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  4. Recombinant Rat Protein kinase C zeta type (Prkcz)

Recombinant Rat Protein kinase C zeta type (Prkcz)

  • 货号：
    CSB-YP018710RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018710RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018710RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018710RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018710RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prkcz; PkczProtein kinase C zeta type; EC 2.7.11.13; nPKC-zeta
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-592
  • 氨基酸序列
    MPSRTDPKMD RSGGRVRLKA HYGGDILITS VDPTTTFQDL CEEVRDMCGL HQQHPLTLKW VDSEGDPCTV SSQMELEEAF RLACQGRDEV LIIHVFPSIP EQPGMPCPGE DKSIYRRGAR RWRKLYRANG HLFQAKRFNR RAYCGQCSER IWGLARQGYR CINCKLLVHK RCHVLVPLTC RRHMDSVMPS QEPPVDDKND GVDLPSEETD GIAYISSSRK HDNIKDDSED LKPVIDGVDG IKISQGLGLQ DFDLIRVIGR GSYAKVLLVR LKKNDQIYAM KVVKKELVHD DEDIDWVQTE KHVFEQASSN PFLVGLHSCF QTTSRLFLVI EYVNGGDLMF HMQRQRKLPE EHARFYAAEI CIALNFLHER GIIYRDLKLD NVLLDADGHI KLTDYGMCKE GLGPGDTTST FCGTPNYIAP EILRGEEYGF SVDWWALGVL MFEMMAGRSP FDIITDNPDM NTEDYLFQVI LEKPIRIPRF LSVKASHVLK GFLNKDPKER LGCRPQTGFS DIKSHAFFRS IDWDLLEKKQ TLPPFQPQIT DDYGLDNFDT QFTSEPVQLT PDDEDVIKRI DQSEFEGFEY INPLLLSAEE SV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro.; Involved in late synaptic long term potentiation phase in CA1 hippocampal cells and long term memory maintenance.
  • 基因功能参考文献：
    1. The current studies demonstrate that enhanced action potential firing produced by NGF was blocked by inhibitors of translation, but not transcription. In vitro studies showed that NGF elevated the protein levels of PKMzeta, which was also prevented by inhibitors of translation. Intraplantar injection of NGF produced a rapid and maintained increase in mechanical sensitivity whose onset was delayed by translation inhibit... PMID: 29288797
    2. the overexpression of PKMzeta in hippocampal neurons immobilized GluA2-containing AMPA receptors. PMID: 29202853
    3. Here we find that spatial conditioning by aversive active place avoidance or appetitive radial arm maze induces PKMzeta increases in dorsal hippocampus that persist from 1day to 1month, coinciding with the strength and duration of memory retention. PMID: 27417578
    4. In contrast to adult rats, juveniles failed to show contextual fear responses at 4 d post-fear conditioning. Reconsolidation sessions 3 and 6 d after conditioning restored contextual fear responses in juveniles 7 d after initial training. In juveniles that received reconsolidation sessions, protein kinase M zeta (PKMzeta) increased in the amygdala, but not in the hippocampus. PMID: 27918276
    5. PKMzeta, but not PKCiota, knock-down disrupted previously established long-time memory. PKCiota and PKMz have distinct functions in long-term potentiation and memory, with PKMzeta playing a specific role in memory maintenance. PMID: 27498875
    6. analysis of MAP2c phosphorylation and regulatory protein 14-3-3zeta-binding sites, which reveals key differences between MAP2c and its homolog Tau PMID: 28258221
    7. PKMzeta may not affect the neural circuits underlying spontaneous tasks that are different from aversive tasks PMID: 27076427
    8. The results of this study found that PKMzeta overexpression in the PrL but not IL enhanced the formation of fear memory, and PKMzeta in the PrL may contribute to the formation of long-term fear memory PMID: 25722116
    9. PKCzeta expression is elevated in hepatocytes of insulin resistant ZF rats. Overexpression in primary hepatocytes impairs insulin signal transduction, and in turn, the down-stream insulin-regulated gene expression. PMID: 25822413
    10. PKC zeta interacts with LEI/L-DNase II and controls its DNase activity by impairing its nuclear translocation. PMID: 25781645
