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  4. Recombinant Regulatory protein E2 (E2)

Recombinant Regulatory protein E2 (E2)

  • 货号：
    CSB-YP355956BKJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP355956BKJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP355956BKJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP355956BKJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP355956BKJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    E2
  • Uniprot No.：
  • 别名：
    E2; Regulatory protein E2
  • 种属：
    Bovine papillomavirus type 1
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-410
  • 氨基酸序列
    METACERLHV AQETQMQLIE KSSDKLQDHI LYWTAVRTEN TLLYAARKKG VTVLGHCRVP HSVVCQERAK QAIEMQLSLQ ELSKTEFGDE PWSLLDTSWD RYMSEPKRCF KKGARVVEVE FDGNASNTNW YTVYSNLYMR TEDGWQLAKA GADGTGLYYC TMAGAGRIYY SRFGDEAARF STTGHYSVRD QDRVYAGVSS TSSDFRDRPD GVWVASEGPE GDPAGKEAEP AQPVSSLLGS PACGPIRAGL GWVRDGPRSH PYNFPAGSGG SILRSSSTPV QGTVPVDLAS RQEEEEQSPD STEEEPVTLP RRTTNDGFHL LKAGGSCFAL ISGTANQVKC YRFRVKKNHR HRYENCTTTW FTVADNGAER QGQAQILITF GSPSQRQDFL KHVPLPPGMN ISGFTASLDF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.
  • 基因功能参考文献：
    1. Results for this mutational analysis suggest that the transactivation domain dimerization interface may contribute to the DNA replication activity of bovine papillomavirus type 1 E2 protein PMID: 23490049
    2. s propose a model by which bovine papillomavirus E2 transcriptional activity is controlled by bovine p300-mediated acetylation of lysine 111. PMID: 23152516
    3. Thus, the bovine papillomavirus-1 E2-TR modulates viral gene expression in a manner distinct from other E2 proteins. PMID: 22551766
    4. These data provide evidence that the association of bovine papillomavirus E2 with human ChlR1 contributes to a loading mechanism during DNA replication rather than direct tethering during mitotic division. PMID: 21489590
    5. results indicate that precise folding of the E2 transactivation domain is crucial for its interaction with mitotic chromosomes and that this association can be modulated PMID: 15650176
    6. Brd4 has recently been proposed to mediate the association between bovine papilomavirus1 E2 and mitotic chromosomes. PMID: 15795266
    7. E2 proteins that could dimerize were able to bind to mitotic chromosomes much more efficiently than monomeric E2 polypeptides. PMID: 18495759
    8. E2 heterodimers with single activation domain are functional in initiation of papillomavirus replication in vivo. PMID: 19232665
    9. BPV E2 remaines attached to definable segments of the viral genome at specific stages of the cell cycle. PMID: 19716579

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  • 亚细胞定位：
    Host nucleus. Note=Colocalizes with DDX11 at early stages of mitosis.
  • 蛋白家族：
    Papillomaviridae E2 protein family
  • 数据库链接：

    KEGG: vg:1489020