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  4. Recombinant Rat Indoleamine 2,3-dioxygenase 1 (Ido1)

Recombinant Rat Indoleamine 2,3-dioxygenase 1 (Ido1)

  • 货号：
    CSB-YP010996RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010996RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010996RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010996RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010996RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ido1; Ido; Indo; Indoleamine 2,3-dioxygenase 1; IDO-1; EC 1.13.11.52; Indoleamine-pyrrole 2,3-dioxygenase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-407
  • 氨基酸序列
    MPHSQISPAE GSRRILEEYH IDEDVGFALP HPLEELPDTY RPWILVARNL PKLIENGKLR EEVEKLPTLR TEELRGHRLQ RLAHLALGYI TMAYVWNRGD DDIRKVLPRN LAVPYCELSE KLGLPPILSY ADCVLANWKK KDPNGPMTYE NMDILFSFPG GDCDKGFFLV SLMVEIAASP AIKAIPTVSS AVEHQDPKAL EKALCSIAAS LEKAKEIFKR MRDFVDPDTF FHVLRIYLSG WKGNPKLPEG LLYEGVWDTP KKFSGGSAGQ SSIFQSLDVL LGIKHDVGEG SAAEFLQEMR EYMPPAHRNF LSSLESAPPV REFVILRRNE DLKEAYNECV NGLVSLRMFH LSIVDTYIVK PSKQKPMGGH KSEEPSNTEN RGTGGTDVMN FLRSVKDTTK KALLSWP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection.
  • 基因功能参考文献：
    1. Results show the antidepressant effect of infliximab and suggest that its mechanism is partly related to inhibition of indoleamine 2, 3-dioxygenase/3-hydroxyl amino acid oxygenase pathway activation mediated by tumor necrosis factor-alpha in rat brain. PMID: 27366867
    2. this study suggests a link between melatonin, JNK, FoxO1 and IDO1 that acts as a potential balance regulator of tryptophan metabolism, and offers a new approach to treat diseases related to dysregulation of tryptophan metabolism PMID: 28118532
    3. results suggest that M30 is neuroprotective against CORT-induced depression targeting elevated MAO activities that cause oxidative stress and neuroinflammation, resulting in IDO-1 activation, serotonin deficiency and neurodegeneration. PMID: 27870896
    4. IDO expression in NSCs synergistically potentiates the immune suppression activities of NSCs and could be applicable for the development of therapeutic modalities against various neurodegenerative diseases. PMID: 26636969
    5. study demonstrates that IDO gene transfer into engineered lung allograft tissues significantly attenuates acute allograft damage suggesting local therapy with IDO as a strategy to reduce need for systemic immunosuppression and, thereby, its side effects PMID: 26506443
    6. Indoleamine 2,3-dioxygenase is upregulated in the brain of rats with acute pancreatitis. PMID: 25829217
    7. inhibitory effect of locally delivered CTLA4Ig is dependent on IDO activities within the allograft. PMID: 25498102
    8. Transfer of tolerance to naive recipients is dependent upon IDO-mediated immunosuppression. Initiators of IDO activity, CTLA-4 or soluble CTLA-4 did not mediate this tolerogenic process. PMID: 23607691
    9. results suggest IDO gene expression correlates with the severity of acute rejection and IFN-gamma induced IDO-positive DCs may attenuate acute rejection and catalyze local tryptophan metabolism via IDO enzyme expression,leading to tolerance after liver transplantation PMID: 22454246
    10. Ido1 gene knockout or pharmacological inhibition of hippocampal IDO1 activity attenuated both nociceptive and depressive behavior PMID: 22751107
    11. zebularine plus interferon gamma induce IDO1 gene expression related to demethylation events in the IDO1 promoter region and to up-regulation of expression of transcription factors PMID: 22424783
    12. Detection of IDO mRNA in peripheral blood diagnosed acute rejection in the rat model at an earlier stage. PMID: 22172881
    13. Data suggest that during the first phase of infection the IDO-mediated tryptophan depletion has a predominantly antimicrobial effect. PMID: 19602041
    14. NK cells expression is associated with liver allograft acceptance PMID: 19944758
    15. HO-1/IDO cross-regulation modulates apoptosis and proliferation in rat and human breast cancer cells PMID: 16319139
    16. In conclusion, this study demonstrates an age-related decline in Cu/Zn-SOD and IDO activities, the two enzymes responsible for scavenging O2*-. PMID: 16688932
    17. Report immunological role of indoleamine 2,3-dioxygenase in rat liver allograft rejection and tolerance. PMID: 17645734
    18. CD4+T cells accumulate at the heart transplant site and induce graft endothelial cell expression of Indoleamine 2, 3-dioxygenase (IDO) PMID: 17868070
    19. IDO inhibition for 7 days after transplantation reduces survival, but does not cause acute rejection of the liver in an immunotoleramt model. PMID: 18475196
    20. gene delivery using IDO was unsuccessful in prolonging rat liver allograft survival. PMID: 19177450
    21. In vitro, the proliferative ability of T cells or B cells from experimental autoimmune myastenia gravis rats was inhibited when they were cocultured with bone marrow stromal cells. This inhibitory effect was partially dependent on the secretion of IDO PMID: 19283707
    22. Liver-induced long-term acceptance seen in human combined auxiliary liver and kidney transplantation is at least partly mediated by InDO activity. PMID: 19935463

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Indoleamine 2,3-dioxygenase family
  • 数据库链接：

    KEGG: rno:66029

    STRING: 10116.ENSRNOP00000050320

    UniGene: Rn.64513