Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Receptor tyrosine-protein kinase erbB-4 (Erbb4), partial

Recombinant Rat Receptor tyrosine-protein kinase erbB-4 (Erbb4), partial

  • 货号：
    CSB-YP733850RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP733850RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP733850RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP733850RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP733850RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Erbb4; Tyro-2; Receptor tyrosine-protein kinase erbB-4; EC 2.7.10.1; Proto-oncogene-like protein c-ErbB-4) [Cleaved into: ERBB4 intracellular domain; 4ICD; E4ICD; s80HER4)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.
  • 基因功能参考文献：
    1. It is a member of the receptor tyrosine kinase family, plays critical roles in development and function of neural circuitry, relevant to behavioral deficits. PMID: 29674181
    2. ErbB4 plays a neuroprotective role in early brain injury after subarachnoid hemorrhage. PMID: 28756200
    3. The Paracrine Effect of Skeletal Myoblasts Is Cardioprotective Against Oxidative Stress and Involves EGFR-ErbB4 Signaling, Cystathionase, and the Unfolded Protein Response PMID: 26021843
    4. NRG-1, ErbB2, and ErbB4 expression significantly decreased in vein grafts over time. PMID: 25978692
    5. Findings reveal an intimate reciprocal relationship between ErbB4 and GluN2b NMDA receptors via NRG2/ErbB4 signaling. PMID: 26027736
    6. Exercise training and return to a well-balanced diet activate the neuregulin 1/ErbB pathway in skeletal muscle of obese rats PMID: 25820551
    7. These results indicate that ecto-ErbB4 could be used in vivo as a tool to manipulate the neuregulin1/ErbB system. PMID: 25177687
    8. Data show that each of the four ErbB4 isoforms is able to confer cells with an adhesion preference for cells expressing the transmembrane neuregulin 1 type III PMID: 24518229
    9. ErbB4 reduces synaptic GABAA currents independent of its receptor tyrosine kinase activity. PMID: 24218551
    10. neuregulin-1/ErbB signaling plays an important role in central remyelination under pathological conditions and contributes to the alleviation of pain. PMID: 23097328
    11. NMDA receptor antagonism early in life can have long term effects on neuregulin (Nrg)1/erbB4 expression which could be important in understanding pathological processes that might be involved in schizophrenia. PMID: 21962913
    12. NRG1 stimulation of the ErbB4 receptor exerts a sustained divalent calcium-ion increase in a neural progenitor cell line. PMID: 21991932
    13. A role for NRG1 and ERBB4-4ICD signaling in the regulation of gene expression contributes to normal and schizophrenic neuronal function. PMID: 21352860
    14. Thus ErbB4 localizes to the nucleus as a full-length protein, and plays a role in the DNA damage response induced by doxorubicin in cardiac myocytes. PMID: 22244893
    15. AAK1 is a relevant target of K252a, that the loss of AAK1 alters ErbB4 trafficking and expression levels, providing evidence for a previously unrecognized role for AAK1 in Nrg1-mediated neurotrophic factor signaling. PMID: 21802010
    16. Suggest that the up-regulation of miR-146a after doxorubisin (Dox) treatment is involved in acute Dox-induced cardiotoxicity by targeting ErbB4. PMID: 20495188
    17. Data showed that NRG1beta and ErbB4 expressions were significantly increased in the rat prefrontal cortex and hippocampus in rat schizophrenia model. Findings suggest that altered expressions of NRG1 and ErbB4 might be attributed to the schizophrenia. PMID: 20467458
    18. Expression of ErbB2 and ErbB4 receptor mRNA is down-regulated in myocardial infarction. PMID: 18705011
    19. ErbB4 may participate in tooth morphogenesis PMID: 12112465
    20. Early onset and preferential expression of ErbB4 in tangentially migrating interneurons suggests that neuregulin receptor ErbB4 signaling may regulate the development and function of telencephalic interneurons. PMID: 12571115
    21. Neuregulin stimulation causes translocation of ErbB4 into lipid rafts and these are necessary for signaling by ErbB4 PMID: 12716924
    22. Testicular acid phosphatase acts as a tyrosine phosphatase to modulate signals mediated by ErbB4 that are important for neuronal development and synaptic plasticity. PMID: 15219672
    23. ErbB4 signaling via PI3K activation plays a fundamental role in controlling NRG1beta1-induced migration PMID: 15355992
    24. The functional significance for cell volume regulation of the ErbB4-Src-PI3K signaling cascade is indicated by the 48-66% decrease of the hyposmotic taurine efflux observed by inhibition of these kinases. PMID: 15934939
    25. Neuregulin mediates survival of PC12-ErbB-4 cells from apoptosis induced by serum deprivation, tumor necrosis factor-alpha treatment, or hydrogen peroxide. PMID: 15968086
    26. ErbB4 receptors when activated by neuregulin play an important role in neuron survival after MPP+ treatment. This requires PI3K/PKB/Akt and Ras/MapK signaling networks. PMID: 17085783
    27. ErbB4 endocytosis plays a role in neuregulin-1 signaling in neurons. PMID: 17250808
    28. erbB4 is an important receptor in the control of fetal lung type II cell maturation PMID: 17545485
    29. Results describe the regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons. PMID: 17562386
    30. Taken together, these results show that ErbB4 is expressed by neuronal somatodendrites in cerebral cortex and hippocampus from birth to adulthood, and support a role for neuregulins in dendritic growth and plasticity. PMID: 17630218
    31. ErbB4 isoform that is proteinase cleavable but does not contain a PI3K-binding domain in its cytoplasmic tail, mediates important functions in renal epithelial cells in response to HB-EGF PMID: 17761534
    32. NRG1/ErbB4 signaling differentially regulates synapse maturation and dendritic morphology via two distinct mechanisms involving trans-synaptic signaling and tyrosine kinase activity, respectively PMID: 18819924
    33. Data show that the expression of ErbB4 mRNAs is downregulated after end-to-end and end-to-side nerve regeneration in the rat median nerve model. PMID: 18845940
    34. ventricular ErbB2 and ErbB4 receptors become activated during the development of pacing-induced heart failure, but downstream signaling is partly abrogated PMID: 19010331
    35. The s conclude that ErbB4 participates in maintaining adult lung alveolar epithelial cell surfactant synthesis and proliferation with development-specific functions. PMID: 19191103

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, EGF receptor subfamily
  • 组织特异性：
    Preferentially expressed in the developing nervous system. Exhibits distinct and highly regionalized patterns of expression in the adult brain, where it is mainly found in the reticular nucleus of the thalamus. Very low levels in kidney, and heart.
  • 数据库链接：

    KEGG: rno:59323

    STRING: 10116.ENSRNOP00000019283

    UniGene: Rn.163078