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  4. Recombinant Rat Transforming protein RhoA (Rhoa)

Recombinant Rat Transforming protein RhoA (Rhoa)

  • 货号：
    CSB-YP019682RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019682RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019682RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019682RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019682RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rhoa; Arha; Arha2; Transforming protein RhoA; EC 3.6.5.2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-190
  • 氨基酸序列
    MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis. Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center. May be an activator of PLCE1. In neurons, involved in the inhibiton of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets.
  • 基因功能参考文献：
    1. Ang may enhance EPC neovascular-related functions through activating RhoA/ ROCK and MAPK signaling pathway. PMID: 29991018
    2. Mevalonate pathway inhibition by rosuvastatin and alendronate prevents cardiac remodeling and dysfunction possibly through RhoA-independent mechanisms. PMID: 28665545
    3. Rhoa has a critical role in Schwann cell biology and is essential for myelination. PMID: 26820678
    4. SCYL1BP1 participated in Schwann cell (SC) differentiation and neurite outgrowth in the sciatic nerve after crush by regulating the RhoA pathway. PMID: 26572638
    5. This study showed that RhoA were increased in the same surviving neurons in the spinal cord following spinal cord injury. PMID: 27893610
    6. Femoral arteries depolarized for long periods of time (more than 1 h), exhibit sustained contraction, sensitive to cyclopiazonic acid and low concentration of nifedipine, involving Ca2+-dependent activation of the RhoA/ROCK sensitization pathway. PMID: 28526517
    7. The effects of Salmonella SseJ on kidney epithelial cell microtubules is mediated via its interactions with the RhoA. PMID: 28235057
    8. rapamycin inhibits the RhoA, Rac1, and Cdc42 activated by high glucose(HG). Thus, rapamycin shows an obvious protective effect on HG-induced Epithelial-mesenchymal transition, by inhibiting the activation of Rho GTPases (RhoA, Rac1, and Cdc42). PMID: 27093550
    9. Src inactivation decreased the p190Rho GTPase activating protein phosphorylation, promoting RhoA activation, cofilin, and myosin light chain II phosphorylation and, consequently, neurite shortening PMID: 27842221
    10. Results indicate that chronic treatment with alendronate decreases the development of cardiac and aortic remodeling, by a pathway which involves inhibition of the geranylgeranylation and activation of RhoA. PMID: 27336844
    11. Results indicated that Tau produced strong plasma membrane blebbing and F-Actin cytoskeleton remodeling by indirect activation of the GEF-H1-RhoA-ROCK pathway. All of these actions were initiated by abnormal, induced microtubule bundling and cytoplasmic release of GEF-H1. PMID: 27003208
    12. RhoA/Rho-kinase-mediated pathway is involved in the pathogenesis of PH in the nitrofen-induced CDH rat model. PMID: 25783350
    13. RhoA and Rac1 are critical to the pressure-induced proliferation and differentiation, the stress fiber assembly, and MAPK activation in mesenchymal stem cells. PMID: 25794483
    14. these observations indicate that increased fibrogenic activity because of dysregulated RhoA GTPase activity in the trabecular AH outflow pathway increases intraocular pressure in a Rho kinase-dependent manner. PMID: 25499974
    15. Data indicate that, after spinal cord injury (SCI), the small GTPase RhoA expression exhibited different patterns with different nerve cells of spinal cord. PMID: 25318874
    16. results support the concept that in a growth non-permissive environment, the regenerative capacity of sensory and motor axons is differentially affected by the RhoA/ROCK pathway PMID: 25261755
    17. Membrane ERalpha attenuates myocardial fibrosis via RhoA/ROCK-mediated actin remodeling in ovariectomized female infarcted rats. PMID: 24292258
    18. Data indicate that chronic dietary menthol blunted mesenteric arterial constriction and lowered blood pressure in genetic hypertensive rats via inhibition of RhoA/Rho kinase expression and activity in the vivo study. PMID: 24637663
    19. It was conclude that an increase in oxidative stress plays an important role in aging-associated decrease in IAS tone in the elderly and may be one of the underlying mechanisms of rectoanal incontinence in certain aging patients. PMID: 24742984
    20. Rac1 and RhoA are involved in aberrant mossy fiber sprouting . PMID: 24660528
    21. Impairment of synaptic plasticity by the stress mediator corticotropin-releasing hormone involves selective destruction of thin dendritic spines via RhoA signaling PMID: 22411227
    22. Rnh1-mediated RhoA inactivation enhances the differentiation and myelination in oligodendrocytes. PMID: 23624614
    23. a novel cardiac role of Dysbindin in the activation of RhoA-SRF and MEK1-ERK1 signaling pathways and in the induction of cardiac hypertrophy, is reported. PMID: 24385487
