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  4. Recombinant Rat Tyrosine-protein phosphatase non-receptor type 1 (Ptpn1)

Recombinant Rat Tyrosine-protein phosphatase non-receptor type 1 (Ptpn1)

  • 货号：
    CSB-YP019024RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019024RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019024RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019024RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019024RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ptpn1; Tyrosine-protein phosphatase non-receptor type 1; EC 3.1.3.48; Protein-tyrosine phosphatase 1B; PTP-1B
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-432
  • 氨基酸序列
    MEMEKEFEQI DKAGNWAAIY QDIRHEASDF PCRIAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRIMEKGSLK CAQYWPQKEE KEMVFDDTNL KLTLISEDVK SYYTVRQLEL ENLATQEARE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP IVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRRFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHVPPPP RPPKRTLEPH NGKCKELFSN HQWVSEESCE DEDILAREES RAPSIAVHSM SSMSQDTEVR KRMVGGGLQS AQASVPTEEE LSPTEEEQKA HRPVHWKPFL VNVCMATALA TGAYLCYRVC FH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
  • 基因功能参考文献：
    1. These findings reveal a novel role for pancreatic PTP1B in cerulein- and arginine-induced acute pancreatitis. PMID: 27461362
    2. deleterious role that the amygdalar PTP1B has on energy homeostasis in obesity states PMID: 28403933
    3. The results suggest that neonatal MS experience may blunt the anorectic efficacy of leptin later in life, possibly in relation with increased expressions of PTP1B and/or pSTAT3 in the hypothalamus PMID: 28229972
    4. overexpression of PTP1B can inhibit glucose-stimulated insulin secretion in INS-1 cells through negatively regulating insulin signaling. PMID: 26099503
    5. PTP1B expression is up-regulated following spinal cord injury. PMID: 25894283
    6. Study suggested that brain mitochondrial dysfunction following lipopolysaccharide-induced sepsis in rats is partly attributed to PTP1B and Src mediated decrease in mitochondrial protein tyrosine phosphorylation PMID: 25998537
    7. After retinal detachment, weak immunolabeling of PTP1B was seen in the retinal pigment epithelial cells. PMID: 25999679
    8. Overexpression of PTP1B by treatment with free fatty acids did not translate into negative modulation of insulin signaling in cells treated with unsaturated free fatty acids. PMID: 23481236
    9. Complement-mediated glomerular injury is reduced by inhibition of protein-tyrosine phosphatase 1B. PMID: 25056348
    10. An increased PTP1B expression in the hypothalamus underlies the development of leptin resistance during repeated exposure to Lipopolysaccharides. PMID: 25352433
    11. Data indicate that protein tyrosine phosphatases SHP-1, SHP-2 and PTP1B overexpressed during the development of cerulein -induced acute pancreatitis can be modulated by extracellular signal-regulated kinases (ERK1/2) and c-Jun NH2-terminal kinase (JNK). PMID: 24225419
    12. Contents released from injured epithelial cells can inactivate renal fibroblasts by dephosphorylation of EGFR through a mechanism involved in PTP1B activation. PMID: 23283996
    13. role of PTP1B in structural plasticity within the hippocampus PMID: 22844492
    14. Fe(2+)-generated O(2) mediates p21(ras) and TAK1 activation via PTP inhibition and Lys(63)-polyUb of TRAF6 in caveosomes for proinflammatory M1 activation in hepatic macrophages PMID: 22829592
    15. PTP1b within the solitary tract nucleus may be a novel molecular mechanism for preservation of resting baroreflex function and provides further evidence for deleterious cardiovascular effects associated with PTP1b inhibition PMID: 22569287
    16. The data suggest that TNFalpha acts on the hypothalamus to increase hypothalamic PTP1B expression and activity via the NFkappaB pathway PMID: 22166493
    17. The regulatory function of Src and its modulation by PTP1B on oxidative phosphorylation in mitochondria. PMID: 21063895
    18. the upregulation of PTP1B by platelet-derived growth factor or nitric oxide is both necessary and sufficient to induce the downregulation of protein kinase G via an effect on PKG mRNA levels. PMID: 21057040
    19. results indicate that activation of PTP1B in the liver could induce hypertriglyceridaemia and that anchoring of PTP1B to the membrane is crucial for its action PMID: 19605464
    20. results demonstrated that the increase in protein tyrosine phosphatase 1B expression and/or association with insulin receptor in monosodium glutamate animals may contribute to the impaired insulin signaling mainly in liver and muscle PMID: 12850498
    21. PTP1B, best known as a negative regulator of the metabolic effects of insulin, is required for the effects of insulin on actin organization in immortalized fibroblasts PMID: 12902327
    22. Protein-tyrosine phosphatase 1B is a new activator for hepatic lipogenesis via sterol regulatory element-binding protein-1 gene expression PMID: 12941932
    23. Vascular injury induced by balloon catheter increases levels. These effects occur in PTP specific, as well as temporally and regionally specific, manner. PMID: 15271661
    24. existence of an inhibitory feedback loop involving PDGF or FGF, and PTP1B in blood vessels PMID: 16373608
    25. The catalytic domain of rat recombinant protein tyrosine phosphatase eta has been produced in Escherichia coli in soluble form, crystallized, and purified to homogeneity. PMID: 16946481
    26. Diet-induced obesity increased expression and activity of the protein tyrosine phosphatase 1B and attenuated insulin signalling in gastrocnemius muscle of rats, which was reversed by single session of exercise. PMID: 17008371
    27. increases in PTP1B contribute to hypothalamic leptin resistance as rats transition into middle age PMID: 17038557
    28. PTP1B plays a role in the negative regulation of EGF-induced signaling and helps suppress cell cycle entry. PMID: 17563729
    29. PTP1B overexpression in multiple tissues in obesity is regulated by inflammation PMID: 18281274
    30. Chronic insulin treatment amplifies PDGF-BB induced motility in differentiated aortic smooth muscle cells by suppressing the expression and function of PTP1B. PMID: 18456732
    31. Somatostatin receptors 1, 2, and 5 cooperate in the somatostatin inhibition of C6 glioma cell proliferation in vitro via a phosphotyrosine phosphatase-eta-dependent inhibition of extracellularly regulated kinase-1/2. PMID: 18566118
    32. Localization of PTP-1B, SHP-2, and Src exclusively in rat brain mitochondria and functional consequences.( PMID: 18583343

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class 1 subfamily
  • 组织特异性：
    Found in several tissues including central nervous system, liver and kidney. A high level of expression was found in the hippocampus.
  • 数据库链接：

    KEGG: rno:24697

    STRING: 10116.ENSRNOP00000014309

    UniGene: Rn.11317