Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat NAD (P)H dehydrogenase [quinone] 1 (Nqo1)

Recombinant Rat NAD (P)H dehydrogenase [quinone] 1 (Nqo1)

  • 中文名称：
    大鼠Nqo1重组蛋白
  • 货号：
    CSB-YP016039RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Nqo1重组蛋白
  • 货号：
    CSB-EP016039RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Nqo1重组蛋白
  • 货号：
    CSB-EP016039RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Nqo1重组蛋白
  • 货号：
    CSB-BP016039RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Nqo1重组蛋白
  • 货号：
    CSB-MP016039RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nqo1
  • Uniprot No.：
  • 别名：
    Nqo1; Nmor1; NAD(P)H dehydrogenase [quinone] 1; EC 1.6.5.2; Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-274
  • 氨基酸序列
    AVRRALIVL AHAERTSFNY AMKEAAVEAL KKKGWEVVES DLYAMNFNPL ISRNDITGEP KDSENFQYPV ESSLAYKEGR LSPDIVAEQK KLEAADLVIF QFPLYWFGVP AILKGWFERV LVAGFAYTYA TMYDKGPFQN KKTLLSITTG GSGSMYSLQG VHGDMNVILW PIQSGILRFC GFQVLEPQLV YSIGHTPPDA RVQVLEGWKK RLETVWEESP LYFAPSSLFD LNFQAGFLLK KEVQEEQKKN KFGLSVGHHL GKSIPADNQI KARK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • 基因功能参考文献：
    1. Data indicate that non-CpGI promoters exert important functions in the epigenetic regulation of NAD(P)H:quinone oxidoreductase 1 (NQO1). PMID: 29889218
    2. Downregulation of NQO1 promotes inflammatory response in lung tissues of sepsis-associated acute respiratory distress syndrome. PMID: 30121199
    3. Results support the protective role of DT-diaphorase against aminochrome-induced neurotoxicity. Specifically, DT-diaphorase prevents autophagy inhibition by preventing the formation of aminochrome-induced SNCA oligomers. PMID: 28478529
    4. Study shows that the distribution of NAD(P)H quinone dehydrogenase 1 (NQO1) olfactory neurons in rats recapitulates that in mice. Hydrogen Sulfide toxicity specifically affects NQO1 olfactory neuron expression. The present findings may be used to further study the function of these neurons. PMID: 29054567
    5. Collectively, the results suggest that beta-LAP increases antioxidant enzyme gene expression in astrocytes by modulating NQO1-AMPK/PI3K-Nrf2/ARE signaling. PMID: 27242267
    6. These results, together with the findings that the protein contents of DLDH, sirt3, and NQO1 all are decreased in the diabetic lung, demonstrate that redox imbalance, mitochondrial abnormality, and oxidative stress contribute to lung injury in diabetes. PMID: 27888691
    7. eNOS acetylation can be regulated by NQO1 activation in an SIRT1-dependent manner, which is correlated with the relief of hypertension. PMID: 24951727
    8. results indicate that AAI can increase its own metabolic activation by inducing NQO1, thereby enhancing its own genotoxic potential. PMID: 24769487
    9. NAD(P)H:quinone oxidoreductase-1 activity and increase of hydrogen peroxide production by NADPH oxidases are early biomarkers in doxorubicin cardiotoxicity. PMID: 24506563
    10. In some nuclei (nucleus ambiguus, hypoglossal nerve nucleus), containing numerous NADPH-diaphorase-positive neurons, immunoreactive cells were particularly rare PMID: 24707733
    11. NQO1 involved in the imine bond reduction of sanguinarine and this was a less toxic metabolizing pathway than CYP1A1-metabolizing pathway. PMID: 24300172
    12. Activity of retinal afferents influences development and maintenance of NADPH-diaphorase-positive neurons in retinorecipient layers of the superior colliculus, where nitric oxide acts as a retrograde signal carving the terminal arbors of retinal axons. PMID: 23420945
    13. Elevated p53 mediated glucolipotoxicity-induced apoptosis of rat cardiomyoblast cell through dual pathways: stimulating pro-apoptosis signaling as well as suppressing anti-apoptosis pathway of Nrf2-NQO1 signaling. PMID: 22154326
    14. In adults, the NADPH-d positivity was detectable in the neurons of superficial and deep layers of the dorsal horn, pericentral area and in the area of preganglionic autonomic nuclei. PMID: 22028090
    15. jejunal ischemia/reperfusion has affected the neurons of the enteric nervous system of adult rats and resulted in the early decrease of NADPH-d positivity 1 h of the reperfusion insult. PMID: 22073370
    16. NQO1 and heme oxygenase 1 (HO-1) play a critical role in antioxidant effects of isoflavone metabolites in rat brain astrocytes. PMID: 21781119
    17. Carnosic acid and carnosol induced NQO1 protein expression in a dose-dependent manner. PMID: 22031657
    18. NQO1 activation has a hypotensive effect mediated by eNOS activation via cellular NAD(+)/NADH ratio modulation in an animal model. PMID: 21502369
    19. The nuclear factor Nrf2 target, NAD(P)H:quinone oxidoreductase (NQO1), exhibits increased activity in both hippocampus and liver after 3 weeks of a ketogenic diet. PMID: 20594978
