Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Metallothionein-3 (Mt3)

Recombinant Rat Metallothionein-3 (Mt3)

  • 货号：
    CSB-YP015122RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015122RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015122RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015122RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015122RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mt3
  • Uniprot No.：
  • 别名：
    Mt3; Metallothionein-3; MT-3; Growth inhibitory factor; GIF; Metallothionein-III; MT-III
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-66
  • 氨基酸序列
    MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV CKGEEGAKAE KCSCCQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.
  • 基因功能参考文献：
    1. After passing middle age we observe a huge increase in MT3 transcripts in both cortical and hippocampal areas, while the MT1/2 mRNA content increases slightly PMID: 27939232
    2. Neuroprotective effect of propofol in hypoxia-reoxygenation injury of hippocampal neurons may be via upregulation of Mt3. PMID: 22367221
    3. metallothionein III, in the presence of brain extract, is a potent inhibitor of neurite sprouting, and may be involved in abnormal sprouting potentially underlying both Alzheimer's disease and epilepsy. PMID: 12460603
    4. Systemic application of kainic acid (KA) rapidly induced MT-I and II expression in neurons localized in hippocampal formation, piriform cortex, and amygdala of the adult rat, whereas the level of MT-III mRNA was decreased in KA-vulnerable areas PMID: 12692462
    5. A recombinant adenoviral vector encoding GIF prevents degeneration of motoneurons in rats after facial nerve avulsion. PMID: 14625437
    6. differential display proteomics techniques identified ten significantly and reproducibly changed proteins induced by transient transfection of MT-3 in PC-12 cells; their functional implications are discussed PMID: 14998173
    7. A large amount of free zinc ions can be sequestered by metallothionein-3 in the hippocampus of old rats, leading to impaired zinc-dependent functions in the ageing brain. PMID: 15130702
    8. analysis of the epitope of neuronal growth inhibitory factor (GIF) PMID: 16336778
    9. mutation of MT3 at Glu23 may alter the NO metabolism and/or affect zinc homeostasis in brain, thus altering the neuronal growth inhibitory activity PMID: 16945328
    10. The localization of MT-3 in peripheral organs outside the brain suggests that MT-3 has roles in these tissues besides its role in growth inhibition of neurites. PMID: 18479819

    显示更多

    收起更多

  • 蛋白家族：
    Metallothionein superfamily, Type 1 family
  • 组织特异性：
    Brain.
  • 数据库链接：

    KEGG: rno:117038

    STRING: 10116.ENSRNOP00000025669

    UniGene: Rn.11325