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  4. Recombinant Rat Secretin (Sct)

Recombinant Rat Secretin (Sct)

  • 货号：
    CSB-YP020864RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP020864RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP020864RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP020864RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP020864RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sct
  • Uniprot No.：
  • 别名：
    Sct; Secretin
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    33-59
  • 氨基酸序列
    HSDGTFTS ELSRLQDSAR LQRLLQGLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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靶点详情

  • 功能：
    Hormone involved in different processes, such as regulation of the pH of the duodenal content, food intake and water homeostasis. Exerts its biological effects by binding to secretin receptor (SCTR), a G-protein coupled receptor expressed in the basolateral domain of several cells. Acts as a key gastrointestinal hormone by regulating the pH of the duodenal content. Secreted by S cells of the duodenum in the crypts of Lieberkuehn and regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas. Production of bicarbonate is essential to neutralize the pH and ensure no damage is done to the small intestine by the gastric acid. In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation. Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation. Also able to stimulate lipolysis in white adipocytes. Also plays an important role in cellular osmoregulation: released into the systemic circulation in response to hyperosmolality and acts at different levels in the hypothalamus, pituitary and kidney to regulate water homeostasis. Also plays a role in the central nervous system, possibly by acting as a neuropeptide hormone: required for hippocampal synaptic function and neural progenitor cells maintenance.
  • 基因功能参考文献：
    1. propose that eyeblink conditioning (EBC)-induced secretin release from Purkinje cells modulates EBC acquisition by reducing surface expression of Kv1.2 at one or both of these sites PMID: 25403455
    2. Secretin decreases cell surface Kv1.2 alpha-subunit levels by modulating Kv1.2 alpha- subunit endocytic trafficking. PMID: 22764231
    3. Ursodeoxycholic acid is conjugated in order to promote secretin-stimulated hydrocholeresis in rats through Ae2, microtubules, intracellular Ca(2+), PKCalpha, PI3K, PKA, and MEK. PMID: 22194894
    4. Molecular basis of secretin docking to its intact receptor using multiple photolabile probes distributed throughout the pharmacophore. PMID: 21566140
    5. Data show that in secretin/secretin receptor binding, the alpha-helix in natural secretin was oriented approximately parallel to the amino-terminal alpha-helix of the receptor. PMID: 21388146
    6. Review. In the rat cerebellum, secretin functions as a retrograde messenger to facilitate GABA transmission, indicating that it can modulate motor and other functions. PMID: 12392059
    7. first immunohistochemical demonstration of secretin-immunoreactive elements in the brain stem and in primary sensory ganglia PMID: 12409229
    8. Secretin mRNA is present in gastric mucosa and the locally released secretin may exert a paracrine effect to inhibit acid secretion. PMID: 12609767
    9. role in activating chromogranin A transcription PMID: 12646581
    10. solitary nucleus neurons are activated by secretin as a consequence of activation of a non-selective cation conductance and support the emerging view that secretin can act as a neuropeptide within the CNS. PMID: 14715495
    11. COlitis in ganciclovir-treated Sec-simplex virus thymidine kinase mice arises from depletion of secretin gene-expressing lymphoid cells. PMID: 15337838
    12. secretin stimulates cholehepatic shunting of conjugated bile acids and is associated with increased cholangiocyte apical membrane ASBT PMID: 15834929
    13. Solid evidence for the role of secretin as a neuropeptide in the mammalian central nervous system. PMID: 15963647
    14. provide evidence of the endogenous release of secretin in the hypothalamus and the mechanisms underlying this release PMID: 16888165
    15. Atrial natriuretic factor may play a protective role by reducing secretin response to avoid overstimulation. PMID: 16973919
    16. propose that secretin works at multiple levels in the hypothalamus, pituitary, and kidney to regulate water homeostasis PMID: 19805236

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glucagon family
  • 组织特异性：
    In the brain, expressed in the central amygdala, hippocampus, area postrema, nucleus of the tractus solitary and cerebellum. Expressed at higher level in the cerebellum.
  • 数据库链接：

    KEGG: rno:24769

    STRING: 10116.ENSRNOP00000024094

    UniGene: Rn.9919