    11. IL-6 activates PI3K and PKCzeta signaling and determines cardiac differentiation in rat embryonic H9c2 cells PMID: 26205888
    12. downstream effect of dietary PC is to activate PKCzeta PMID: 25713101
    13. Physical exercise reduced the REDD1 protein, increased 14-3-3 protein levels and P38MAPK phosphorylation. PMID: 24691733
    14. structural and molecular alterations of the outer blood retinal barrier during the time course of diabetes, focusing on PKCzeta PMID: 24312324
    15. Results provide the first evidence that KIBRA as well as PKMzeta is closely related to reference memory but not working memory in rats PMID: 24141945
    16. In hippocampal and cortical pyramidal cells, PKMzeta expression is predominantly somatodendritic, and electron microscopy highlights the kinase at postsynaptic densities and in clusters within spines. PMID: 24298142
    17. KIBRA levels in the rodent hippocampus correlate closely both to spatial memory performance in rodents and to PKMzeta PMID: 24117625
    18. These results suggest that PKMzeta is involved in regulation of dendritic spine structure and function, which may underlie its role in long-term synaptic and behavioral plasticity PMID: 22123937
    19. PKMzeta-dependent amplification contributes to nerve injury-induced aversiveness within the rostral anterior cingulate cortex. PMID: 22482911
    20. Data demonstrated that 14-3-3tau enhances the transcriptional activity of PR-B. PMID: 22967481
    21. Acute intrahippocampal PKMzeta inhibition disrupts place-cell activity in a familiar environment, where the map should be stable. Synaptic plasticity maintained by PKMzeta, which stabilizes the map, is needed for the proper spatial memory expression. PMID: 23035087
    22. These data contribute to a growing body of literature that demonstrates that PKMzeta plays a key role in maintaining amygdala-dependent memory. PMID: 22659643
    23. EMAP-II induces blood tumor barrier opening via the RhoA/Rho kinase/PKC-alpha/beta signaling pathways, however, PKC-zeta is involved in this process by other mechanisms. PMID: 22531886
    24. It was concluded that sevoflurane induces activation of the mTOR pathway, increasing the new protein synthesis of PKMzeta, which is constitutively phosphorylated to its active form, leading to an increased KATP channel-induced hyperpolarizaton. PMID: 22674720
    25. PKMzeta in the basolateral amygdala is required for maintenance of associative morphine reward memory; in the infralimbic cortex PKMzeta is required for the maintenance of extinction memory reward-related cues and morphine withdrawal-related aversive cues. PMID: 21633338
    26. results suggest that persistent activity of PKMzeta is a requisite for cocaine-induced enhancement of synaptic plasticity in the ventral tegmental area and cocaine conditioned place preference. PMID: 22153887
    27. results suggest that PKCzeta, especially PKMzeta isoform, is a significant factor involved in spinal persistent nociceptive processing, specifically, the manifestation of chronic pain states following peripheral inflammation. PMID: 22054645
    28. These results suggest spinal PKMzeta is essential for the maintenance of persistent pain by sustaining spinal nociceptive plasticity. PMID: 22185613
    29. inhibition of the atypical protein kinase C isoform PKCzeta and its constitutively active isoform PKMzeta with inhibitor ZIP administered locally into the nucleus accumbens core reversibly inhibited the retrieval of drug-associated memory PMID: 22348011
    30. This review discusses PKMzeta as necessary and sufficient for the maintenance of long-term potentiation and neuroplasticity in rat sensorimotor cortices. PMID: 21949908
    31. PKMzeta is unlikely to be the mediator of synaptic plasticity or memory. PMID: 22378786
