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  4. Recombinant Rat Pro-MCH (Pmch)

Recombinant Rat Pro-MCH (Pmch)

  • 中文名称：
    大鼠Pmch重组蛋白
  • 货号：
    CSB-YP018230RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Pmch重组蛋白
  • 货号：
    CSB-EP018230RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Pmch重组蛋白
  • 货号：
    CSB-EP018230RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Pmch重组蛋白
  • 货号：
    CSB-BP018230RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Pmch重组蛋白
  • 货号：
    CSB-MP018230RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pmch
  • Uniprot No.：
  • 别名：
    Pmch; MchPro-MCH [Cleaved into: Neuropeptide-glycine-glutamic acid; NGE; Neuropeptide G-E); Neuropeptide-glutamic acid-isoleucine; NEI; Neuropeptide E-I); Melanin-concentrating hormone; MCH)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-165
  • 氨基酸序列
    ILLSASKSI RNVEDDIVFN TFRMGKAFQK EDTAERSVVA PSLEGYKNDE SGFMKDDDDK TTKNTGSKQN LVTHGLPLSL AVKPYLALKG PAVFPAENGV QNTESTQEKR EIGDEENSAK FPIGRRDFDM LRCMLGRVYR PCWQV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    MCH inhibits ACTH secretion at the end of the light on period which corresponds to the peak of the circadian rhythm in ACTH. Inhibits also stress induced ACTH release during the light off period of the cycle. Involved as a neurotransmitter or neuromodulator in a broad array of neuronal functions. Stimulates sexual behavior when injected into the ventromedial nucleus, this effect is antagonized by NEI. In the medial preoptic area, stimulates anxiety and sexual behavior. Antagonizes inhibitory effect of melanotropin alpha on exploration behavior.; NEI can influence differentiation of neuronal processes in brain neurons. Affects the content of neurofilament protein in neuritogenesis (in vitro). May also be a neuromodulatory factor. In behavioral tests, it stimulates exploration and anxiety when injected into the ventromedial nucleus. Also stimulates grooming, locomotion and rearing. May antagonize the inhibitory effect of mch on ACTH release. Reduces dopamine and dopac release in the ventromedial nucleus.
  • 基因功能参考文献：
    1. results show that the number of Melanin Concentrating hormone-immunoreactive neurons in the Medial Preoptic Area is positively correlated with the number of pups in the litter and that artificially reducing the number of pups can also decrease the number of MCH-immunoreactive neurons in the MPOA. PMID: 28867197
    2. our findings demonstrate that NEI, a cleaved peptide from the same precursor, prepro-MCH, as MCH, induce REM sleep at least in part through acting on the vlPAG PMID: 27845162
    3. MCH levels in the CSF, brain preproMCH and MCHR1 gene expression during paradoxical sleep deprivation, sleep rebound and chronic sleep restriction were studied in rats. PMID: 26456505
    4. MCH participates not only in the regulation of glucose and sleep-wake homeostasis but also the vigilance-state dependent regulation of the central hypercapnic chemoreflex and respiratory control. PMID: 25084113
    5. modulation of mPOA function by MCH may contribute to the weaning of maternal responsiveness characteristic of the late postpartum period. PMID: 24893251
    6. Results indicate that melanin-concentrating hormone and orexin peptides play physiologically distinct roles in feeding behavior. PMID: 24780894
    7. Melanin-concentrating hormone neurons do not play a role in the short-term regulation of food intake in rats. PMID: 24676683
    8. MCH actually increases the hippocampal Aacetylcholine release at least in part through the medial septum in rats. PMID: 23531605
    9. Pmch-deficiency in rats is associated with normal adipocyte differentiation and lower sympathetic adipose drive. PMID: 23555928
    10. MCH generates sleep by regulating ventrolateral preoptic area neuronal activity in rats PMID: 23123302
    11. we suggested that MCH neurons in the locus coeruleus provide preferential projections to the brainstem cholinergic and aminergic nuclei PMID: 22967922
    12. Data indicate that Mch/Mchr1 signal transduction participates in modulation of depression-like behavior through processes in CA1 region of hippocampus. Model of depression involves chronically higher Mch expression and down-regulation of Mchr1. PMID: 22209364
    13. Data suggest that MCH and its receptor (MCHR1) form an orexigenic system whose expression is affected in conditioned taste aversion. MCH expression is up-regulated in hypothalamus (and possibly amygdala) of aversive animals. PMID: 21925200
    14. Melanin-concentrating hormone is expressed in the laterodorsal tegmental nucleus only in female rats. PMID: 17683785
    15. Hypo- and hyperthyroidism affect neuropeptide EI (NEI) concentration in discrete brain areas of adult male rats. PMID: 21530599
    16. NEI might induce LH secretion by direct release into the median eminence, by modulation of GnRH neurons located in the preoptic area, by modulation of GnRH terminals located in the median eminence or by an additive effect [nei peptide] PMID: 17016031
    17. Slices of rat hippocampus were perfused with different concentration of MCH: long-lasting potentiation on the hippocampal evoked response on dentate gyrus induced by MCH (4-11 microM) at 30, 60 and 120 min with a maximum effect at 120 min PMID: 11814630
    18. The rostromedial zona incerta expresses melanin-concentrating hormone, along with dopamine, in neurons, but they are not co-localized. PMID: 12706266
    19. role as an orexigenic neuropeptide and also suggest anti-anxiety properties PMID: 12832098
    20. Hypothalamic levels of melanin-concentrating hormone mRNA during pregnancy and lactation. PMID: 12890692
    21. MCH blockade led to lower body weight, decreased liver glycogen, increased body fat, increased interscapular brown adipose tissue mass, increased uncoupling protein 1 expression in brown adipose tissue, and increased consumption of lean body mass. PMID: 12960043
    22. Melanin-concentrating hormone [MCH] is a powerful hypnogenic factor. MCH neurons might play a key role in the state of paradoxical sleep via their widespread projections in the central nervous system PMID: 12964948
    23. glucocorticoids may promote food intake in part by potentiating the orexigenic actions of MCH without affecting the actions of orexin A and glucocorticoids act indirectly to influence the effects of MCH on food intake. PMID: 15044362
    24. MCH is involved in the regulation of LHRH release in the female rat. PMID: 15567347
    25. Existence of projection-dependent MCH neuronal subpopulations with functional differentiation in the context of controlling energy homeostasis, autonomic functioning, and arousal. PMID: 17151950
    26. NPW is involved in the regulation of feeding and/or sleep/arousal behavior through orexin- and/or MCH-mediated neuronal pathways PMID: 17884195
    27. presynaptic premotor neurons synthesizing either MCH or orexin are present within lateral hypothalamus, where they form two separate populations PMID: 17924541
    28. Results describe the distribution of RFRP neurons in the rat brain, and compare it to those of melanin-concentrating hormone neurons and hypocretin neurons. PMID: 19101033
    29. Melanin-concentrating hormone neurons discharge in a reciprocal manner to orexin neurons across the sleep-wake cycle PMID: 19188611

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Melanin-concentrating hormone family
  • 组织特异性：
    MCH is present in all regions of the brain and in neurointermediate lobe of the pituarity gland, with highest concentrations in the hypothalamus. Also expressed to a much lesser extent in stomach, lamina propria of both duodenum and colon, ovary, thymus,
  • 数据库链接：

    KEGG: rno:24659

    STRING: 10116.ENSRNOP00000006174

    UniGene: Rn.9675