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  4. Recombinant Rat N-glycosylase/DNA lyase (Ogg1)

Recombinant Rat N-glycosylase/DNA lyase (Ogg1)

  • 货号：
    CSB-YP016313RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016313RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016313RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016313RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016313RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ogg1; Mmh; Ogh1; N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase; EC 3.2.2.-); DNA-(apurinic or apyrimidinic site) lyase; AP lyase; EC 4.2.99.18)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-345
  • 氨基酸序列
    MLFSSSLSSS MRHRTLTSSP ALWASIPCPR SELRLDLVLA SGQSFRWREQ SPAHWSGVLA DQVWTLTQTE DQLYCTVYRG DKGQVGRPTL EELETLHKYF QLDVSLTQLY SHWASVDSHF QSVAQKFQGV RLLRQDPTEC LFSFICSSNN NIARITGMVE RLCQAFGPRL VQLDDVTYHG FPNLHALAGP EVETHLRKLG LGYRARYVCA SAKAILEEQG GPAWLQQLRV ASYEEAHKAL CTLPGVGTKV ADCICLMALD KPQAVPVDIH VWQIAHRDYG WQPKTSQTKG PSPLANKELG NFFRNLWGPY AGWAQAVLFS ADLRQQNLSR EPPAKRKKGS KKTEG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
  • 基因功能参考文献：
    1. chronic hypobaric hypoxia attenuates OGG1 expression in rat spermatozoa in a time-dependent fashion, with a greater reduction in the mitochondrial isoform OGG1-2a. PMID: 28758699
    2. The increase in the oxidative stress in compensated cardiac hypertrophy induced the OGG1-2a migration to mitochondria to repair mtDNA oxidation, as a mechanism that allows maintaining the cardiac function in the compensatory stage. PMID: 28882450
    3. Ogg1 overexpression also reduced binding of mitochondrial transcription factor A (TFAM) to both regulatory and coding regions of the mitochondrial genome without altering total abundance of TFAM in either control or hypoxic cells. These observations suggest that oxidative DNA modifications in the D-loop region during hypoxia are important for increased TFAM binding and ensuing replication of the mitochondrial genome. PMID: 27091693
    4. Oxidative DNA damage occurred in lung epithelial cells during earlystage bronchopulmonary dysplasia, as confirmed by in vitro and in vivo hyperoxia exposure experiments, and the increased expression of OGG1 was associated with this process. PMID: 25672835
    5. glucose induces apoptosis and inhibits proliferation of glial cells, which may be mediated by the phosphorylation of tuberin, down regulation of 8-oxoG-DNA glycosylase and 8-hydroxydeoxyguanosine accumulation via activation of Akt and ERK1/2MAPK pathways PMID: 24907397
    6. high-running capacity is associated with increased hippocampal function, and SIRT1 level/activity and inversely correlates with acetylated Ogg1 levels PMID: 23973402
    7. Our studies suggest that antioxidants provide protection against oxidative DNA damage and E2-induced mammary carcinogenesis, at least in part, through NRF2-mediated induction of OGG1. PMID: 23697596
    8. these results suggest that DNA glycosylases OGG1 and NEIL3 play multiple roles in governing essential neural stem cell characteristics. PMID: 22564741
    9. The upregulation of base-excision repair activities after ischemic preconditioning was likely because of increased expression of OGG1 protein. PMID: 16001017
    10. Estrogens differentially regulate expression of 8-oxoguanine DNA glycosylase, but not of Kin17 protein, in specific regions of rat brain. Estradiol can translocate 8-oxoguanine DNA glycosylase protein within nuclei and to other subcellular compartments. PMID: 16182450
    11. These results demonstrate that OGG1 localizes with tuberin preferentially in kidney cortex. Loss of tuberin is accompanied by the loss of OGG1 contributing to tumorigenesis. PMID: 18218111

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  • 亚细胞定位：
    Nucleus, nucleoplasm. Nucleus speckle. Nucleus matrix.
  • 蛋白家族：
    Type-1 OGG1 family
  • 数据库链接：

    KEGG: rno:81528

    STRING: 10116.ENSRNOP00000011352

    UniGene: Rn.22623