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  4. Recombinant Rat Gamma-aminobutyric acid receptor subunit beta-2 (Gabrb2), partial

Recombinant Rat Gamma-aminobutyric acid receptor subunit beta-2 (Gabrb2), partial

  • 中文名称：
    大鼠Gabrb2重组蛋白
  • 货号：
    CSB-YP009147RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Gabrb2重组蛋白
  • 货号：
    CSB-EP009147RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Gabrb2重组蛋白
  • 货号：
    CSB-EP009147RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Gabrb2重组蛋白
  • 货号：
    CSB-BP009147RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Gabrb2重组蛋白
  • 货号：
    CSB-MP009147RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gabrb2
  • Uniprot No.：
  • 别名：
    Gabrb2; Gabrb-2; Gamma-aminobutyric acid receptor subunit beta-2; GABA(A) receptor subunit beta-2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer. The alpha1/beta2/gamma2 receptor and the alpha2/beta2/gamma2 receptor exhibit synaptogenic activity. Functions also as histamine receptor and mediates cellular responses to histamine.
  • 基因功能参考文献：
    1. the subunit arrangement of alpha4beta2delta GABAA receptors is not strongly predefined but is mostly satisfying the 2:2:1 subunit stoichiometry for alpha4, beta2, and delta subunits and that several subunit arrangements result in receptors with similar functional properties tuned to physiological conditions. PMID: 27382064
    2. The data of this study reveal that GABAA receptor beta2 and beta3 decrease following 1 h of acute restraint stress and may play a critical role in stress-induced hyperalgesia. PMID: 24992900
    3. These studies identify a novel gephyrin-binding motif in GABA(A) R beta2 and beta3 large cytoplasmic loops. PMID: 23205938
    4. The interaction between midazolam and propofol is affected by receptor subtype, and protein kinase phosphorylation influences their interaction on the alpha(1)beta(2)gamma(2)L receptor. PMID: 21474657
    5. Ligand- and subunit-specific conformational changes in the ligand-binding domain and the TM2-TM3 linker of {alpha}1 {beta}2 {gamma}2 GABAA receptors. PMID: 20937799
    6. Results indicate that beta2 Loop 9 is involved in receptor activation by GABA, propofol, and pregnanolone and that beta2(Q185) participates in hydrophilic interactions that are important for stabilizing the closed state of the GABA(A) receptor. PMID: 20007704
    7. The magnitude of GABA(A) receptor beta 2 subunit mRNA expression in rat telencephalic neuronal populations is found to be inferior to that of the distantly related hedgehog. PMID: 11845246
    8. TM1 glycine on the beta2 subunit is involved with desensitization of GABA-,pentobarbital-, and propofol-induced currents PMID: 11877425
    9. analysis of the role of a single M1 residue in the beta2 subunit of GABAA receptor alters channel gating of GABAA receptor in anesthetic modulation and direct activation PMID: 12939268
    10. identified a number of highly conserved charged residues in the GABAA-R beta2 subunit that appear to be involved in receptor activation PMID: 14610076
    11. GABA(B2) subunit mRNAs were were first weakly detected on GD 13.5. On GD 15.5, 17.5, and 19.5, these subunit mRNAs were found in the retina, hippocampus, cerebral cortex, spinal cord, and cerebellum area. PMID: 15158077
    12. Slow GABA-A receptor kinetics are not governed by simple ligand-receptor interactions. Access to the steroid direct gating (and perhaps potentiation) site occurs via the plasma membrane & the membrane concentration of steroid governs direct gating. PMID: 15282269
    13. To elucidate the role of the beta2Ile154-Asp163 region in GABA(A)R activation, each residue was individually mutated to cysteine and coexpressed with wild-type alpha1 subunits in Xenopus laevis oocytes. PMID: 15601928
    14. A gate exists between the picrotoxin binding site and the channel's extracellular end, consistent with a closed channel gate in the middle of the channel. PMID: 17227918
    15. Tonic activation of GABA-A receptors in developing hippocampus enhances glutamergic cell excitability and synaptic activity. PMID: 17317750
    16. alpha6-beta2-delta GABA A receptors exist in high/low affinity states and expression of subunits alters equilibrium between the two states. PMID: 17395622
    17. Using wild type/mutated receptor subunits to identify compounds with anesthetic effect. PMID: 18292428
    18. Gamma-aminobutyric acid (GABA) and pentobarbital induce different conformational rearrangements in the GABA A receptor alpha1 and beta2 pre-M1 regions. PMID: 18387955
    19. Intriguingly, although there is no direct interaction between GISP and the GABA(B2) subunit, their co-expression in HEK293 cells increases levels of GABA(B2). PMID: 18643869

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily, GABRB2 sub-subfamily
  • 组织特异性：
    Expressed in brain (at protein level).
  • 数据库链接：

    KEGG: rno:25451

    STRING: 10116.ENSRNOP00000004970

    UniGene: Rn.207158