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  4. Recombinant Rat Protein Dom3Z (Dom3z)

Recombinant Rat Protein Dom3Z (Dom3z)

  • 货号：
    CSB-YP740499RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP740499RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP740499RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP740499RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP740499RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dxo
  • Uniprot No.：
  • 别名：
    Dxo; Dom3zDecapping and exoribonuclease protein; DXO; EC 3.1.13.-; EC 3.6.1.-; Dom-3 homolog Z
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-397
  • 氨基酸序列
    MEPRGTKRKA EKTEVAKTWN KLPRSLPSLR TQPSLYSGPF PFYRRPSELG CFSLDAQRQY HGDARALRYY SPPPINGPGP DFDLRDGYPD RYQPRDEEVR ERLDHLLRWV LEHRNQLEGG PGWLAGATVT WRGHLTKLLT TPYERQEGWQ LAASRFQGTL YLSEVETPAA RAQRLARPPL LRELMYMGYK FEQYMCADKP GGSPDPSGEV NTNVAFCSVL RSRLGNHPLL FSGEVDCLNP QAPCTQPPSC YVELKTSKEM HSPGQWRSFY RHKLLKWWAQ SFLPGVPYVV AGFRNPEGFV CSLKTFPTME MFENVRNDQE GWNPSVCMNF CAAFLSFAQS TVVQDDPRLV HLFSWEPGGP VTVSVHRDAP YAFLPSWYVE AMTQDLPSLS KTPSPKD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to environmental stress. Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation. Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control. Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1). In contrast to canonical decapping enzymes DCP2 and NUDT16, which cleave the cap within the triphosphate linkage, the decapping activity releases the entire cap structure GpppN and a 5'-end monophosphate RNA. Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates. Exhibits decapping activity towards FAD-capped RNAs. Exhibits decapping activity towards dpCoA-capped RNAs in vitro.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DXO/Dom3Z family
  • 数据库链接：

    KEGG: rno:361799

    STRING: 10116.ENSRNOP00000000483

    UniGene: Rn.20463