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  4. Recombinant Rat Natural resistance-associated macrophage protein 2 (Slc11a2), partial

Recombinant Rat Natural resistance-associated macrophage protein 2 (Slc11a2), partial

  • 货号：
    CSB-YP021381RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021381RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021381RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021381RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021381RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Slc11a2
  • Uniprot No.：
  • 别名：
    Slc11a2; Dct1; Dmt1; Nramp2Natural resistance-associated macrophage protein 2; NRAMP 2; Divalent cation transporter 1; Divalent metal transporter 1; DMT-1; Solute carrier family 11 member 2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May serve to import iron into the mitochondria. Important in metal transport, in particular iron. Can also transport zinc, manganese, cobalt, cadmium, copper, nickel and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.
  • 基因功能参考文献：
    1. DMT1 mediates mitochondrial Fe2+ and Mn2+ acquisition. PMID: 29317744
    2. impaired intestinal expression of Dcyt-b and DMT-1 might be associated with the reduction of an iron uptake in CKD. Taken together, impaired these intestinal iron transporters may become a novel therapeutic target for cardio-renal anemia syndrome. PMID: 28669019
    3. Mutations in the divalent metal transporter 1 (DMT1) decrease body iron status and up-regulate copper absorption PMID: 27331785
    4. DMT1 and FPN1 are positively influenced by ferrous iron status in brain after intracerebral hemorrhage. DMT1 and FPN1 attenuate iron overload after ICH via increasing transmembrane iron export. PMID: 26617777
    5. work unveils the role of DMT1 in mediating the neuroregenerative action of iron. PMID: 26360295
    6. These results suggest that the genetic ablation of iPLA2b increased iron uptake in the brain through the activation of IRP2 and upregulation of DMT1, which may be associated with mitochondrial dysfunction. PMID: 26506412
    7. the effects of hepcidin expression and hepcidin peptide on brain iron contents, iron transport across the brain-blood barrier, iron uptake and release, and also the expression of TfR1, DMT1, and Fpn, were investigated. PMID: 25115800
    8. DMT1 expression was enhanced in the bone tissue of type 2 diabetic rats, and plays an important role in the pathological process of diabetic osteoporosis. PMID: 26078704
    9. Goat milk consumption potentiates liver DMT1 expression, enhancing Fe metabolism and storage. PMID: 24239078
    10. Hepcidin or enterocyte iron levels may be involved in the regulation of the age-dependent expression in the duodenum. PMID: 25318588
    11. Spinal peroxynitrite activates DMT1-iron responsive element, leading to abnormal iron accumulation in postoperative hyperalgesia. PMID: 25501899
    12. Data suggest that expression of Dmt1/Slc11a2 in intestinal mucosa can be regulated by dietary factors; here, expression of Dmt1/Slc11a2 in mucosa of cecum is up-regulated by prebiotic supplement, inulin, in iron-deficiency anemia. PMID: 25745840
    13. All three isoforms of DMT1 are selectively expressed in different cell populations within the cochlea and, additionally, demonstrate their cellular and subcellular distribution changes with development. PMID: 24318355
    14. Dmt1 expression was significantly elevated through pro-inflammatory cytokines, whereas Ft expression was marginally increased. PMID: 24850829
    15. DMT1 protein levels are down-regulated in iron-loaded liver and up-regulated in iron-deficient liver and heart. PMID: 23349308
    16. DMT1 not only exports iron from endosomes, but also serves to import the metal into the mitochondria PMID: 24448823
    17. DMT1 is a candidate for non-transferrin-bound iron uptake within the peripheral nervous system. PMID: 23361852
    18. The data presented here establish for the first time a causal association between inflammation and iron accumulation in brain cells, probably promoted by changes in DMT1 and FPN1 expression and mediated in part by hepcidin. PMID: 23506423
    19. Whole-genome profiling reveals that increasing dietary protein from 5 to 40% increases duodenal transcript expression of divalent metal transporter 1 (DMT1) 3.2-fold, duodenal cytochrome b (Dcytb) 1.8-fold, and transferrin receptor (TfR) 1.8-fold. PMID: 23447582
    20. analysis of synthesis and biological evaluation of substituted pyrazoles as blockers of divalent metal transporter 1 (DMT1) PMID: 22154351
    21. These data indicate that CTR1, but not DMT1, plays an essential role in transporting Cu by the blood-CSF barrier in the choroid plexus PMID: 22465424
    22. these results suggest that Ctr1, DMT1, ATOX1 and ATP7A contribute to Cu transport at the blood-CSF barrier PMID: 22442359
    23. Pulmonary inflammation can be modified by both DMT1 and iron status. PMID: 21278260
