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  4. Recombinant Rat Protein kinase C beta type (Prkcb)

Recombinant Rat Protein kinase C beta type (Prkcb)

  • 货号：
    CSB-YP018700RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018700RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018700RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018700RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018700RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prkcb; Pkcb; Prkcb1Protein kinase C beta type; PKC-B; PKC-beta; EC 2.7.11.13
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-671
  • 氨基酸序列
    ADPAAGPPP SEGEESTVRF ARKGALRQKN VHEVKNHKFT ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPASDDPRS KHKFKIHTYS SPTFCDHCGS LLYGLIHQGM KCDTCMMNVH KRCVMNVPSL CGTDHTERRG RIYIQAHIDR EVLIVVVRDA KNLVPMDPNG LSDPYVKLKL IPDPKSESKQ KTKTIKCSLN PEWNETFRFQ LKESDKDRRL SVEIWDWDLT SRNDFMGSLS FGISELQKAG VDGWFKLLSQ EEGEYFNVPV PPEGSEGNEE LRQKFERAKI GQGTKAPEEK TANTISKFDN NGNRDRMKLT DFNFLMVLGK GSFGKVMLSE RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKEHAFF RYIDWEKLER KEIQPPYKPK ARDKRDTSNF DKEFTRQPVE LTPTDKLFIM NLDQNEFAGF SYTNPEFVIN V
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
  • 基因功能参考文献：
    1. data suggest that TRB3 mediates AGE-induced oxidative injury in beta-cells through the PKCbeta2 pathway. PMID: 28534945
    2. The s took a proteomics approach using a PKCB knockout mast cell model and found that the key mast cell secretory protein VAMP8 becomes phosphorylated by PKC at multiple residues in the SNARE domain. PMID: 27402227
    3. reduced GLP-1R expression in spontaneously hypertensive rat renal arteries most likely mediated through PKCbeta upregulation PMID: 25915883
    4. PRKCBII and PRKCD-dependent inactivation of p38 regulates cardiac fibroblast proliferation and collagen deposition in response to angiotensin II. PMID: 25659900
    5. PKCbeta2 inhibition protects diabetic hearts from myocardial ischemia/reperfusion injury through Cav-3-dependent activation of Akt. PMID: 25849791
    6. Urotensin-II potentiates ICa,L through the beta-gamma subunits of Gi/o-protein and downstream activation of the class I PI3K-dependent PKCB1 isoform. PMID: 25678708
    7. these data indicate a role of PKCbetaII in advanced glycation endproduct accumulation-induced myopathic changes in rat cardiomyocytes PMID: 24269213
    8. In the kidneys of bile duct ligated rats melatonin inhibits protein kinase C beta to reduce p47phox superoxide production. PMID: 23295407
    9. Exercise-induced benefits on diabetic myocardium are mediated by the modulation of molecules involved in PKC-betaII signaling. PMID: 22797313
    10. In the diabetic heart, the RhoA/ROCK pathway contributes to contractile dysfunction at least in part by sustaining PKCbeta(2) activation and reactive oxygen species production via a positive feedback loop that requires an intact cytoskeleton. PMID: 22865386
    11. Results provide evidence PKCbeta(II) modulates contractile function via intermediate downstream pathway(s) in cardiac myocytes. PMID: 22587992
    12. Data indicate PKCbetaII as a novel inhibitor of proteasomal function. PMID: 22479367
    13. PKCbeta acting through mitochondrial proteins could play a role in protecting the cells from death by e.g. influencing ROS and ATP production after ischemia in CA2-4,DG region of the hippocampus. PMID: 21704734
    14. Report amelioration of the pathological findings of experimental mesangial proliferative glomerulonephritis by PKC-beta inhibition. PMID: 21575103
    15. Data indicate that the selective inhibition of PKCbetaII attenuates cardiac remodelling mediated by the TGF-SMAD signalling pathway. PMID: 20874717
    16. PRKCB2 (protein kinase C beta2) and PKCdelta (protein kinase C delta) counter-regulate coronary endothelial barrier properties by targeting distinctive subcellular sites. PMID: 18497307
    17. Wxpression of PKC-betaII in renal cortex of diabetic rats decreased significantly in membrane and remained unchanged in cytosol compared with normal controls. PMID: 16837815
    18. Glomerular-specific protein kinase C-beta-induced insulin receptor substrate-1 dysfunction and insulin resistance in models of diabetes and obesity. PMID: 21228767
    19. Protein kinase C upregulates intercellular adhesion molecule-1 and leukocyte-endothelium interactions in hyperglycemia via activation of endothelial expressed calpain. PMID: 21071702
    20. these results show how PKCbetaII is allosterically regulated in two steps, with the second step defining a novel protein kinase regulatory mechanism. PMID: 21215369
    21. These findings demonstrate the activation, in the retina, of the PKCbeta/HuR/VEGF pathway following experimental diabetes and disclose a new potential pharmacological target. PMID: 20599775
    22. Adenosine triphosphate (ATP) regulates volume-regulated anion channel activity and volume-sensitive excitatory amino acid release via cooperative activation of PKCalpha and beta I in primary cortical astrocytes. PMID: 18315563
