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  4. Recombinant Rat Vitamin D3 receptor (Vdr)

Recombinant Rat Vitamin D3 receptor (Vdr)

  • 货号：
    CSB-YP025832RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025832RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025832RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025832RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025832RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Vdr
  • Uniprot No.：
  • 别名：
    Vdr; Nr1i1Vitamin D3 receptor; VDR; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-423
  • 氨基酸序列
    MEATAASTSL PDPGDFDRNV PRICGVCGDR ATGFHFNAMT CEGCKGFFRR SMKRKALFTC PFNGDCRITK DNRRHCQACR LKRCVDIGMM KEFILTDEEV QRKREMIMKR KEEEALKDSL RPKLSEEQQH IIAILLDAHH KTYDPTYADF RDFRPPVRMD GSTGSYSPRP TLSFSGNSSS SSSDLYTTSL DMMEPSGFSN LDLNGEDSDD PSVTLDLSPL SMLPHLADLV SYSIQKVIGF AKMIPGFRDL TSDDQIVLLK SSAIEVIMLR SNQSFTMDDM SWDCGSQDYK YDVTDVSKAG HTLELIEPLI KFQVGLKKLN LHEEEHVLLM AICIVSPDRP GVQDAKLVEA IQDRLSNTLQ TYIRCRHPPP GSHQLYAKMI QKLADLRSLN EEHSKQYRSL SFQPENSMKL TPLVLEVFGN EIS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis.
  • 基因功能参考文献：
    1. Vitamin D inhibits lymphangiogenesis through VDR-dependent mechanisms. PMID: 28303937
    2. VDR and PDIA3/1,25MARRS genes were silenced separately or simultaneously in E16 primary rat cortical neurons PMID: 28707894
    3. it is conceivable that the activation of the ERK1/2 pathway induced by VDR activation may have an essential role in the evasion of apoptosis after global cerebral ischemia . PMID: 29138801
    4. These results demonstrated the localization of VDR on the neuronal plasma membrane and the co-localization of VDR and APP or ADAM10 or Nicastrin and limited co-localization of VDR and PS1. PMID: 29176823
    5. vitamin D receptor analog VS-105 and paricalcitol have an effect on chronic kidney disease-mineral bone disorder in an experimental model of chronic kidney disease PMID: 27818277
    6. Liganded vitamin D receptor through its interacting repressor inhibits the expression of Col1a1. PMID: 27351590
    7. Renal VDR expression is decreased in spontaneously hypertensive rats before the development of hypertension. PMID: 27009470
    8. Acute bout of resistance exercise increases vitamin D receptor protein expression in rat skeletal muscle. PMID: 26347486
    9. VDR may mediate the increased expression levels of BMP2, Runx2 and Osterix by positively regulating calcium levels in primary primary renal tubular epithelial cells. PMID: 25823394
    10. Antenatal endotoxin disrupts lung vitamin D receptor and 25-hydroxyvitamin D 1alpha-hydroxylase expression in the developing rat. PMID: 26342089
    11. Thus, VDR in the apical brush border of the proximal convoluted tubule cells serves to "sense" the level of circulating 1,25(OH)2D3 and modulates the activity of the 1alpha-hydroxylase and the 24-hydroxylase accordingly. PMID: 25425001
    12. VS-105 may provide cardiovascular benefits in 5/6 nephrectomized rats via VDR activation. PMID: 25503724
    13. Show VDR in the adult rodent brain using proteomic techniques; in the embryo VDR distribution is most prominent in the nucleus; by adulthood that this has reversed with a prominent membrane component for VDR in the gut and kidney but not the brain PMID: 24607320
    14. these findings suggest that functional cooperation between Vdr and Runx2 is necessary for vascular calcification in response to vitamin D3. PMID: 24349534
    15. A significant increase in VDR expression was observed in DRG neurons of diabetic rats. PMID: 23684983
    16. crystal structures of the ligand-binding domain of rat VDR (VDR-LBD) in ternary complexes with a synthetic partial peptide of the coactivator MED1 (mediator of RNA polymerase II transcription subunit 1) and four ligands PMID: 23723390
    17. Calcitriol promotes vascular calcification through systemic vitamin D receptors rather than vascular receptors. PMID: 24202304
    18. we confirm that the VDR is present in the nucleus of tyrosine hydroxylase (TH)-positive neurons in both the human and rat substantia nigra PMID: 23352937
    19. Data indicate that ligands changed the conformation of the vitamin D receptor (VDR), resulting in different hydrogen-bond networks depending on the potency of the ligand. PMID: 23462137
    20. There is a role for VDR in increasing the brain clearance of P-gp substrates, including human amyloid Abeta42 protein, a plaque-forming precursor in Alzheimer's disease. PMID: 23035695
    21. In studies of conformational changes in ligand-binding domain of VDR, NMR data suggest that ligand-specific chemical shifts map not only to residues at/near binding pocket but also to residues remote from ligand-binding site. PMID: 22112050
    22. Over-expression of VDR in cellular plasma and nuclear membranes of osteoblasts was found in bone of immature rats with disuse osteoporosis. PMID: 20369481
    23. Nuclear vitamin D receptor immunoreactivity was present within nearly all neurons, while cytoplasmic VDR was found preferentially in unmyelinated calcitonin gene-related peptide (CGRP)-positive neurons, colocalizing with CYP27B1 and CYP24. PMID: 20969950
