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  4. Recombinant Rat Thimet oligopeptidase (Thop1)

Recombinant Rat Thimet oligopeptidase (Thop1)

  • 货号：
    CSB-YP023508RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023508RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023508RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023508RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023508RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Thop1; Thimet oligopeptidase; EC 3.4.24.15; Endo-oligopeptidase A; Endopeptidase 24.15; PZ-peptidase; Soluble metallo-endopeptidase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-687
  • 氨基酸序列
    MKPPAACAGD VVDTVSPCST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR VGAQDFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP NKDIRAASTE ADKKLSEFDV EMSMRQDVYQ RVVWLQEKIP KDSLKPEAAR YLERLIKLGR RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK NLNEDTTFLP FTREELGGLP EDFLNSLEKT EDGKLKVTLK YPHYFPLLKK CHVPETRRLL EEAFNCRCKE ENCAILKELV SLRAQKSNLL GFRTHADYVL EMNMAKTSQT VATFLDELAR KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR VDQNLLKEYF PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR DAASGEEIGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQLAIAAMVA NFTKPTPDVP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEKEPLMR MSQHYRTGGE APEDLLEKLI KSRQANAGLF NLRQIVLAKV DQVLHTQTDV DPAEEYARLC QEILGVPATP GTNMPATFGH LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG SEDASTMLKQ FLGRDPKQDA FLLSKGLQVE GCEPPAC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation.
  • 基因功能参考文献：
    1. Coincubation of Kiss1 and EP24.15 demonstrated multiple cleavages of the peptide in vitro. immunohistochemistry indicated coexpression of EP24.15 and Kiss1, implicating that the regulation of Kiss1 by EP24.15 could occur in vivo. PMID: 26653570
    2. we show that Ang-(1-7) is the main metabolite of angiotensin I in rat hippocampi, and, strikingly, that thimet oligopeptidase is the main enzyme involved in the generation of Ang-(1-7). PMID: 24041943
    3. TOP changes in the brain could play important role in the balance of neuropeptide level correlated with cocaine effects. PMID: 22387539
    4. Data report mapping of the positions of the 224 surface residues that differ in sequence between thimet oligopeptidase and neurolysin onto the surface of the neurolysin crystal structure. PMID: 12192079
    5. this is the first evidence of an in vivo role for EP 24.15 in brain function, specifically in regulating alterations in microvessel permeability induced by exogenous bradykinin PMID: 12586639
    6. Analysis of transcription factors binding to promoter consensus sequences of the thimet oligopeptidase gene. Gene expression is differentially regulated in neuroendocrine and spermatid cell lines. PMID: 12911328
    7. data suggest that calcium may be an important modulator of ep24.15 cell function PMID: 15955058
    8. PTZ-induced seizures of the rats activates NMDA receptors, then decrease thimet oligopeptidase enzyme activity and immunoreactivity in hippocampal neurons. PMID: 15985312
    9. together, these data demonstrate that EP24.15/16 modulate intracellular, peptidergic signaling cascades through B2R in a physiologically relevant nociceptive system. PMID: 16515556
    10. increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load PMID: 18571100

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase M3 family
  • 组织特异性：
    Expressed abundantly in the testis. It is also found in the liver, lung and kidney.
  • 数据库链接：

    KEGG: rno:64517

    STRING: 10116.ENSRNOP00000027045

    UniGene: Rn.9490