    24. DMT1+IRE up-regulation can account for IRE/IRP-dependent 6-OHDA-induced iron accumulation initiated by 6-OHDA-induced intracellular oxidative stress and that increased levels of intracellular iron result in aggravated oxidative stress. PMID: 20125122
    25. Suggest a novel mechanism of regulation of intestinal iron absorption based on inward and outward fluxes at both membrane domains, and repositioning of DMT1 and FPN between membrane and intracellular compartments as a function of iron supply. PMID: 20007457
    26. Divalent metal transporter-1 is present on astrocytic end feet in contact with blood vessels, suggesting that it may be involved in uptake of iron from endothelial cells. PMID: 12429222
    27. results demonstrated that DMT1 mRNAs expression in the heart is age-dependent and that two forms of DMT1 mRNAs both are regulated by iron on the post-transcriptional level only PMID: 12767048
    28. modulation of renal DMT1 expression by dietary iron intake may influence renal iron excretion rate. PMID: 12876064
    29. in iron deficiency DMT-1 and mobilferrin concentrated in apical surface of villae. increase due to increased binding to mucin in vesicles near surface; localized in goblet cells and outside cell in luminal mucin. PMID: 12949888
    30. At day 20, DMT1 increased fourfold and FPN1 increased eightfold in the low-Fe group. Molecular mechanisms regulating iron absorption during early infancy differ from late infancy. PMID: 12958019
    31. G185R mutation of DMT1 causes protein instability in the kidneys of b/b anemic rats. PMID: 14531808
    32. Brain iron transport and distributional pattern of divalent metal transporter I (DMT1) were studied in homozygous Belgrade rats (b/b) which suffer from a mutation in the DMT1 gene; low cerebral iron uptake may derive from a reduced neuronal uptake PMID: 14675167
    33. DMT-1 does not play a major role in brain manganese uptake; one or more carrier-mediated processes other than that of DMT-1 are primarily involved. PMID: 15019308
    34. DMT1 may play a role in transporting iron between intracellular compartments in spermatogenic epithelium and thus may play an important role in male fertility. PMID: 15355847
    35. high-resolution structures of a synthetic peptide, corresponding to the sequence of the fourth transmembrane domain of rat DMT1 with G185D mutation PMID: 15660380
    36. Immunofluorescent staining of thin sections of proximal intestines of b/b and +/b Belgrade rats confirms that DMT1 localizes similarly in mutant and control enterocytes and shows that DMT1 isoforms have distinct distributions within intestinal tissue. PMID: 15736955
    37. Primary role for DMT1 in lung metal transport and detoxification. PMID: 15908475
    38. Ferroportin antibody induced rapid turnover of membrane ferroportin & DMT1 & its internalisation to late endosomes, resulting in impaired Fe(II) uptake. PMID: 16075245
    39. The molecular mechanism of iron transport by wild type and mutant DMT1 is reported. PMID: 16091957
    40. renal proximal tubular cells express divalent metal transporter 1 (DMT1) in the membranes of organelles, including late endosomes/lysosomes, associated with processing of apically sequestered transferrin PMID: 16449358
    41. Exposure of P19 cells to a NO precursor, resulted in a decrease in expression of both positive and negative IRE isoforms of DMT1 with no change in the 1A species. PMID: 16539692
    42. Brain capillary endothelial cells probably mediate iron transport into the brain by segregating iron from transferrin without involvement of DMT1 PMID: 16879716
    43. This study demonstrated that endosomal/lysosomal DMT1 plays a role in renal proximal tubule cadmium-methallothionein1 toxicity. PMID: 17596526
    44. DMT1 function is not essential for iron assimilation from milk during early development in the rat. PMID: 17640977
    45. rHuEpo injection reduced the peritoneal exudate macrophages iron retention. The uptake of Fe(II) was decreased via the suppression of DMT1 (+IRE) expression and the release of Fe(II) was increased with increasing the expression of FPN1 in macrophages. PMID: 18189270
    46. Our findings also show that MPP(+)-induced apoptosis in MES23.5 cells involves DMT1-dependent iron influx and mitochondria dysfunction. PMID: 18191877
    47. An increase in HO2 and DMT1 mRNA level was induced after chronic exposure to depleted uranium. PMID: 18375546
    48. The up-regulation of DMT1-IRE and the increase in DMT1-IRE-mediated iron influx play a key role in L-DOPA neurotoxicity in cortical neurons PMID: 19240805

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  • 相关疾病：
    Defects in Slc11a2 are the cause of microcytic anemia (Belgrade or b). Homozygous b/b rats have hypochromic microcytic anemia due to severe defects in intestinal iron absorption and erythroid iron utilization.
  • 亚细胞定位：
    Endosome membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    NRAMP family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: rno:25715

    STRING: 10116.ENSRNOP00000026531

    UniGene: Rn.11418