    24. analysis of the effect of the vitamin D analog, paricalcitol, a VDR activator (VDRA), on the progression of cardiomyopathy in the 5/6 nephrectomized uremic model PMID: 20236614
    25. association between VDR and caveolin-3 and the regulation of this interaction by 1,25(OH)2D3 are fundamentally important in understanding 1,25(OH)2D3 signal transduction in heart cells PMID: 20304057
    26. Hr and VDR interact via multiple protein-protein interfaces, catalyzing histone demethylation to effect chromatin remodeling and repress the transcription of VDR target genes that control the hair cycle. PMID: 20512927
    27. Enhancement of VDR-mediated transcription by phosphorylation: correlation with increased interaction between the VDR and DRIP205, a subunit of the VDR-interacting protein coactivator complex PMID: 11818502
    28. Tissue-specific down-regulation of VDR by hypocalcemia blocks the 1,25-(OH)(2)D(3) suppression of the 1alpha-OHase and upregulation of the 24-OHase in the kidney, causing a marked accumulation of 1,25-(OH)(2)D(3) in the plasma. PMID: 12054486
    29. VDR protein localizes in the nuclei of cardiac muscle fibres; VDR mRNA expression changes over different periods of development from embryo to adult PMID: 12107506
    30. ICER has a key regulatory role in the PKA enhancement of VDR transcription and therefore in the cross-talk between the PKA signaling pathway and the vitamin D endocrine system PMID: 12198242
    31. the effect of estrogen and progesterone on the expression of dihydroxyvitamin D3 receptor (VDR) mRNA in the liver of ovariectomized rats PMID: 12297474
    32. Extracellular Ca regulates vitamin D receptor expression by parathyroid cells independently of calcitriol and by this mechanism hypocalcemia may prevent the feedback of calcitriol on the parathyroids. PMID: 12444213
    33. rat hepatocytes express very low VDR(n) messenger RNA (mRNA) and protein levels. PMID: 12717384
    34. Reduced expression of p21 and p27, being VDR dependent, is major pathogenic factor for nodular parathyroid gland growth in advanced secondary hyperparathyroidism. Review. PMID: 12771291
    35. Data show that in osteoblastic cells, the vitamin D receptor interacts directly with Runx2, and that this interaction contributes significantly to vitamin D3-dependent enhancement of the osteocalcin promoter. PMID: 15456860
    36. In vaginal epithelium, the presence of VDR was shown. During the estrous cycle, VDR has an important role in the proliferation and differentiation of vaginal squamous epithelium that is similar to the effects of estrogen. PMID: 15589866
    37. Binding of the ligands to ligand binding domain of rat VDR results in a shift of both Trp H(epsilon1) and N(epsilon1) resonances to lower frequencies PMID: 16130132
    38. ASBT gene expression is activated by 1,25(OH)(2)D(3) by specific binding to the VDRE and that such activation enhances ileal bile acid transport. PMID: 16481392
    39. Our results suggest that vitamin D3 may play a role in mechanisms relevant to protective properties against the neurotoxicity of glutamate through upregulation of VDR expression in cultured rat cortical neurons. PMID: 16521124
    40. These findings suggest that an unknown serum factor modulates the transactivation function of the vitamin D receptor. PMID: 16835013
    41. Expression of the classic vitamin D3 receptor in osteoblasts is required to generate a rapid 1alpha,25-dihydroxy vitamin D3-mediated increase in the intracellular Ca(2+) concentration. PMID: 16927375
    42. We also confirmed the ability of VDR to repress LXRalpha transcriptional activation using a synthetic LXRalpha responsive reporter. PMID: 17054913
    43. Depleted uranium affects both vitamin D active form and receptor expression and consequently could modulate the expression of cyp24a1 and vitamin D target genes. PMID: 17118558
    44. Lithocholic acid can induce its own catabolism through the Vdr. PMID: 17535892
    45. In the presence of a full-length side chain, the 20S configuration improves binding of specific proteins to the VDR transcriptional complex while modifications at carbon 2 do not. PMID: 17658451
    46. These results support a model where VDR preferentially recruits SRC-1 to enhance bone-specific OC gene transcription. PMID: 17786964
    47. 1,25(OH)(2)D(3) signal transduction in heart cells provides further evidence that the VDR plays a role in heart structure and function PMID: 17974622
    48. Intestinal vitamin D receptor protein levels and 1alpha,25(OH)(2)D(3) binding were diminished with ageing. PMID: 18060514
    49. A chronic exposure to enriched uranium affects both mRNA and protein expressions of renal nuclear receptors involved in vitamin D metabolism, without any modification of the circulating vitamin D. PMID: 18502116
    50. These data suggest the vitamin D receptor may play a role in the pilocarpine-induced epilepsy. PMID: 18534255

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 组织特异性：
    Detected in intestine and kidney.
  • 数据库链接：

    KEGG: rno:24873

    STRING: 10116.ENSRNOP00000011601

    UniGene: Rn.10911