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  4. Recombinant Rat X-box-binding protein 1 (Xbp1)

Recombinant Rat X-box-binding protein 1 (Xbp1)

  • 货号：
    CSB-YP865646RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP865646RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP865646RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP865646RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP865646RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Xbp1; Htf; X-box-binding protein 1; Hepatocarcinogenesis-related transcription factor; HTF) [Cleaved into: X-box-binding protein 1; cytoplasmic form; X-box-binding protein 1; luminal form]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-267
  • 氨基酸序列
    MVVVAAAPSA ASAAPKVLLL SGQPASGGRA LPLMVPGPRA AGSEASGTPQ ARKRQRLTHL SPEEKALRRK LKNRVAAQTA RDRKKARMSE LEQQVVDLEE ENQKLQLENQ LLREKTHGLV IENQELRTRL GMNALVTEEV SEAESKGNGV RLVAGSAESA ALRLRAPLQQ VQAQLSPPQN IFPWILTLLP LQILSLISFW AFWTSWTLSC FSNVLPQSLL IWRNSQRSTQ KDLVPYQPPF LCQWGPHQPS WKPLMNSFVL TMYTPSL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as a transcription factor during endoplasmic reticulum (ER) stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development, and the development of secretory tissues such as exocrine pancreas and salivary gland. Involved in terminal differentiation of B lymphocytes to plasma cells and production of immunoglobulins. Modulates the cellular response to ER stress in a PIK3R-dependent manner. Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes. Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions. Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention.; Acts as a weak transcriptional factor. Together with HDAC3, contributes to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to EC survival under disturbed flow/oxidative stress. Binds to the ER stress response element (ERSE) upon ER stress. Binds to the consensus 5'-GATGACGTG[TG]N(3)[AT]T-3' sequence related to cAMP responsive element (CRE)-like sequences. Associates preferentially to the HDAC3 gene promoter region in a static flow-dependent manner. Binds to the CDH5/VE-cadherin gene promoter region.
  • 基因功能参考文献：
    1. These results suggest that overexpression of activated XBP1s protects against neuronal cell death following O3 exposure and that activation of the XBP1s pathway may offer a preventative way for prophylactic treatment of spinal cord neurons exposed to O3. PMID: 29956773
    2. XBP-1 and PARP protein are sensitive to variations in fluorine concentration, which indicates that oxidative stress from fluorine exposure can lead to apoptosis. XBP-1 and PARP may be the key proteins during the entire process. PMID: 26883836
    3. Enhanced splicing of XBP-1 was observed in BCL-2 overexpressing cells implicating BCL-2 in the complex regulation of IRE1alpha activity. PMID: 26319553
    4. that XBP1 is an important gene involved in regulation of the anti-oxidant defense in the RPE, and that impaired activation of XBP1 may contribute to RPE dysfunction and cell death during retinal degeneration and AMD. PMID: 22715395
    5. XBP1 lymphocyte expression is down-regulated under conditions of psychological stress in rats. PMID: 25007519
    6. XBP1s is linked to suppressed gluconeogenesis in the Ebb phase of burn injury. PMID: 23508570
    7. The study provides evidence that spliced XBP1 (XBP1S) pathway of endoplasmic reticulum stress was suppressed in high glucose-treated renal mesangial cells and renal cortex of diabetic rats. PMID: 23457509
    8. Failure of the transgenic unfolded protein response and XBP1 are implicated in DNA binding protein TAR(TDP)-43 pathogenesis. PMID: 22970712
    9. Found that suppression of XBP1 activation accelerates neuronal cell death after ischemia/reperfusion. PMID: 22580202
    10. Mild endoplasmic reticulum stress augments the proinflammatory effect of IL-1beta in pancreatic rat beta-cells via the IRE1alpha/XBP1s pathway PMID: 22529213
    11. Partial knockdown of XBP1 is not sufficient to protect precursor oligodendrocytes from ischemic damage. PMID: 21337367
    12. Hepatic nuclear factor 1alpha (HNF1alpha) dysfunction down-regulates X-box-binding protein 1 (XBP1) and sensitizes beta-cells to endoplasmic reticulum stress. PMID: 21784843
    13. X-box binding protein 1 regulates brain natriuretic peptide through a novel AP1/CRE-like element in cardiomyocytes PMID: 20170659
    14. Binding of Hsp72 to IRE1alpha enhances IRE1alpha/XBP1 signaling at the endoplasmic reticulum (ER) and inhibits ER stress-induced apoptosis. PMID: 20625543
    15. XBP1 has a distinct inhibitory role in beta cell as compared with other cell types. Prolonged XBP1s production hampers beta cell function via inhibition of insulin, Pdx1 and Mafa expression, eventually leading to beta cell apoptosis. PMID: 20349222
    16. In hippocampal neurons after brain ischemia and reperfusion injury, only Xbp1 and chop showed increases in mRNA. PMID: 17715997
    17. expression correlates positively with percent volume of ameloblast endoplasmic reticulum PMID: 18946015

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  • 亚细胞定位：
    Nucleus. Endoplasmic reticulum.; [Isoform 1]: Nucleus. Cytoplasm. Endoplasmic reticulum membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    BZIP family
  • 数据库链接：

    KEGG: rno:289754

    STRING: 10116.ENSRNOP00000014044

    UniGene: Rn.101044