigma-24
  • 种属：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-191
  • 氨基酸序列
    MSEQLTDQVL VERVQKGDQK AFNLLVVRYQ HKVASLVSRY VPSGDVPDVV QESFIKAYRA LDSFRGDSAF YTWLYRIAVN TAKNYLVAQG RRPPSSDVDA IEAENFESGG ALKEISNPEN LMLSEELRQI VFRTIESLPE DLRMAITLRE LDGLSYEEIA AIMDCPVGTV RSRIFRAREA IDNKVQPLIR R
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as acid stress, heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment.
  • 基因功能参考文献：
    1. this is the first report demonstrating that RpoE acts as a regulator in the expression of Agf in Salmonella. PMID: 23159630
    2. the extracytoplasmic sigma factor sigma(E) (RpoE) is required for Salmonella resistance to killing by the bactericidal/permeability-increasing protein (BPI)-derived peptide P2 and the murine alpha-defensin cryptdin-4 PMID: 15819632
    3. sigma(E) augments sigma(S) levels during stationary phase via enhanced expression of sigma(H) and the RNA-binding protein Hfq, leading to increased expression of sigma(S)-dependent genes and enhanced resistance to oxidative stress. PMID: 15819634
    4. Nucleotides critical for the activity of the rpoEp3 promoter are identified. PMID: 16842358
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sigma-70 factor family, ECF subfamily
  • 数据库链接：

    KEGG: stm:STM2640

    STRING: 99287.STM2640