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  4. Recombinant Rat Ras-related C3 botulinum toxin substrate 1 (Rac1)

Recombinant Rat Ras-related C3 botulinum toxin substrate 1 (Rac1)

  • 货号：
    CSB-YP754338RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP754338RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP754338RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP754338RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP754338RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rac1; Ras-related C3 botulinum toxin substrate 1; EC 3.6.5.2; p21-Rac1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-189
  • 氨基酸序列
    MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity. In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX. In synapses, may mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization.
  • 基因功能参考文献：
    1. Pharmacological inhibition of RAC1 could significantly inhibit the proliferation of both RT4 cells and human NF2-associated primary schwannoma cells by inducing apoptosis. Pharmacological inhibition of RAC1 effectively reduced Rac1 activity and down-regulated the pathway downstream of Rac. Moreover, pharmacological inhibition of RAC1 showed a potential antitumor effect, with low toxicity in vivo. PMID: 28934903
    2. Decreased cardiac Rac1 expression in the nitrofen-induced congenital diaphragmatic hernia suggests that Rac1 deficiency during morphogenesis may impair structural cardiac remodeling, resulting in congenital cardiac defects. PMID: 28683507
    3. Glucotoxicity promotes aberrant activation and mislocalization of Rac1 and metabolic dysfunction in pancreatic islet beta-cells. PMID: 28828705
    4. Rac1 signaling regulates the expression of the inflammasome and plays a pivotal role in the pathogenesis of hyperoxia-induced neonatal lung injury. PMID: 28013306
    5. longest Rac1 3' UTR is necessary for driving the mRNA to the neurites, and also for neurite outgrowth in cortical neurons. PMID: 28274785
    6. miR-124 directly controls Rac1 expression. PMID: 27159442
    7. contextual fear memory in rats is actively regulated by Rac1 activity in the hippocampus. PMID: 25613020
    8. The spinal GTP-Rac1-PAK-ERK/p38MAPK signaling pathway is involved in both the development and maintenance of peripheral inflammatory pain and can be used as a potential molecular target for developing a novel therapeutic strategy for clinical pain PMID: 26700000
    9. Rac1 is an important mediator of pulmonary vascular remodeling. PMID: 26543024
    10. These findings suggest that periodic mechanical stress promotes nucleus pulpous cell proliferation in part through the EGFR-Rac1-ERK1/2 signaling pathway, which links these three important signaling molecules into a mitogenic cascade. PMID: 26707876
    11. Rac1 (and RhoA) are effectors of D1 receptor signaling during dendritic morphogenesis in prefrontal cortex. PMID: 24915967
    12. Localized in the membrane in association with Rac1 following the LPS stimulation. PMID: 25796615
    13. That is accompanied by a massive rise in Rac1-GTP level. PMID: 25957600
    14. In immunohistochemical staining, expression of Rac1 was observed to be increased in all layers of bladder in both diabetic groups compared to the control group PMID: 25382267
    15. Activation of Rac1 may not require plasma membrane localization, potentially leading to differential activation of cytosolic signaling pathways that alter cell morphology. PMID: 25479592
    16. experiments demonstrate that Rac in the basolateral amygdala is crucial for the reconsolidation of auditory Pavlovian fear memory, whereas Rac in the CA1 is critical for the reconsolidation of contextual Pavlovian fear memory PMID: 24553575
    17. Exendin-4 confers resistance to ANG II-induced superoxide anion generation from NAD(P)H oxidase and the resultant VSMC senescence by inhibiting Rac1 activation via a cAMP/PKA-dependent pathway. PMID: 25298426
    18. Fluctuations in gene expression over prolonged periods of time, and the resulting fluctuations in Rac1 activity, could generate glioma cells that have different invasive capacities. PMID: 24522191
    19. Rac1 translocation to the nucleus functionally correlates with bFGF-induced neurite outgrowth. PMID: 24195795
    20. Rac1 and RhoA are involved in aberrant mossy fiber sprouting. PMID: 24660528
    21. Taken together, these data suggest that Chlamydia pneumoniae infection promotes vascular smooth muscle cell migration, possibly through activating Rac1 via PI3K. PMID: 24248991
    22. results identify inhibition of Rac1/p21-activated kinase actin signaling pathways as an activity-dependent mechanism mediating the inhibitory effects of GluN3A on spine morphogenesis. PMID: 24297929
    23. Data from RAC1 knockdown in INS-1 cell line suggest that RAC1 plays a crucial role in insulin secretion by beta cells in response to increased glucose; RAC1 appears to act by depolymerizing actin cytoskeleton. PMID: 23412604
