Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Pyruvate kinase isozymes R/L (Pklr)

Recombinant Rat Pyruvate kinase isozymes R/L (Pklr)

  • 货号：
    CSB-EP018071RA
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    PklrPyruvate kinase PKLR; EC 2.7.1.40; L-PK; Pyruvate kinase isozymes L/R
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    66.2kDa
  • 表达区域：
    1-574aa
  • 氨基酸序列
    MSVQENTLPQQLWPWIFRSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a key role in glycolysis.
  • 基因功能参考文献：
    1. Studied the effect of the point mutations around this phosphorylation site on the catalytic properties of L-pyruvate kinase. PMID: 22878931
    2. c-Myc is required for the glucose-mediated induction of this metabolic enzyme. PMID: 12480946
    3. We observed that cystine inhibited the enzyme pyruvate kinase activity non-competitively in a dose- and time-dependent way. PMID: 15196592
    4. cooperative interaction between NF1 family members and HNF1alpha plays an important role in hepatic L-PK transcription PMID: 16204235
    5. Regulation of hepatic L-pyruvate kinase promoter composition and activity PMID: 16644726
    6. A novel chimeric promoter, consisting of a 1.6 kb glucose 6-phosphatase promoter and a segment of the regulatory element derived from the L-type pyruvate kinase promoter, was designed to provide strong and tight control of insulin expression in liver. PMID: 17160355
    7. Maximal glucose-induced expression of the L-PK gene in INS-1-derived 832/13 cells involves increased c-Myc abundance, recruitment of c-Myc, Max, and ChREBP to the promoter, and a glucose-stimulated increase in ChREBP transactivation. PMID: 17341548
    8. A detailed analysis of the molecular events of the insulin-independent glucose response of the liver-type pyruvate kinase (L-PK) gene was performed. PMID: 18468514
    9. cAMP and glucose signaling converge on a complex containing ChREBP, HNF4alpha, and CBP, and cAMP acts by disrupting this transcriptional complex assembled by glucose-derived signals PMID: 19406844
    10. Data show that glucose regulates acetylation and methylation of various histone residues at the L-PK gene promoter. PMID: 19631660

    显示更多

    收起更多

  • 蛋白家族：
    Pyruvate kinase family
  • 数据库链接：

    KEGG: rno:24651

    STRING: 10116.ENSRNOP00000027700

    UniGene: Rn.48821