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  4. Recombinant Saccharomyces cerevisiae Vacuolar protein-sorting-associated protein 24 (VPS24)

Recombinant Saccharomyces cerevisiae Vacuolar protein-sorting-associated protein 24 (VPS24)

  • 中文名称：
    酿酒酵母VPS24重组蛋白
  • 货号：
    CSB-YP334077SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母VPS24重组蛋白
  • 货号：
    CSB-EP334077SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母VPS24重组蛋白
  • 货号：
    CSB-EP334077SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母VPS24重组蛋白
  • 货号：
    CSB-BP334077SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母VPS24重组蛋白
  • 货号：
    CSB-MP334077SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VPS24
  • Uniprot No.：
  • 别名：
    VPS24; DID3; YKL041W; YKL254; Vacuolar protein-sorting-associated protein 24; DOA4-independent degradation protein 3; ESCRT-III complex subunit VPS24
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-224
  • 氨基酸序列
    MDYIKKAIWG PDPKEQQRRI RSVLRKNGRN IEKSLRELTV LQNKTQQLIK KSAKKNDVRT VRLYAKELYQ INKQYDRMYT SRAQLDSVRM KIDEAIRMNT LSNQMADSAG LMREVNSLVR LPQLRNTMIE LEKELMKSGI ISEMVDDTME SVGDVGEEMD EAVDEEVNKI VEQYTNEKFK NVDQVPTVEL AANEEEQEIP DEKVDEEADR MVNEMRERLR ALQN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment oflate-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. The DID4/VPS2-VPS24 subcomplex is required for the VPS4-dependent dissociation of ESCRT-III.
  • 基因功能参考文献：
    1. Impact of Vta1 and ESCRT-III family members on Vps4 ATPase activity. PMID: 18194652
    2. Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24. PMID: 18786397
  • 亚细胞定位：
    Endosome membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Note=Endosomal and other punctate structures.
  • 蛋白家族：
    SNF7 family
  • 数据库链接：

    KEGG: sce:YKL041W

    STRING: 4932.YKL041W