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  4. Recombinant Rat Neutrophil gelatinase-associated lipocalin (Lcn2)

Recombinant Rat Neutrophil gelatinase-associated lipocalin (Lcn2)

  • 货号：
    CSB-YP012816RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012816RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012816RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012816RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012816RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Lcn2; Neutrophil gelatinase-associated lipocalin; NGAL; Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; Lipocalin 24p3; Lipocalin-2; Siderocalin LCN2; p25
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    21-198
  • 氨基酸序列
    QDSTQNLIPA PPLISVPLQP GFWTERFQGR WFVVGLAGNA VQKERQSRFT MYSTIYELQE DNSYNVTSIL VRGQGCRYWI RTFVPSSRPG QFTLGNIHSY PQIQSYDVQV ADTDYDQFAM VFFQKTSENK QYFKVTLYGR TKGLSDELKE RFVSFAKSLG LKDNNIVFSV PTDQCIDN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis.
  • 基因功能参考文献：
    1. NGAL might be related to attenuated tubular epithelial cell damage via adjusting autophagy. PMID: 29367586
    2. The results showed that remote limb preconditioning could decrease the expression of LCN2 protein, but having no obvious effects on its receptor except the time point of 72h after cerebral ischemia. PMID: 29122651
    3. High LCN2 expression is associated with stress-induced mitochondrial dysfunction and heart failure. PMID: 29378951
    4. This study is to investigate the level of Neutrophil gelatinase-associated lipocalin (NGAL) and autophagy status during renal Ischemia/reperfusion injury. PMID: 29704511
    5. This study demonstrated that Neutrophil gelatinase-associated lipocalin and microglial activity are associated with distinct postoperative behavioral changes in rats. PMID: 27865916
    6. LCN-2 protein levels were markedly increased in the ipsilateral cortex and hippocampus after traumatic brain injury. Most LCN-2-positive cells appeared to be astrocytes. PMID: 26463963
    7. Nitric oxide level affects LCN-2 expression via regulation of LCN-2 protein stability under inflammatory condition and LCN-2 may reduce beta-cell viability by promoting apoptosis. PMID: 27233602
    8. The NGAL molecule showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair PMID: 27087673
    9. The LCN2 may be a potentially useful biomarker for lung hypoplasia in a rat and human Congenital diaphragmatic hernia (CDH). PMID: 27592368
    10. Hypertension and hyperglycemia synergize to cause incipient renal tubular alterations resulting in increased NGAL urinary excretion.NGAL urinary excretion is also increased in hypertensive-diabetic rats. PMID: 25148248
    11. This is the first report on Pla2g4c gene-deficient rats and their low susceptibility to mammary carcinogenesis by enhancing NF-kappaB/Lcn2-induced apoptosis. PMID: 26013918
    12. Epidural anesthesia was associated with an increase in the expression lipocalin-2 and a decrease in the expression of E coli DNA at pseudosurgical sites in sick but not healthy rats. PMID: 25782996
    13. Repeated administration of gentamicin to male SD rats for 1, 3, or 7 days resulted in a dose- and time-dependent increase in the expression of KIM-1 and NGAL. PMID: 25087119
    14. dietary fructose leads to an overexpression of lipocalin-2 in rat fatty liver PMID: 24587658
    15. Lcn2 is an important regulator in small-for-size fatty liver graft injury and targeting Lcn2 may be feasible for preventing marginal graft failure in LDLT. PMID: 24374540
    16. NGF-treated tissue showed increased expression of LCN2 which was inhibited by the presence of Ro 08-2750 in intervertebral disk cells. PMID: 25062286
    17. lipocalin-2 is released by injured neurons as a help me distress signal that activates microglia and astrocytes into potentially prorecovery phenotypes. PMID: 24916903
    18. r24p3-R/hNGAL-R may represent a high-affinity multi-ligand receptor for apical internalization and transcytosis of intact proteins/peptides by the lower intestine. PMID: 23940770
    19. Urinary KIM-1, NGAL and NAG were compared against serum creatinine and renal histological score in the initiation, maintenance, and recovery phases of cisplatin (CP)-induced acute kidney injury. PMID: 23360846
    20. We studied Lcn2 gene expression during liver regeneration after partial hepatectomy(PH). Lcn2 gene expression showed upward curves from 4 to 72 h after PH in both 70% and 40% PH rats and peaked at 12 h (8 times vs 0 h). PMID: 23481292
    21. The expression of NGAL significantly increases in renal interstitial fibrosis, and is closely correlated with MMP-9, TNF-alpha and TGF-beta1. PMID: 23158800
    22. lcn2 is induced in activated astrocytes in response to neurodegeneration, but its induction is independent of TDP-43 or FUS expression in astrocytes PMID: 23431168
    23. High NGAL is associated with proteinuria. PMID: 21551085
    24. Acute myocardial infarct in the chronic kidney disease setting is associated with accelerated renal fibrosis and long-term elevated urine Ngal values. PMID: 22889806
    25. Exposure of Wistar rats to methyl tertiary-butyl ether in drinking water resulted in limited renal changes in male rats suggestive of alpha(2u)-globulin nephropathy. PMID: 22833177
    26. Adoptive transfer of IL-10 macrophages to ischemic kidneys blunts acute kidney injury through induction of lipocalin-2. PMID: 22278021
    27. LIPOCALIN-2 is a major acute-phase protein in a rat and mouse model of sterile abscess PMID: 22249220
    28. Lcn2-induced cardiomyocyte apoptosis is of physiological significance and occurs via a mechanism involving elevated intracellular iron levels and Bax translocation. PMID: 22117066
    29. An evaluation of the SIP24 and MPO distribution and levels of expression in the rat uterus is consistent with the hypothesis that SIP24 modulates the neutrophil population by activities such as inducing their apoptosis. PMID: 16546827
    30. SMC-produced NGAL interacts with MMP-9, a mechanism by which NGAL may modulate MMP-9 proteolytic activity in the vascular repair process.[Neutrophil gelatinase-associated lipocalin, NGAL] PMID: 17148685
    31. LCN2 expression shows clear correlation to liver damage and resulting inflammatory responses, rather than to the degree of liver fibrosis, which in fact may imply a distinct diagnostic value as an early biomarker of liver inflammation. PMID: 21457438
    32. Cardiomyocytes, vascular wall cells and fibroblasts in myocarditis strongly express Lcn2/NGAL via proinflammatory cytokines PMID: 20057160
    33. Acute phase response to acute endotoxemia involves induction of Lcn2 in both the lung and liver. PMID: 17490638

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  • 亚细胞定位：
    Secreted. Cytoplasmic granule lumen. Cytoplasmic vesicle lumen.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Detected in the ureteric bud in embryonic kidney (at protein level).
  • 数据库链接：

    KEGG: rno:170496

    STRING: 10116.ENSRNOP00000018776

    UniGene: Rn.11303