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  4. Recombinant Rat Synaptosomal-associated protein 25 (Snap25)

Recombinant Rat Synaptosomal-associated protein 25 (Snap25)

  • 货号：
    CSB-YP021873RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021873RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021873RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021873RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021873RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Snap25; SnapSynaptosomal-associated protein 25; SNAP-25; Super protein; SUP; Synaptosomal-associated 25 kDa protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-206
  • 氨基酸序列
    MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
  • 基因功能参考文献：
    1. This study reveals a critical role for the axonal synthesis of SNAP25 in the assembly of presynaptic terminals. PMID: 28954226
    2. analysis of crystal structures and biochemical analyses of Rabphilin-3A C2B-SNAP25 and C2B-phosphatidylinositol 4,5-bisphosphate (PIP2) complexes, revealing how Rabphilin-3A C2 domains operate in cooperation with PIP2/Ca(2+) and SNAP25 to bind the plasma membrane, adopting a conformation compatible to interact with the complete SNARE complex PMID: 28634303
    3. Our study uncovers the postsynaptic function of the SNAP25-VAMP1-syntaxin4 complex in mediating the constitutive exocytosis of NMDA receptors, suggesting that this SNARE complex is involved in excitatory synaptic transmission PMID: 27791016
    4. Findings indicate that lower SNAP-25 in ventromedial caudate in schizophrenia is primarily due to less SNAP-25A, and that the remaining SNAP-25 is associated with greater protein-protein interaction with syntaxin. The absence of a link to recent treatment effects in the human samples, or to administration of antipsychotic drugs in rats suggests the findings are illness- and not treatment-related. PMID: 26971072
    5. Electrostatic anchoring precedes stable membrane attachment of SNAP25 and SNAP23 to the plasma membrane. PMID: 28240595
    6. Data suggest that GTP-binding protein beta/gamma (Gb/g) heterodimers interact specifically with lipid-embedded neuronal t-SNARE proteins syntaxin 1A and SNAP-25B (synaptosomal-associated protein 25B); this binding is competitive with synaptotagmin 1 for binding sites on the t-SNARE proteins; Gb/g inhibits Ca2+/synaptotagmin 1-dependent membrane fusion. PMID: 28515322
    7. Our results will provide novel evidence to reveal the possible role of SNAP-25 in B[a]P-induced neurotoxicity and may be helpful for searching the potential strategy for the prevention measures against B[a]P neurotoxicity. PMID: 28412278
    8. directions of changes in the SNAP-25 protein levels in cerebellar granule cells induced by tetrabromobisphenol-A, thalidomide and valproic acid were consistent with the direction of changes in the mRNA level PMID: 27693314
    9. TNFalpha induces co-trafficking of TRPV1/TRPA1 in VAMP1-containing vesicles to the plasmalemma via Munc18-1/syntaxin1/SNAP-25 mediated fusion. PMID: 26888187
    10. Data demonstrate that the open-closed syntaxin equilibrium is shifted toward the open state when syntaxin and Munc18-1 are associated with SNAP25, and the results indicate that a syntaxin/SNAP25:Munc18-1 complex is a likely starting point for SNARE assembly. PMID: 26876096
    11. The SNAP-25 in the insular cortex as a part of pathophysiological process that determines predisposition of Krushinsky-Molodkina rats to audiogenic seizures. PMID: 26923581
    12. SNAP25 plays a crucial role in sensory functional recovery following spinal cord injury PMID: 24519330
    13. protein is mostly important in tissues outside the brain. Our developmental data showed a low expression of Snap25 during fetal life with a sharp increase in the expression of Snap25 just after birth, between postnatal day 2 and 6. PMID: 23135794
    14. SNAP-25 facilitated vesicle mobilization to the readily releasable pool, most likely via their roles in endocytosis and/or exocytosis. PMID: 23643538
    15. Reduced SNAP-25 alters short-term plasticity at developing glutamatergic synapses. PMID: 23732542
    16. The results of this study provided the first evidence showing the dual role of SNAP25 and synaptobrevin in both exocytosis and slow endocytosis at conventional synapses. PMID: 23699527
    17. syntaxin 1 and SNAP-25 cooperate as SNARE proteins to support neuron survival. PMID: 23403573
    18. These results provide direct experimental support for the availability, in a subpopulation of SNAP-25, of vicinal thiols that may confer on one or more isoforms of this family of proteins a sensitivity to oxidative stress. PMID: 21850520
    19. PRIP is involved in the regulation of the phospho-states of SNAP-25 by modulating the activity of PP1, thus regulating exocytosis. PMID: 22311984
    20. AC6-mediated inhibition of neurite outgrowth was reversed by the overexpression of Snap25 or a Snapin mutant that could not be phosphorylated. PMID: 21986494
    21. endogenous SNAP-25 plays an important role in P/Q-type channel regulation in glutamatergic neurons. PMID: 21558797
