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  4. Recombinant Rat Non-muscle caldesmon (Cald1)

Recombinant Rat Non-muscle caldesmon (Cald1)

  • 货号：
    CSB-YP720315RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP720315RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP720315RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP720315RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP720315RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cald1Non-muscle caldesmon; CDM; L-caldesmon
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-531
  • 氨基酸序列
    MLSRSGSQGR RCLATLSQIA YQRNDDDEEE AARERRRRAR QERLRQKQEE ESLGQVTDQV EAHVQNSAPD EESKPATANA QVEGDEEAAL LERLARREER RQKRLQEALE RQKEFDPTIT DGSLSVPSRR MQNNSAENET AEGEEKGESR SGRYEMEETE VVITSYQKNS YQDAEDKKKE EKEEEEEEEK LKGGNLGENQ IKDEKIKKDK EPKEEVKNFL DRKKGFTEVK AQNGEFMTHK LKQTENAFSP SRSGGRASGD KEAEGAPQVE AGKRLEELRR RRGETESEEF EKLKQKQQEA ALELEELKKK REERRKVLEE EEQRRKQEEA DRKAREEEEK RRLKEEIERR RAEAAEKRQK MPEDGLSEDK KPFKCFTPKG SSLKIEERAE FLNKSVQKSG VKSTHQAAVV SKIDSRLEQY TNAIEGTKAS KPMKPAASDL PVPAEGVRNI KSMWEKGSVF SSPSASGTPN KETAGLKVGV SSRINEWLTK SPDGNKSPAP KPSDLRPGDV SGKRNLWEKQ SVDKVTSPTK V
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration.
  • 基因功能参考文献：
    1. distribution of the actin-binding protein caldesmon in spleen and lymph nodes of mice and rats PMID: 19875849
    2. CaD stabilizes the F-actin dynamics in spines, thereby enlarging the spine heads, whereas CaD knockdown decreases the spine-head size via destabilization of the F-actin dynamics. PMID: 23077044
    3. caldesmon is an important regulator of axon development. PMID: 22158623
    4. Overexpression of l-CaD suppressed cell growth and survival in VSMCs and inhibited neointimal formation after angioplasty, partly by regulating cytoskeletal tension-FAK-ERK axis. ) PMID: 16888241
    5. Caldesmon binds and stabilizes actin filaments, as well as regulates actin-myosin interaction in a calcium (Ca2+)/calmodulin (CaM)- and/or phosphorylation-dependent manner. (Review) PMID: 21350330
    6. Stretch activates myometrium via ERK, caldesmon and focal adhesion signaling PMID: 19834610
    7. ERK1/2-mediated phosphorylation of myometrial caldesmon during pregnancy and labor PMID: 12388473
    8. caldesmon was most markedly expressed in bladder smooth muscle after partial outlet obstruction PMID: 12757606
    9. The smaller C-terminal region contains the minimum regulatory domain responsible for the inhibition of actomyosin ATPase, and for the binding to actin, calmodulin and TM. PMID: 16428310
    10. Data suggest that caldesmon may play a role in the regulation of the dynamics of podosome assembly and that Ca2+/calmodulin may be part of a regulatory mechanism in podosome formation. PMID: 16595550
    11. in Rous sarcoma virus-transformed cells, changes in the balance between PAK1/2-regulated caldesmon and the Arp2/3 complex govern the formation of podosomes PMID: 17224451
    12. Caldesmon suppresses cancer cell invasion by regulating podosome/invadopodium formation. PMID: 17631293
    13. This study reports a novel molecular mechanism (caldesmon), as well as a structural basis (podosomes/rosettes), to show how p53 can act as an anti-motility/invasion/metastasis agent. PMID: 19349302

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, myofibril. Cytoplasm, cytoskeleton, stress fiber.
  • 蛋白家族：
    Caldesmon family
  • 组织特异性：
    High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
  • 数据库链接：

    KEGG: rno:25687

    STRING: 10116.ENSRNOP00000043767

    UniGene: Rn.204926