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  4. Recombinant Rat Nuclear receptor subfamily 4 group A member 1 (Nr4a1)

Recombinant Rat Nuclear receptor subfamily 4 group A member 1 (Nr4a1)

  • 货号：
    CSB-YP016062RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016062RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016062RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016062RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016062RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nr4a1; Hmr; Ngfib; Nuclear receptor subfamily 4 group A member 1; NUR77; Nerve growth factor-induced protein I-B; NGFI-B; Orphan nuclear receptor HMR
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-597
  • 氨基酸序列
    MPCIQAQYGT PATSPGPRDH LTGDPLALEF SKPTMDLASP ETAPTAPATL PSFSTFMDGG YTGEFDTFLY QLPGTAQPCS SASSTSSSSS SATSPASASF KFEDFQVYGC YPGTLSGPLD ETLSSSGSDY YGSPCSAPSP PTPNFQPSQL SPWDGSFGHF SPSQTYEGLR VWTEQLPKAS GPPPPPTFFS FSPPTGPSPS LAQSSLKLFP APATHQLGEG ESYSVPAAFP GLAPTSPNCD TSGILDAPVT STKARSGSSG GSEGRCAVCG DNASCQHYGV RTCEGCKGFF KRTVQKSAKY ICLANKDCPV DKRRRNRCQF CRFQKCLAVG MVKEVVRTDS LKGRRGRLPS KPKQPPDASP TNLLTSLIRA HLDSGPNTAK LDYSKFQELV LPRFGKEDAG DVQQFYDLLS GSLDVIRKWA EKIPGFIELS PGDQDLLLES AFLELFILRL AYRSKPGEGK LIFCSGLVLH RLQCARGFGD WIDNILAFSR SLHSLGVDVP AFACLSALVL ITDRHGLQDP RRVEELQNRI ASCLKEHMAA VAGDPQPASC LSRLLGKLPE LRTLCTQGLQ RIFCLKLEDL VPPPPIVDKI FMDTLSF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury.
  • 基因功能参考文献：
    1. Nur77 decreases ET-1 expression by suppressing NF-kappaB and p38 MAPK. PMID: 27765761
    2. IGFBP-3 may trigger osteoarthritis by inducing chondrocyte apoptotis through nucleus-mitochondria translocation of Nur77. PMID: 26884829
    3. Data indicate that orphan nuclear receptor Nur77 may be a sensor of oxidative stress and an inhibitor of vascular remodeling induced by low shear stress. PMID: 26498924
    4. Results suggest that Nur77-targeted siRNA can inhibit autologous vein graft stenosis. PMID: 26276525
    5. upregulation of Nur77 might be involved in Schwann cells differentiation and neurite elongation following sciatic nerve crush. PMID: 25957997
    6. Data suggest that a dietary component, the medium-chain fatty acid decanoate, can down-regulate expression of Hsd3b2 in adrenals/ovaries in model of polycystic ovary syndrome; inhibition of recruitment of Nur77 to Hsd3b2 promoter is involved. PMID: 26465200
    7. Results demonstrated that upregulated Nur77 may promote graft-infiltrating T lymphocyte apoptosis by translocating and inducing HtrA2/Omi release from mitochondria in acute rejection after cardiac transplantation. PMID: 26008781
    8. Nur77 functionally interacts with NFATc3 and GATA4 and inhibits their transcriptional activities. PMID: 26195821
    9. This study demonstrated the proapoptotic effect of Nur77 promotes cerebral cell apoptosis and induces early brain injury after experimental subarachnoid hemorrhage in rats. PMID: 24737679
    10. The loss of Nr4a1 in immune cells appears to cause the increased kidney injury and reduced renal function observed in the Nr4a1(-/-) model. PMID: 24722447
    11. Accompanied by the substantial decline in apoptosis inducer Nur77, myocardial ischemia/reperfusion induced cardiac dysfunction was manifested as cardiomyopathy and increased Reactive Oxygen Spieces. PMID: 24680679
    12. we found that survivin is highly inducible following oxygen and glucose deprivation and is required for NR4A1 action PMID: 23663895
    13. NR4A1 serves as an important point of negative feedback to minimize the excessive preantral follicle growth in hyperandrogenism. PMID: 23028064
    14. PDGF-induced vascular smooth muscle cell proliferation was related to the Nur77 expression and CREB phosphorylation. Atorvastatin reduced the Nur77 expression and, at the same time, inhibited VSMC proliferation. PMID: 21505978
    15. Stress affects cardiomyocytes by inducing NGFI-B mitochondrial translocation via serine phosphorylation, which in turn initiates mitochondrial-mediated apoptosis. PMID: 22128883