    20. NAD(P)H:quinone oxidoreductase 1 (NQO1) Pro187Ser polymorphism is associated with colorectal cancer. PMID: 20593958
    21. The protection exerted by sulforaphane(SFN) on mitochondrial alterations and NQO1 and gammaGCL enzymes may be involved in the renoprotection of SFN against cisplatin. PMID: 20732396
    22. Expression of c-Fos and NADPH-d was induced in the related brainstem during vestibular compensation. PMID: 19180910
    23. The expression and distribution of NADPH-d and HO-2 suggest that carbon monoxide and nitric oxide are likely neurotransmitters and might function in the processing orofacial signal in the spinal trigeminal nucleus together. PMID: 19821077
    24. Dynamics of NADPH diaphorase activity in raphe neurons during chronic treatment with opiates PMID: 11740593
    25. Exposure to diesel exhaust particles elevated transcription of NADPH dehydrogenase gene in rat alveolar macrophages. PMID: 12011483
    26. distribution of Dia4 in rat mesencephalon PMID: 12240559
    27. Induction of NQO1 activity exaggerates neuronal damage in cultured oxygen-glucose deprived rat cortical neurons PMID: 12603827
    28. NADPH diaphorase co-localizes with Nos1 and NMDAR1 in the nodose ganglion (NG) under normoxic and hypoxic conditions: both the percentage and staining of immunoreactive neurons in the NG are drastically increased following hypoxic exposure. PMID: 12663061
    29. complete loss of NADPH-d-positive neurons in the CA1 and CA3 subfields and also in the hilus of dentate gyrus was observed after the administration of dopamine PMID: 12699333
    30. After 6 weeks of diabetes, NADPH diaphorase amacrine cells in the diabetic retinas appeared abnormal in their morphology and the degree of staining was decreased in their processes. In contrast, NADPH diaphorase activity was augmented in Muller cells. PMID: 14564531
    31. we demonstrate that blood vessel NADPH-diaphorase staining peaked immediately after the hypoxic stimulus. PMID: 14980808
    32. NQO1 is localized to Bergmann glia in the cerebellum and a subset of the oligodendrocytes throughout the brain, and a role is suggested for NQO1 in myelination in the central nervous system or in the insulating/wrapping function of glial cells. PMID: 14991562
    33. Both high neuropil reactivity and differential distribution of NADPH-d neurons during somatosensory cortex development suggest a role for nitric oxide throughout barrel field maturation PMID: 15288499
    34. These results suggested that hypoxic preconditioning might have a neuroprotective effect that reduces the susceptibility of the nodose neurons to NOS mediated neuropathy subsequent to hypoxia PMID: 15652701
    35. Systematic qualitative and quantitative analysis of NADPH-diaphorase-positive cells was performed in the neonatal (P14), adult(5 months) and aging (20 months) rat Rostral Migratory Stream. PMID: 16392039
    36. NADPH-diaphorase neurons in the visual cortex are resistant to arginine and nutritional changes. PMID: 16426587
    37. The major distribution of the NADPH-d positive bodies in the aged lumbosacral spinal cord indicates some anomalous changes in the neurite, which might account for a disturbance in the aging pathway of the autonomic and sensory nerve in the pelvic organs. PMID: 17372386
    38. Colocalization of somatostatin and NADPH-d with somatostatin receptor subtypes in hypothalamic regions was similar, suggesting that somatostatin and NADPH-d producing cells are same. PMID: 17626271
    39. Nqo1 protein expression and activity underwent significant upregulation in methionine and choline-deficient rat liver but not in high-fat rat liver. PMID: 17721935
    40. The highly expressed and inducible endogenous NQO1 in cardiovascular cells may act as a potential O2(.-) scavenger. PMID: 17901563
    41. Report structure-activity relationships and organ specificity in the induction of GST and NQO1 by alkyl-aryl isothiocyanates. PMID: 18563540
    42. NADPH-diaphorase and heme oxygenase-2 positive neurons appeared the same pattern PMID: 18626777
    43. Activation of NAD(P)H:quinone oxidoreductase 1 prevents arterial restenosis by suppressing vascular smooth muscle cell proliferation. PMID: 19229058
    44. Results suggest that NQO1 can contribute to inhibition of colon carcinogenesis at the post-initiation stage. PMID: 19424637
    45. NADPH-d activity in the rat medial amygdala is different between sexes but in females it is not affected by changing levels of circulating gonadal hormones in physiological or supraphysiological conditions. PMID: 19815007

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    NAD(P)H dehydrogenase (quinone) family
  • 数据库链接：

    KEGG: rno:24314

    STRING: 10116.ENSRNOP00000017175

    UniGene: Rn.11234