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  4. Recombinant Rat Von Hippel-Lindau disease tumor suppressor (Vhl)

Recombinant Rat Von Hippel-Lindau disease tumor suppressor (Vhl)

  • 货号：
    CSB-YP727052RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP727052RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP727052RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP727052RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP727052RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Vhl; von Hippel-Lindau disease tumor suppressor; pVHL
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-185
  • 氨基酸序列
    MPRKAASPEE AERMPGSEEI EAGRPRPVLR SVNSREPSQV IFCNRSPRVV LPLWLNFDGE PQPYPTLPPG TGRRIHSYRG HLWLFRDAGT HDGLLVNQTE LFVPSLNVDG QPIFANITLP VYTLKERCLQ VVRSLVKPEN YRRLDIVRSL YEDLEDHPNV RKDIQRLTQE HLENQALGEE PEGVH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.
  • 基因功能参考文献：
    1. VHL functions to induce neuronal differentiation of neural stem/progenitor cells and skin-derived precursors. PMID: 29401731
    2. that the change of VHL protein expression was related to neuronal apoptosis after spinal cord injury PMID: 27324785
    3. we observed that microtubule network stabilization downregulated Ran-mediated pVHL nuclear accumulation, which in turn led to the upregulation of HIF-1alpha protein PMID: 25779090
    4. The protein levels of HIF-1alpha were down-regulated through overexpression of pVHL and were up-regulated through knockdown of pVHL in hypoxic cardiomyocytes. PMID: 22506063
    5. VHL peptide-delivered MSCs differentiated into neuron-like cells, and that engrafted VHL peptide-delivered marrow stromal cells (MSCs) more recovered the behaviors of the rats than that of nondelivered MSCs. PMID: 20125055
    6. Endogenous von Hippel-Lindau tumor suppressor protein regulates catecholaminergic phenotype in PC12 cells. PMID: 11912140
    7. pVHL specifically binds the hyperphosphorylated Rpb1 in a proline-hydroxylation-dependent manner, targeting it for ubiquitination PMID: 12604794
    8. Tyrosine hydroxylase promoter activity is regulated by the von Hippel-Lindau tumor suppressor protein and HIF1alpha and HIF2alpha in PC12 cells PMID: 12675925
    9. Significantly greater axonal outgrowth was observed for the Von Hippel-Lindau protein-transfected cells treated with the inhibitor Lavendustin A than for those not so treated. PMID: 15094462
    10. Hypoxia induction or normoxic acidosis can neutralize the function of VHL by triggering its nucleolar sequestration, a regulatory mechanism of protein function that is observed rarely. PMID: 15181450
    11. Von Hippel-Lindau protein (VHL) pre-induction by thrombin aggravates glomerulonephritis (GN), and the increase in VHL expression due to Habu snake venom might be involved in accelerating onset of GN. PMID: 17519558

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  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein. Nucleus.
  • 蛋白家族：
    VHL family
  • 数据库链接：

    KEGG: rno:24874

    STRING: 10116.ENSRNOP00000013727

    UniGene: Rn.11059