    32. These results demonstrate that NGF leads to the activation of PKMzeta that ultimately enhances the capacity of small-diameter capsaicin-sensitive sensory neurons to fire APs through a PI3K-dependent signaling cascade. PMID: 21975456
    33. Dvl-aPKC interaction can promote axon differentiation mediated by the PAR3-PAR6-aPKC complex. PMID: 17558396
    34. Superoxide, PKC-zeta, and PP2A are involved in the hypoxia-induced increase in electrical conductance and occludin reduction at the plasma membrane in alveolar epithelial cells. PMID: 21257729
    35. These results suggest that PKMzeta inhibition does not erase memory, but temporarily disrupts expression of memory. PMID: 21258326
    36. Metaplasticity governs compartmentalization of synaptic tagging and capture through brain-derived neurotrophic factor (BDNF) and protein kinase Mzeta (PKMzeta). PMID: 21248226
    37. study reports that overexpression in the neocortex of the protein kinase C isozyme protein kinase Mzeta (PKMzeta) enhances long-term memory, whereas a dominant negative PKMzeta disrupts memory, even long after memory has been formed PMID: 21385716
    38. Src kinase plays an important role in AngII-elicited PKCzeta activation and the subsequent downstream signaling including ERK1/2 activation and VSMCs proliferation. PMID: 20307614
    39. the dorsal hippocampus mediates long-term memory for where, but not what things have been encountered, and that PKMzeta maintains this type of spatial knowledge as long as the memory exists. PMID: 19806657
    40. This study indicated that PKMzeta maintains long-term memory by regulating the trafficking of GluR2-containing AMPA receptors, the postsynaptic expression of which directly predicts memory retention. PMID: 20383136
    41. Translocation of PKCzeta may play a central role in the development of type 2 diabetes. PMID: 20013954
    42. Results show that although hypothalamic aPKC signaling is not required for food intake inhibition by insulin or leptin, it plays a key role in inflammatory anorexia and fever induced by LPS. PMID: 19819945
    43. Data show that the migration associated control of JNK by aPKCs determines JNK phosphorylation of the plasma membrane substrate paxillin. PMID: 19885391
    44. insulin-induced glucose uptake is mainly mediated by PI 3-kinase-PKCzeta signaling, whereas phorbol ester-induced glucose uptake is mainly mediated by conventional PKC despite PI 3-kinase and PKCzeta activations PMID: 11699875
    45. Alteration of protein kinase C isoforms in the liver of septic rat. PMID: 11795668
    46. 1alpha,25(OH)2D3 regulates chondrocyte matrix vesicle protein kinase C (PKC) directly via G-protein-dependent mechanisms and indirectly via incorporation of PKC during matrix vesicle biogenesis PMID: 11805100
    47. Protein kinase C-zeta phosphorylates insulin-responsive aminopeptidase in vitro at Ser-80 and Ser-91. PMID: 12061804
    48. Fatty acid infusion impairs insulin stimulated PKC activation PMID: 12095990
    49. role in regulating transcription of the matrix metalloproteinase-9 gene induced by IL-1 and TNF-alpha in glioma cells via NF-kappa B PMID: 12130632
    50. PLCgamma participates in insulin stimulation of glucose uptake through activation of PKCzeta in brown adipocytes. PMID: 12169270

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  • 亚细胞定位：
    Cytoplasm. Endosome. Cell junction. Membrane; Peripheral membrane protein.; [Isoform 2]: Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
  • 组织特异性：
    Isoform 1: In brain, expressed in hippocampus, neocortex and cerebellum (at protein level). Also expressed in lung, liver, kidney, testis and to a lesser extent in pancreas, intestine and skin (at protein level). Isoform 2: Specifically expressed in brain
  • 数据库链接：

    KEGG: rno:25522

    STRING: 10116.ENSRNOP00000021285

    UniGene: Rn.1109