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  4. Recombinant Rat N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (Napepld)

Recombinant Rat N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (Napepld)

  • 中文名称：
    大鼠Napepld重组蛋白
  • 货号：
    CSB-YP762319RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Napepld重组蛋白
  • 货号：
    CSB-EP762319RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Napepld重组蛋白
  • 货号：
    CSB-EP762319RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Napepld重组蛋白
  • 货号：
    CSB-BP762319RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Napepld重组蛋白
  • 货号：
    CSB-MP762319RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Napepld
  • Uniprot No.：
  • 别名：
    Napepld; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; N-acyl phosphatidylethanolamine phospholipase D; NAPE-PLD; NAPE-hydrolyzing phospholipase D; EC 3.1.4.54
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-396
  • 氨基酸序列
    MDENENSQSP APSHQYPKET LRKRQNSVQN SGGSESSRLS RKSFKLDYRL EEDVTKSKKG KDGRFVNPWP TWKNVSIPNV LRWLIMEKDH SSVPGSKEEL DKELPVLKPY FISDPEEAGV REAGLRVTWL GHATLMVEMD ELILLTDPMF SSRASPSQYM GPKRFRRPPC TISELPPIDA VLISHNHYDH LDYGSVLALN ERFGSELRWF VPLGLLDWMQ KCGCENVIEL DWWEENCVPG HDKVTFVFTP SQHWCKRTLL DDNKVLWGSW SVLGPWNRFF FAGDTGYCPA FEEIGKRFGP FDLAAIPIGA YEPRWFMKYQ HADPEDAVRI HIDVQAKRSV AIHWGTFALA NEHYLEPPVK LNEALERYGL KSEDFFILKH GESRYLNTDD KAFEET
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs.
  • 基因功能参考文献：
    1. These results demonstrated wide distribution of NAPE-PLD in various brain regions and its age-dependent expression, suggesting the central role of this enzyme in the formation of anandamide and other N-acylethanolamines in the brain. PMID: 15992380
  • 亚细胞定位：
    Golgi apparatus membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Nucleus envelope. Nucleus, nucleoplasm.
  • 蛋白家族：
    NAPE-PLD family
  • 组织特异性：
    Widely expressed. Highest expression in brain, kidney and testis (at protein level). Expressed in adipose tissue (at protein level).
  • 数据库链接：

    KEGG: rno:296757

    STRING: 10116.ENSRNOP00000015322

    UniGene: Rn.101892