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  4. Recombinant Rat Pyruvate kinase PKM (Pkm)

Recombinant Rat Pyruvate kinase PKM (Pkm)

In Stock
  • 货号：
    CSB-EP018072RA
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018072RA could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Pkm.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018072RA could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Pkm.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pkm; Pkm2; PykmPyruvate kinase PKM; EC 2.7.1.40; Pyruvate kinase muscle isozyme
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    62.8 kDa
  • 表达区域：
    1-531aa
  • 氨基酸序列
    MPKPDSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRAATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADYLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAVFHRLLFEELARASSQSTDPLEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRVNLAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. In addition to its role in glycolysis, also regulates transcription. Stimulates POU5F1-mediated transcriptional activation. Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages. Also acts as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs. Plays a general role in caspase independent cell death of tumor cells.
  • 基因功能参考文献：
    1. Monocrotaline-induced rats developed severe pulmonary arterial hypertension and right ventricular hypertrophy, with a significant increase in the phosphorylated PKM2 activity. PMID: 26774701
    2. After light-induced retinal damage in retinal ganglion cells PKM2 was up-regulated. PMID: 25990228
    3. Nuclear translocation of PKM2 promotes astrocytes proliferation after spinal cord injury through modulating p27 cell cycle signaling. PMID: 26151495
    4. PKM2 may regulate the survival of cardiomyocytes in acute rejection after heart transplantation in rat PMID: 25457184
    5. Data suggest M2PK is modulated in mast cell degranulation via IgE/FCERI (IgE high affinity I receptor) signaling; immediate inhibition of M2PK involves tyrosine phosphorylation; subsequently fructose-1,6-biphosphate accumulates and activates M2PK. PMID: 24497038
    6. PKM2-Oct4 interaction controls glioma cell death and differentiation. PMID: 24481450
    7. Data indicate that the beta-alanine administration was able to inhibit the enzyme pyruvate kinase, cytosolic creatine kinase, and adenylate kinase activities in cerebral cortex, and increase in cerebellum. PMID: 23620342
    8. PanK4 interacts with Pkm2 and thereby may modulate the glucose metabolism through regulating the activity of Pkm2. PMID: 16132722
    9. Age-dependent alterations in protein abundance indicated dramatic changes in metabolism, contractile activity, myofibrillar remodelling and stress response and decreased levels of pyruvate kinase. PMID: 18050275
    10. Pyruvate kinase inhibition caused by cystine released from lysosomes could be one of the mechanisms of tissue damage in patients with cystinosis. PMID: 18418703
    11. Regulation of M2-type pyruvate kinase mediated by the high-affinity IgE receptors is required for mast cell degranulation. PMID: 18587448

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Pyruvate kinase family
  • 数据库链接：

    KEGG: rno:25630

    STRING: 10116.ENSRNOP00000015331

    UniGene: Rn.1556