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  4. Recombinant Rat Phosphatidylinositol-binding clathrin assembly protein (Picalm)

Recombinant Rat Phosphatidylinositol-binding clathrin assembly protein (Picalm)

  • 货号：
    CSB-YP017962RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017962RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017962RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017962RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017962RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Picalm
  • Uniprot No.：
  • 别名：
    Picalm; Calm; Phosphatidylinositol-binding clathrin assembly protein; Clathrin assembly lymphoid myeloid leukemia protein; rCALM
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-640
  • 氨基酸序列
    SGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN EMNVNIPQLA DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR NTLFNLSNFL DKSGLQGYDM STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR GADGVMRTMN TEKLLKTVPI IQNQMDALLD FNVNSNELTN GVINAAFMLL FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF LTRMTRISEF LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP HTSLTTAASP VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT FHPSVHAMSA APQVASTWGD AVDDAIPSLN PFLTKSSGDV HLPISSDVST FTTRTPTHEM FVGFSPSPVT QPHPSAGLNV DFESVFGNKS TNVAVDSGGG LLKPTVASQN QSLPVAKLPP NKLVSDDLDS SLANLVGNLG IGNGTTKNDV SCSQPGEKKL TGGSNWQPKV APTTAWSAAT MAPPVMAYPA TTPTGMIGYG IPPQMGSVPV MTQPTLIYSQ PVMRPPNPFG PVPGAQIQFM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).
  • 基因功能参考文献：
    1. Neurons with reduced reduced CALM had smaller Synaptophysin::EGFP-illuminated puncta. Synaptic vesicles were enlarged & their density and size were reduced. PMID: 22851330
    2. CALM is found nonselectively in pre- and postsynaptic profiles and also in perisynaptic processes. Observations reveal an unexpected relationship between AP180 and the presumed non-neuronal homologue CALM. PMID: 15558718
    3. High expression levels of CALM were found in hippocampal tissues as early as embryonic day 18; CALM was found in synapses at all developmental stages and in nonsynaptic growing processes. PMID: 17640037
  • 亚细胞定位：
    Cell membrane. Membrane, clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus.
  • 蛋白家族：
    PICALM/SNAP91 family
  • 组织特异性：
    Isoform 2 was found in most tissues examined. Isoform 1 has an overlapping expression pattern but is absent from lung, heart and pancreas. Both isoforms are widely expressed in the brain, higher levels are seen in hippocampus, dentate gyrus, medial habenu
  • 数据库链接：

    KEGG: rno:89816

    UniGene: Rn.24871