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  4. Recombinant Saccharomyces cerevisiae Securin (PDS1)

Recombinant Saccharomyces cerevisiae Securin (PDS1)

  • 货号：
    CSB-YP019074SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019074SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019074SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019074SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019074SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PDS1
  • Uniprot No.：
  • 别名：
    PDS1; YDR113C; YD9727.08C; Securin
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-373
  • 氨基酸序列
    MMPANEDKEN NIVYTGNESS GINFPQTPAH LLKRSHSNIL KPPVRLDQLK RDANSNNGNT LKYIQGGKEV SPTKRLHTHA QQQGRLPLAA KDNNRSKSFI FPETSNQSKD ADLPQLQNTL SIRKNDQLRK LSQISRSRSR ANHNDLLSNS RKLQKYGSVL GYNALPKMKS LVLKDLADSG KNEESSDDDE GNEDSESKLG KKLQSALLKQ DSSDGENELN GGLGLFNEQG GLQQLIKNST KNEQKTKNDK SDKTDDYDIE IAPQRQEPLP YVPEGYSPFQ QDDIEKLKTF NSPYKLDLED EDDTPDKVDL LPLEQIDEEG EKDETECITR NQEEGAALPL LSKNFKEVAA VPTMELVYSE EGLDPEELED LVT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1.
  • 基因功能参考文献：
    1. crystal structures at up to 2.6 A resolution of the yeast Saccharomyces cerevisiae separase-securin complex PMID: 28146474
    2. These data indicate that Clb5 and Clb6 promote cohesion which is then protected by Pds1 and that both mechanisms are required during replication stress. PMID: 21518961
    3. Chk1-Pds1 checkpoint has roles in preventing tolerance of persistent single-strand breaks in Saccharomyces cerevisiae PMID: 15687272
    4. protein phosphatase 2A regulates sister chromatid cohesion in Pds1-dependent and -independent manners PMID: 17050679
    5. phosphorylation of Pds1 but not Rad53 correlates with G2 arrest in response to double-strand breaks in the mec1 hypomorphic mutant PMID: 17671432
    6. RAD9, CHK1 and PDS1 are required for elevated homologous recombination in a mec1 (ATR) hypomorphic mutant. PMID: 18677117
    7. These results define a previously undescribed role for Pds1p in cohesin maintenance, recombination, and meiotic progression. PMID: 19001291

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Securin family
  • 数据库链接：

    KEGG: sce:YDR113C

    STRING: 4932.YDR113C