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  4. Recombinant Rat Guanylate cyclase soluble subunit beta-1 (Gucy1b3)

Recombinant Rat Guanylate cyclase soluble subunit beta-1 (Gucy1b3)

  • 货号：
    CSB-YP010052RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010052RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010052RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010052RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010052RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gucy1b1; Guc1b3; Gucy1b3Guanylate cyclase soluble subunit beta-1; GCS-beta-1; EC 4.6.1.2; Guanylate cyclase soluble subunit beta-3; GCS-beta-3; Soluble guanylate cyclase small subunit
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-619
  • 氨基酸序列
    MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD LVAAASKVLN LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN LDALHDHLAT IYPGMRAPSF RCTDAEKGKG LILHYYSERE GLQDIVIGII KTVAQQIHGT EIDMKVIQQR SEECDHTQFL IEEKESKEED FYEDLDRFEE NGTQDSRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL PQLQPGKCSL LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGAE ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR DLVLLGEQFR EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP SVANELRHKR PVPAKRYDNV TILFSGIVGF NAFCSKHASG EGAMKIVNLL NDLYTRFDTL TDSRKNPFVY KVETVGDKYM TVSGLPEPCI HHARSICHLA LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY CLFGNTVNLT SRTETTGEKG KINVSEYTYR CLMSPENSDP QFHLEHRGPV SMKGKKEPMQ VWFLSRKNTG TEETNQDEN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.
  • 基因功能参考文献：
    1. The effect of glucagon-like peptide-2 on the expression of the alpha1, alpha2, and beta1 subunits of soluble guanylyl cyclase, was studied. PMID: 22806360
    2. soluble GCbeta1 plays an important role in the restructuring of the adherens junction in the testis [sGCbeta1] PMID: 16547975
    3. Identify hydrogen peroxide as an endogenous mediator contributing to the regulation of vascular tone and point to a key role of HuR in sGCbeta1 mRNA stabilization. PMID: 21164076
    4. Soluble guanylate cyclase is activated by binding of carbon monoxide and YC-1. PMID: 20353168
    5. soluble guanylate cyclase beta 1 expression in the vasculature of rat skeletal muscle PMID: 20009348
    6. mapping of heme-binding domain PMID: 11688978
    7. treatment of rat brain astrocytes with betaA decreases their capacity to accumulate cyclic GMP (cGMP) in response to NO as a result of a decreased expression of soluble guanylyl cyclase alpha-1 and beta-1 subunits at the protein and mRNA levels. PMID: 12127152
    8. Expression of sGC beta 1 subunit is tightly coregulated with that of sGC alpha 1 in rat brain, while yet unknown additional regulatory mechanisms affect the Vmax of sGC. PMID: 12231239
    9. sGC alpha(1) and beta(1) subunit protein levels in the ovary during postnatal development, gonadotropin-induced follicle growth, ovulation, and luteinization as well as in cultured rat granulosa cells PMID: 14749300
    10. Results demonstrate that hypoxia decreases soluble guanylate cyclase alpha1 and beta1 expression and suggest that, in hypoxic vascular smooth muscle, decreased cyclic GMP synthesis may limit the vasodilator response to nitric oxide. PMID: 14754757
    11. Soluble guanylate cyclases could be the target molecules for controlling neo-vascularization in a subset of human malignant gliomas. PMID: 15201957
    12. Soluble guanylyl cyclase appears in a specific subset of periglomerular cells in the olfactory bulb. PMID: 15813955
    13. Results identify and characterize the minimum functional ligand-binding heme domain derived from soluble guanylate cyclase. PMID: 16331987
    14. for the function of sGC in vivo, where there is evidence for two nitric oxide-regulated activity states PMID: 17098738
    15. Suggest that in coronary arterioles of obese rats, increased activity of sGC leads to an enhanced sensitivity to NO, which may contribute to the maintenance of NO-mediated dilations and coronary perfusion in obesity. PMID: 18408126
    16. characterization of the promoter activity of human sGC alpha(1) and beta(1) subunits and identification of key transcription factors that govern subunit expression under basal and pro-inflammatory conditions and in the presence of ligand NO PMID: 18474600
    17. Estradiol physiologically regulates sGC expression and activity in rat anterior pituitary gland. PMID: 19141686
    18. beta1 is a multifunctional protein that regulates chromatin condensation and cell cycle progression, in addition to being an obligate monomer in functional nitric oxide-sensitive guanylyl cyclase heterodimers. PMID: 19433313
    19. Soluble guanylyl cyclase activity is an important element not only in neurite outgrowth but also in synapse formation and maturation in cerebellar granule cells. PMID: 19461654
    20. Report distinct molecular requirements for activation or stabilization of soluble guanylyl cyclase upon haem oxidation-induced degradation. PMID: 19466990

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Adenylyl cyclase class-4/guanylyl cyclase family
  • 组织特异性：
    Lung and brain.
  • 数据库链接：

    KEGG: rno:25202

    STRING: 10116.ENSRNOP00000062785

    UniGene: Rn.87228