    16. Stress could induce NGFI-B translocation from the nucleus to the mitochondria in cardiomyocytes, which activated the mitochondrial pathway of cell apoptosis. PMID: 22097717
    17. Aetylation of Nrf2, p53 and Nur77 by CBP/p300 may constitute a novel similar regulatory mechanism for low-molecular-weight compounds with neurotrophic activities. PMID: 21908547
    18. This study results showed that acute and repeated stress modulates Nur77 expression in BNST and suggest that Nur77 participates in extra-hypothalamic responses to stress. PMID: 21442465
    19. Nur77 is a potential mechanism for improved skeletal muscle metabolism in response to exercise training. PMID: 21048074
    20. Nur77 promotes VSMC proliferation PMID: 20411306
    21. These results suggest that the expression of nur77, which is essential for the neuronal differentiation induced by dbcAMP (dibutyryl-cAMP ), is up-regulated via dbcAMP-induced acetylation of the Lys14 of histone H3 in PC12 cells. PMID: 20375114
    22. NGFI-B induces cardiomyocyte apoptosis in restraint-stressed rats by mediating mitochondrial energy metabolism disorder PMID: 19412742
    23. These findings suggest that NGFI-Bbeta plays an important role during the postmitotic differentiation of neuronal cells in the brain and retina. PMID: 19789410
    24. Cells exposed to dibutyltindichloride plus a calcium chelator reduce the transcription of nur77. This confirms the role of calcium in induction of nur77. PMID: 12030839
    25. The cell-type specific expression and LH induction of NGFI-B suggests a potential role of NGFI-B in the ovulatory process. PMID: 12770726
    26. Nur77, which is regulated by a MAPK pathway activated via arrestin 2, modulates NK(1)R-mediated nonapoptotic programmed cell death PMID: 14769794
    27. cadmium induces the expression of Nur77 family genes, leading to apoptosis in lung cells PMID: 15016657
    28. characterization of rat orphan nuclear receptor NOR-1 LBD domain PMID: 15358368
    29. Causal relationship between the rapid decline of aromatase mRNA and induction of orphan nuclear receptors NURR1 and NGFI-B expression, which concomitantly occur upon LH surge at the later stages of ovarian follicular development. PMID: 15486232
    30. Expression of the NGFI-B gene in the rat pineal gland was found to exhibit robust circadian rhythm. NGFI-B mRNA and protein levels are elevated via activation of adrenergic receptors and expression also involves the AP-1 protein Fra-2 PMID: 15525348
    31. In conclusion, our results suggest that cAMP-induced and Ca2+-induced processes may differentially regulate activity of Nur77 at the level of translocation of Nur77 protein from the cytoplasm into the nucleus. PMID: 15707695
    32. expression of NOR-1 is differentially regulated in different brain regions in response to globally applied brain ischemia, but that hypoxia is not sufficient to induce its expression PMID: 16951544
    33. Potential mediator of neuromuscular signaling in the control of metabolic gene expression. PMID: 17550977
    34. The rapid induction of NGFI-B/Nur77 gene expression by LH/hCG in MA-10 cells appears to be mediated by both CREB and Jun family proteins through the cAMP-protein kinase A (PKA) pathway. PMID: 18163434
    35. NUR77 and c-JUN have additive effects on the Star promoter. PMID: 18987158
    36. The transcription factor C/EBPbeta can activate the Nur77 promoter through a functional cooperation between the p50 subunit of NF-kappaB. PMID: 18996961
    37. Results suggest adrenergic stimulation as the most important stimulus for nur77 expression and point to a significant role for this transcription factor in adaptive changes in muscle after exercise and in insulin resistant states. PMID: 19447175

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Mitochondrion.
  • 蛋白家族：
    Nuclear hormone receptor family, NR4 subfamily
  • 组织特异性：
    Expressed in lung, brain and superior cervical ganglia. High levels are seen in the adrenal tissue.
  • 数据库链接：

    KEGG: rno:79240

    STRING: 10116.ENSRNOP00000010171

    UniGene: Rn.10000