    24. Spontaneous RhoA activity at membrane protrusions is spatially associated with closure, but not formation of inter-endothelial gaps, whereas RhoA activity at distant contractile filaments contributes to thrombin-induced disruption of junctional integrity. PMID: 23536606
    25. alcohol-induced inhibition of mesenteric lymphatic myogenic constriction is mediated by reduced RhoA/ROCK-mediated Ca(2+) sensitivity. PMID: 23237297
    26. Our results suggest that the small GTPase RhoA, but not Rac1, in the dorsal hippocampus is responsible for conditioned place aversion formation, mainly through its regulation of actin rearrangements PMID: 23564082
    27. In response to stimulation, the collapse of the leading cell migration front requires loss of F-actin and focal adhesion, whereas the subsequent reversal of soma translocation depends on RhoA-myosin signaling pathways. PMID: 23361995
    28. Data indicate that atg7, RhoA and hnRNP-Q1 are involved in autophagy negatively regulates early axon growth in cortical neurons. PMID: 23918799
    29. TcdA altered intestinal cell morphology and cytoskeleton organization, decreased cell proliferation, and increased cell apoptosis. Ala-Gln and Gln supplementation reduced intestinal epithelial cell damage and increased RhoA expression. PMID: 23484083
    30. increased RhoA activity could compensate vascular hyporeactivity in early endotoxaemia PMID: 23457552
    31. While PKC and RhoA/ROCK pathways play a significant role in slow-rate high-amplitude spontaneous phasic activity, only the RhoA/ROCK pathway primarily mediates fast-rate low-amplitude phasic activity, in the rectal smooth muscle. PMID: 23413252
    32. Evaluated the effect of Sal B on portal hypertension and on hepatic stellate cells contractility. Sal B decreased ET-1-induced RhoA and Rho-associated coiled coil-forming protein kinase (ROCK) II activation by 66.84% and by 76.79%, respectively. PMID: 22986787
    33. RhoA/ROCK signaling plays a role in endothelial-monocyte-activating polypeptide (EMAP) II-induced increase in blood-tumor barrier (BTB) permeability in rat brain microvascular endothelial cells. PMID: 21647708
    34. analysis of regulation of RhoA signaling by the cAMP-dependent phosphorylation of RhoGDIalpha PMID: 23012358
    35. alpha-crystallin counteracts the inhibitory effect of myelin inhibitory factors through regulation of RhoA/Rock signaling pathway. PMID: 21584655
    36. In the diabetic heart, the RhoA/ROCK pathway contributes to contractile dysfunction at least in part by sustaining PKCbeta(2) activation and reactive oxygen species production via a positive feedback loop that requires an intact cytoskeleton. PMID: 22865386
    37. Initial actin coating of fused vesicles is dependent on activation of Rho and formin-dependent actin nucleation. Actin coat contraction is facilitated by myosin II. PMID: 22427691
    38. Transforming growth factor beta1 induced cytoskeletal remodelling and migration of rat hepatic stellate cells following RhoA activation. The level of RhoA activation determined the cell motility. PMID: 22498718
    39. Results establish RhoA as an important regulatory protein in the hippocampal stem cell niche. PMID: 21956892
    40. A mechanism is suggested whereby ethanol may disrupt neurotrophic factor BDNF regulation of axon growth and guidance via inhibition of hippocampal growth cone signaling. PMID: 21676004
    41. Local disruption of these active RhoA distributions led to the morphological change from the flattened into process-bearing shape and promoted process outgrowth. PMID: 21946234
    42. Intermedin increases permeability of rat coronary microvascular endothelial cells via RhoA/Rac1 dependent derangement of the actin cytoskeleton. PMID: 21816966
    43. RhoA is involved in simvastatin-mediated membrane trafficking of AQP2. PMID: 21511701
    44. Stretch-induced activation of Rac1 and RhoA differentially regulates angiogensinogen gene expression by modulating p38 and JNK activation in cardiac fibroblasts. PMID: 21131638
    45. Geraniol can induce apoptosis and inhibit RhoA activation during rat hepatocarcinogenesis promotion. PMID: 20435455
    46. ET-1 augments O-GlcNAcylation and this modification contributes to increased vascular contractile responses via activation of the RhoA/Rho-kinase pathway PMID: 20978008
    47. This study showed that adiponectin mediates actin cytoskeleton remodeling to translocate lipoprotein lipase and allow subsequent activation. PMID: 21147877
    48. in glomerular mesangial cells, caveolae are important mediators of strain-induced ROS generation through NADPH oxidase, mediating a signaling cascade which results in RhoA activation PMID: 20380579
    49. Treatment of urethral smooth muscle cells with estrogen, raloxifene or levormeloxifene resulted in decreased expression of RhoA in a dosage-dependent manner. PMID: 20970835
    50. Data suggest that RhoA activation is important for bradykinin-induced increase in blood-tumor barrier permeability and appears to involve the ability for RhoA to mediate occludin disassembly and stress fiber formation. PMID: 20369389

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex. Midbody. Cell projection, lamellipodium. Cell projection, dendrite.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 数据库链接：

    KEGG: rno:117273

    STRING: 10116.ENSRNOP00000066672

    UniGene: Rn.107401