    23. puerarin may exert inhibitory effects on HG-induced VSMC proliferation via interfering with PKCbeta2/Rac1-dependent ROS pathways, thus resulting in the attenuation of neointimal formation in the context of hyperglycemia in diabetes mellitus PMID: 19854265
    24. PKCbetaII and PKCdelta counter-regulate BBB permeability during aglycemic hypoxia. PMID: 19900507
    25. Data indicate that the high glucose+LY333531 group showed noticeable attenuation on both permeability and apoptosis with reduced PKCbeta II expression. PMID: 19351495
    26. PKCbeta-MARCKS signaling may be a general mechanism for the stimulation of vesicular trafficking in brain neurons. PMID: 11882609
    27. Phosphoinositide 3-kinase mediates protein kinase C beta II mRNA destabilization in rat A10 smooth muscle cell cultures exposed to high glucose PMID: 12061808
    28. findings demonstrate a requirement for the PKC-betaI and -delta signal transduction pathways in hexosamine biosynthesis pathway-induced transcription PMID: 12105191
    29. Translocation of PKC isotypes with changes in GAP-43 phosphorylation suggested that PKCbetaII and PKCepsilon may mediate early changes in gap-43 phosphorylation and increases in GAP-43 expression after contextual fear conditioning. PMID: 12201630
    30. Inhibition of this enzyme attenuates the progression of experimental diabetic nephropathy in the presence of continued hypertension. PMID: 12540629
    31. PKCbeta may play an important role in glucocorticoid-induced insulin resistance PMID: 12665248
    32. protein kinase C beta II may be one of the diacylglycerol sensitive isoforms involved in glucose transport [protein kinase C beta II] PMID: 12726887
    33. GLUT2-mediated fructose absorption is regulated through phosphatidylinositol 3-kinase and mammalian target of rapamycin-dependent pathways, which control phosphorylation of protein kinase C betaII PMID: 12766174
    34. The overexpression of both PKC-betaI and -delta in old rats could cause the hyperphosphorylation of Na-K-ATPase and contributes to the diminished inhibition of Na-K-ATPase activity by dopamine in old rats. PMID: 12904329
    35. PKC betaII colocalizes with pericentrin at centrosomes. Disruption of this interaction by expression of the interacting region of pericentrin results in release of PKC from the centrosome, microtubule disorganization, and cytokinesis failure. PMID: 14594954
    36. 1alpha,25-Dihydroxy vitamin D3 reduces insulin-induced glucose uptake via activation of PKC(beta) in rat adipocytes PMID: 14682474
    37. Activation of PKC-betaII in endothelial cells during obesity suppressed NO regulation both at rest and in response to increased flow velocity and decreased oxygen availability. PMID: 14715497
    38. PKCbetaII induces cell invasion through a Ras/Mek-, PKC iota/Rac 1-dependent signaling pathway PMID: 15037605
    39. in astrocytes, RACK1 and PKC-betaII synchronous relocation is not essential for relocation of PKC-betaII to the plasma membrane PMID: 15042584
    40. Protein kinase CbetaII regulates its own expression in rat intestinal epithelial cells and the colonic epithelium in vivo. PMID: 15322124
    41. classical PKC beta(II) is physically associated with DAT and is important in maintaining the amphetamine-stimulated outward transport of dopamine in rat striatum. PMID: 15647254
    42. These results indicate that RasGRP3 is implicated in phorbol ester-induced, PKC-independent exocytosis. PMID: 15737652
    43. This evidence supports that the Hal gene is turned on by glucocorticoids and by glucagon either via PKC or PKA, but prefers the PKA pathway. PMID: 15741241
    44. chronological expression of PKC isoforms in dental follicle follows different profiles; expression of PKCb was lowest at day 1 then elevated from day 3 to day 11; study suggests that PKC isoforms may be involved in the regulation of tooth eruption PMID: 15763930
    45. the biological effects of PKCdelta include positive regulation of G(1) phase cell cycle progression PMID: 16051606
    46. We conclude that in HG, mesangial cell ROS generation by upregulated NADPH oxidase is dependent on conventional PKC isozymes (PKC-b) and also required for collagen IV expression. PMID: 16131649
    47. Data indicate that protein kinase C betaI activation represents a key factor for the protection of the integrity of neural elements in the cochlea. PMID: 16179609
    48. TCDD activates PKC-betaII via receptor for activated C kinase-1 in an aryl hydrocarbon receptor-dependent manner. PMID: 16797713
    49. An increase in protein kinase C (PKC) beta1 and beta2 isoform content neither plays an important role during the development of cardiac hypertrophy nor participates in the phase leading to heart failure owing to volume overload. PMID: 16900949
    50. We conclude that fear conditioning causes hemisphere and input specific increases in PKC betaII in the rat basolateral amygdala. PMID: 17118565

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
  • 数据库链接：

    KEGG: rno:25023

    STRING: 10116.ENSRNOP00000016417

    UniGene: Rn.91118