    22. Letter: incubation of pancreatic islets with fibronectin improves beta cell function via increased expression of syntaxin 1 and SNAP25. PMID: 21926557
    23. Data show that the C-terminus of SNAP-25 is a binding target for Gbetagamma necessary for both transient transmitter-mediated presynaptic inhibition, and the induction of presynaptic LTD. PMID: 21633701
    24. Palmitoylation determines the precise intracellular distribution of SNAP25. PMID: 21429935
    25. SNAP25 was upregulated in frontal cortex but downregulated in caudate putamen. PMID: 20945070
    26. There was a more than 10-fold excess of SNAP-25 over syntaxin-1. PMID: 21076040
    27. In the incisor dental pulp, all nerve fibers display immunoreactivity for syntaxin-1, SNAP-25, and vesicle-associated membrane protein (VAMP)-2. PMID: 20186959
    28. endogenous SNAP-25 regulates native voltage-gated calcium channels in glutamatergic neurons PMID: 20522554
    29. SNAP23 and SNAP25 palmitoylation is regulated by DHHC palmitoyl transferases PMID: 20519516
    30. Synaptobrevin N-terminally bound to syntaxin-SNAP-25 defines the primed vesicle state in regulated exocytosis. PMID: 20142423
    31. This study suggested that overactivity of SNAP-25 in late adolescent-adult brain reduces the signal-to-noise ratio and potential for synaptic plasticity within circuitry controlling spatial and fear memory consolidation resulting in cognitive deficits. PMID: 20002519
    32. In conclusion, the preferential localization of SNAP25 in specific synaptic boutons suggests a correlation between SNAP25 and the Pr. This evidence supports the hypothesis that SNAP25 has a modulatory role in shaping synaptic responses. PMID: 19735702
    33. binding and inhibition of delayed rectivier potassium channels in secretory cells PMID: 11925439
    34. although SNAP-25 phosphorylation at Ser(187) occurs in insulin-secreting cells and is mediated by PKC, it does not appear to play a major role in regulated insulin secretion. PMID: 12164783
    35. Hypophysectomy increased adrenal SNAP-25. Corticosterone did not change levels of SNAP-25, but thyroid hormone decreased adrenal SNAP-25, demonstrating the potential importance of the pituitary-thyroid axis. PMID: 12165670
    36. Expression of SNAP-25 and syntaxin-1 in adenohypophyses of hypothyroidism. Thyroidectomy changes expression and immunoreactivity of SNAP-25 and syntaxin-1 in adenohypophysis. These effects can be reversed by T4 administration. PMID: 12398231
    37. a SNAP-25 mutant can reconstitute exocytosis in BoNT/E-treated cells [review] PMID: 12438121
    38. synaptosome-associated protein of 25 kDa and vesicle-associated membrane protein are involved in secretion of polypeptides from the choroid plexus epithelium. PMID: 12559091
    39. the syntaxin/SNAP-25 dimer binding to synaptotagmins I and II is mediated by an evolutionarily conserved motif PMID: 14709554
    40. Exogenous expression of SNAP-25 in GABAergic interneurons lowered calcium responsiveness, and SNAP-25 silencing in glutamatergic neurons increased calcium elevations evoked by depolarization. PMID: 14980208
    41. chronic morphine treatment inhibits phosphorylation of SNAP-25 at Ser187 and leads to a down-regulation of SNARE complex formation PMID: 15277518
    42. the results of this study suggest that SNAP-25 in the CA1 region is involved in memory consolidation. PMID: 15355326
    43. Results suggest that SNAP-25 protein is involved in axon outgrowth and synaptogenesis in the nervous system. PMID: 15478103
    44. different raft-targeting signals within SNAP-25 and SNAP-23 are likely important for fine-tuning the exocytic pathways in which these proteins operate PMID: 15542596
    45. Correlation between the number of SNAP-25 clusters and the number of docked insulin granules, which is associated with the fusion of insulin granules. PMID: 15647897
    46. However, effects of diazoxide on SNAP-25 protein were nullified by proteasome inhibitors (ALLN, MG-132, and epoxomicin) but not by lysosomal inhibition (NH(4)Cl). PMID: 15752769
    47. The binding of multiple syntaxins to SNAP-25 in PC12 cells is reported. PMID: 15975093
    48. These results indicate that phospholipase A(2)-IIA is coexpressed with SNAP-25 in mature taste receptor cells that possess exocytotic machinery. PMID: 16385482
    49. We conclude that different domains within SNAP-25 can form distinct complexes with Kv2.1 to execute a fine allosteric regulation of channel gating and the architecture of the outer pore structure in order to modulate cell excitability. PMID: 16478442
    50. Treatment with botulinum neurotoxins A and B strongly reduced somatodendritic dopamine release, thus demonstrating the requirement for SNARE proteins SNAP-25 and synaptobrevin. PMID: 16539689

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  • 亚细胞定位：
    Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Photoreceptor inner segment.
  • 蛋白家族：
    SNAP-25 family
  • 数据库链接：

    KEGG: rno:25012

    UniGene: Rn.107689