Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Glycine receptor subunit alpha-1 (Glra1), partial

Recombinant Rat Glycine receptor subunit alpha-1 (Glra1), partial

  • 货号：
    CSB-YP009516RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009516RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009516RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009516RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009516RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Glra1
  • Uniprot No.：
  • 别名：
    Glra1; GlyrGlycine receptor subunit alpha-1; Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol. Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol.
  • 基因功能参考文献：
    1. gain-of-function GLRA1 mutations also cause hyperekplexia, although the mechanism is unknown. Here we identify two new gain-of-function mutations (I43F and W170S) and characterize these along with known gain-of-function mutations (Q226E, V280M, and R414H) to identify how they cause hyperekplexia. PMID: 27226610
    2. analysis of the SENP-1 mediated SUMOylation-dependent regulatory pathway for GlyR endocytosis, which may have important physiological implications for proper neuronal excitability PMID: 25236484
    3. an essential secondary structural requirement for GlyR modulation by ethanol and G protein PMID: 25339760
    4. overexpressing the Glra1 subunite in rat neurons caused reduced potentition and enhanced inhibition by zinc. PMID: 24198360
    5. Hypoxia alters the expression of the GABAA Ralpha receptors and glycine alpha1 receptor in the cerebral cortex during development. PMID: 22018691
    6. Gephyrin-mediated gamma-aminobutyric acid type A and glycine receptor clustering relies on a common binding site PMID: 22006921
    7. Activation and desensitization induce distinct conformational changes at the extracellular-transmembrane domain interface of the glycine receptor PMID: 21917927
    8. The steady state behavior of the recombinant homomeric alpha 1 glycine receptor has been analyzed at a range of low agonist concentrations. PMID: 11981023
    9. We analyzed single-channel recordings of rat recombinant alpha1 glycine receptors by fitting different mechanisms simultaneously to sets of sequences of openings at four glycine concentrations; gating efficacy increased as more molecules bind the channel PMID: 14749434
    10. Putative activation mechanisms were fitted to single-channel recordings at 4 glycine concentrations from rat alpha1beta receptors expressed in HEK293 cells. A substantial conformation change is caused by binding while the channel is still shut. PMID: 15574743
    11. molecular cloning; when present with other isoforms, GlyRalpha1del might alter the response to glycine or to other agonists, as this variant expands the potential heterogeneity among glycine receptors PMID: 15629462
    12. Reduction in affinity for the flipped conformation accounts for the reduction in cooperativity seen in the mutant glycine receptor alpha1, and it accounts for the increased EC50 for responses to glycine that is seen in mutant receptors. PMID: 17331994

    显示更多

    收起更多

  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse. Perikaryon. Cell projection, dendrite. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Glycine receptor (TC 1.A.9.3) subfamily, GLRA1 sub-subfamily
  • 组织特异性：
    Detected in spinal cord (at protein level). Detected in spinal cord and brain stem.
  • 数据库链接：

    KEGG: rno:25674

    UniGene: Rn.10109