    24. The activation of Rac1 is a critical event downstream of the PI3K-Akt kinase cascade in insulin regulation of glucose transport in muscle cells. PMID: 23499910
    25. constitutive activity of Rac1, which is necessary for stretch-induced activation of the Rac1 downstream proteins, mediates stretch-induced increases in permeability and PJAR formation. PMID: 23686855
    26. The Atypical guanine nucleotide exchange factor Dock4 regulates neurite differentiation through modulation of Rac1 GTPase and actin dynamics. PMID: 23720743
    27. Geranylgeranyltransferase I mediates BDNF-induced neuronal synaptogenesis through Rac1 activation. PMID: 23534605
    28. These results indicate that activation of Rac1, which leads to reactive oxygen species generation, is required for neurite outgrowth induced by staurosporine in PC12 cells. PMID: 23153365
    29. Rac1 signalling contributes to doxorubicin-induced cardiotoxicity through both a ROS-dependent mechanism and ROS-independent HDAC/p53 signalling in cardiomyocytes. PMID: 23027656
    30. Rac1 signaling plays a key role in oxalate-induced renal injury. PMID: 21814770
    31. Rac1 activation plays a pivotal role in the migration and proliferation of aortic smooth muscle cells stimulated by PDGF-BB. PMID: 22322064
    32. Blockade of small GTPase Rac1 specifically prevents insulin-induced increases in dendritic spine density of hippocampal neurons. PMID: 21683721
    33. Active Rac1 potentiates mineralocorticoid receptor transactivation. PMID: 22232135
    34. It was concluded that NADPH oxidase activity and Rac1 GTP activity are indispensable for the nongenomic actions of mineralocorticoid receptors and that Rac1 GTP activation induces ERK1/2 phosphorylation in the brain. PMID: 22114025
    35. Peripheral nerve injury induces Rac1-regulated remodeling of dendritic spines on dorsal horn neurons, and suggests that this spine remodeling contributes to neuropathic pain. PMID: 21963650
    36. Rac1 GTPase in rodent kidneys is essential for salt-sensitive hypertension via a mineralocorticoid receptor-dependent pathway PMID: 21765214
    37. investigation of role of Rac1 in regulating vascular reactivity: In hemorrhagic shock models, data suggest that Rac1 has inhibitory effect on vascular reactivity mediated through activation of PAK, inhibition of MLCK, and inhibition of Rho kinase. PMID: 21386710
    38. phosphorylated Tiam2 activates Rac1 in neurons during neurite outgrowth PMID: 21460187
    39. Changes in Rac1 activity are involved in neurodegenerative processes following cerebral ischemia, and during its long-term recovery. PMID: 20817060
    40. in microvascular endothelium, Rac1-mediated alterations in contact zone architecture contribute to cAMP-induced barrier stabilization. PMID: 21457935
    41. Rac1 to inhibit intracellular superoxide production as well as overcome Bcl-2-mediated drug resistance in human leukemia cells and cervical cancer cells. PMID: 21474673
    42. data suggest that isoprenylcysteine carboxyl methyltransferase regulates glucose-induced Rac1 activation, generation of reactive oxygen species and insulin secretion in pancreatic beta-cells PMID: 21346419
    43. our findings suggest that Rac1 is essential for epithelial Na(+) channel activity and regulates the channel via WAVE proteins. PMID: 21464391
    44. breakpoint cluster region and active BCR-related gene have novel roles in the regulation of synaptic Rac1 signaling, synaptic plasticity, and learning and memory, and that excessive Rac1 activity negatively affects synaptic and cognitive functions PMID: 20962234
    45. Insulin stabilizes endothelial barrier via inactivation of the endothelial contractile machinery and enhancement of cell-cell adhesions. These effects are mediated via PI3K/Akt- and NO/cGMP-induced Rac1 activation. PMID: 20339116
    46. puerarin may exert inhibitory effects on HG-induced VSMC proliferation via interfering with PKCbeta2/Rac1-dependent ROS pathways, thus resulting in the attenuation of neointimal formation in the context of hyperglycemia in diabetes mellitus PMID: 19854265
    47. Physiological increases in NaCl stimulate thick ascending limb superoxide generation by activating Rac1. PMID: 19923407
    48. Effects of cell tension on the small GTPase Rac. PMID: 12105187
    49. essential for lipopolysaccharide signaling to extracellular signal-regulated kinase and p38 MAP kinase activation in rat-2 fibroblasts PMID: 12132588
    50. Rac1 induces cardiac myocyte hypertrophy mediated through ASK1 and NF-kappaB PMID: 12672819

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Cell projection, lamellipodium. Cell projection, dendrite. Cell junction, synapse.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 组织特异性：
    Osteoclasts.
  • 数据库链接：

    KEGG: rno:363875

    STRING: 10116.ENSRNOP00000001417

    UniGene: Rn.228585