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  4. Recombinant Rat Protein arginine N-methyltransferase 1 (Prmt1)

Recombinant Rat Protein arginine N-methyltransferase 1 (Prmt1)

  • 货号：
    CSB-YP723546RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP723546RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP723546RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP723546RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP723546RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prmt1; Hrmt1l2Protein arginine N-methyltransferase 1; EC 2.1.1.319; Histone-arginine N-methyltransferase PRMT1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-353
  • 氨基酸序列
    MAAAEAANCI MEVSCGQAES SEKPNAEDMT SKDYYFDSYA HFGIHEEMLK DEVRTLTYRN SMFHNRHLFK DKVVLDVGSG TGILCMFAAK AGARKVIGIE CSSISDYAVK IVKANKLDHV VTIIKGKVEE VELPVEKVDI IISEWMGYCL FYESMLNTVL HARDKWLAPD GLIFPDRATL YVTAIEDRQY KDYKIHWWEN VYGFDMSCIK DVAIKEPLVD VVDPKQLVTN ACLIKEVDIY TVKVEDLTFT SPFCLQVKRN DYVHALVAYF NIEFTRCHKR TGFSTSPESP YTHWKQTVFY MEDYLTVKTG EEIFGTIGMR PNAKNNRDLD FTIDLDFKGQ LCELSCSTDY RMR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity. Plays a role in regulating alternative splicing in the heart.
  • 基因功能参考文献：
    1. PRMT1 might have a potential importance as a therapeutic target for the treatment of Hypobaric hypoxia-induced maladies. PMID: 29128891
    2. Higher levels of asymmetric dimethylarginine are associated with lower DDAH2 and higher PRMT1 in LPS-induced endometritis rat model. PMID: 29119338
    3. The detailed mechanism of PRMT1-catalyzed asymmetric dimethylation, is reported. PMID: 23977297
    4. higher energy barrier of SDMA formation (DeltaDeltaG(double dagger) = 3.2 kcal/mol as compared with ADMA) may explain the small amount of SDMA generated by M48F-PRMT1 PMID: 24478314
    5. Study reports that PRMT1 methylates a multisite peptide substrate in a nonstochastic manner, with less C-terminal preference, consistent with the methylation patterns observed in vivo. With a single targeted arginine, PRMT1 catalyzed the dimethylation in a semiprocessive manner. PMID: 23214442
    6. PRMT1 and DDAH-1, responsible for the biosynthesis and degradation of asymmetric dimethylarginine respectively, are expressed in erythrocytes, leukocytes, and platelets. PMID: 22785485
    7. substrate preference of protein arginine methyltransferase I PMID: 22697391
    8. PRMT1 was expressed in bronchus & alveolus epithelium & upregulated in lungs from AIPI rats. The inhibition of PRMT downregulated eotaxin-1 & CCR3 induction by IL-4 in epithelial cells. PRMT1 plays an important role in asthma pathogenesis. PMID: 22387551
    9. data give direct evidence that the chemistry of methyl transfer is the major rate-limiting step and that binding of the cofactor SAM or SAH affects the association and dissociation of H4 with PRMT1 PMID: 21736313
    10. Showed ectopically expressed PRMT1 in mammalian HEK293 cells not only exhibited catalytic properties comparable to endogenous enzyme but also existed in a functional complex together with endogenous PRMT1 and thus functioned as an endogenous counterpart. PMID: 21080372
    11. These data suggest that the PRMT1-mediated methylation of hnRNPQ is implicated in insulin receptor trafficking and insulin signaling in skeletal L6 myotubes. PMID: 18492485
    12. This study aims to investigate whether alteration of PRMT1 activity contributes to the clinical features of type 2 diabetes. PMID: 19467247

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytosol. Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: rno:60421

    STRING: 10116.ENSRNOP00000063624

    UniGene: Rn.5870