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  4. Recombinant Rat Retinoblastoma-associated protein (Rb1), partial

Recombinant Rat Retinoblastoma-associated protein (Rb1), partial

  • 货号：
    CSB-RP155294r(A3)
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rb1; Rb-1; Retinoblastoma-associated protein; pRb; Rb; pp105
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    26.6kDa
  • 表达区域：
    721-919aa
  • 氨基酸序列
    KDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGSDEADGSKHLPAESKFQQKLAEMTSTRTRMQKQKLNDSMEISNKEE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes. Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription. Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase. RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex.
  • 基因功能参考文献：
    1. Identify Dyrk1a as a novel negative regulator of D-cyclin-mediated Rb1/E2f-signalling. As dysregulation of this pathway with impaired cardiomyocyte proliferation leads to cardiomyopathy. PMID: 27056896
    2. Inflammation disrupts LDLr feedback regulation through the activation of the mTOR pathway, increasing mTORC1 activity and up-regulating SREBP-2-mediated cholesterol uptake through Rb phosphorylation. PMID: 24068000
    3. Phosphorylated retinoblastoma protein (p-Rb) is involved in neuronal apoptosis after traumatic brain injury in adult rats. PMID: 23371354
    4. a role for Rb protein in directing oncogenic cell growth PMID: 22885065
    5. Rb pathway may only be involved in early neuronal apoptosis and may be not an apoptotic signal in the late stages of transient cerebral ischemia. PMID: 19123049
    6. Results indicate that HIV-infection in the brain is associated with site-specific hyperphosphorylation of pRb at serine795, and that this phosphorylation contributes to neuronal death in this disease. PMID: 21504794
    7. Data show that proliferaton and nuclear expression of cyclin D1 and p-Rb coincide with the early phase of cyst growth, suggesting that there might be a therapeutic window in which cyclin-dependent kinase inhibitors are most effective in ARPKD. PMID: 20924203
    8. proliferative lesions activate the cell cycle machinery following tumor promotion via a regulatory mechanism involving inactivation of GSK3beta and retinoblastoma protein PMID: 19505811
    9. These data demonstrate how a new molecular network on ATM regulates the cell cycle through the control of pRb phosphorylation. PMID: 20213763
    10. Expression of Rb is closely event-related to malignant transformation and conversion of the oral mucosa, and is a reliable biomarker linked to oral cancer pathogenesis. PMID: 20023236
    11. employs both canonical cyclin-dependent kinase/cyclin regulation and metabolic regulation as a means to limit proliferation, underscoring its potency in tumor suppression PMID: 12221087
    12. Rb is present in rat and human islets, and overexpression of cyclin D(1)/cdk-4 led to strikingly enhanced Rb phosphorylation. PMID: 14693709
    13. Mitogen-induced rapid phosphorylation of serine 795 of the retinoblastoma gene product in vascular smooth muscle cells involves ERK activation PMID: 15069084
    14. MyoD may function in myonuclei in response to denervation to protect against denervation-induced apoptosis via perhaps the activation of p21 and Rb PMID: 15084472
    15. continued RB action is required for the maintenance of two kinetically and functionally distinct modes of replication inhibition PMID: 15169903
    16. RB plays a role in the early neural differentiation of mysenchymal stem cells through histone deacetylases dependent pathways. PMID: 15459751
    17. the derepression of Rb-E2F-regulated genes leads to apoptosis through inactivation of focal adhesion kinase and activation of caspase-8 PMID: 15640164
    18. Caspase-9 antagonizes p53-induced apoptosis by generating a p76(Rb) truncated form of Rb PMID: 15735701
    19. ERK1/2 mediates EGF-induced rapid Rb phosphorylation at Ser780 and Ser795 PMID: 16126730
    20. deregulation of p16-cyclin D1/Cdk4-Rb pathway, but not oncogenic activation of ras, plays a crucial role in bladder tumorigenesis induced by bladder calculi PMID: 16896691
    21. Results suggest that phosphorylation of the cell cycle regulator, Rb, contributes to death in oxygen-glucose deprivation in septal neurons but Cdk5 can have a protective role. PMID: 18351461
    22. Results show that the Rb-E2F pathway functions as a bistable switch to convert graded serum inputs into all-or-none E2F responses. PMID: 18364697
    23. Results suggest that proper CXCR4 stimulation by CXCL12 in the mature CNS can prevent impairment of the Rb-E2F pathway and support neuronal survival. PMID: 18583990
    24. Ink4a/Cdk4/Rb1 pathway deregulation, more than Arf/Mdm2/Tp53 pathway, has a major role in the development of these tumors through p16(Ink4a) deregulation. PMID: 18656278
    25. Cdk2 signaling pathways are critical regulators of cardiac I/R injury in vivo and support a cardioprotective role for Rb PMID: 18692063
    26. These results indicate that cyclin D-cdk4/6-dependent phosphorylation of pRb and activation of E2F is necessary for hypertrophic growth in cardiomyocytes. PMID: 18700867

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Retinoblastoma protein (RB) family
  • 数据库链接：

    KEGG: rno:24708

    STRING: 10116.ENSRNOP00000021752

    UniGene: Rn.55115