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  4. Recombinant Rat Gamma-aminobutyric acid receptor subunit alpha-5 (Gabra5), partial

Recombinant Rat Gamma-aminobutyric acid receptor subunit alpha-5 (Gabra5), partial

  • 货号：
    CSB-YP009144RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009144RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009144RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009144RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009144RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gabra5
  • Uniprot No.：
  • 别名：
    Gabra5; Gabra-5; Gamma-aminobutyric acid receptor subunit alpha-5; GABA(A) receptor subunit alpha-5
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapse.
  • 基因功能参考文献：
    1. decreases in the alpha5 subunit of the GABAA receptor may be important in mediating the sympatho-excitation observed in both hypertension and pregnancy. PMID: 27038517
    2. Single unilateral infusion of NMDA at mild dose into the hippocampal formation induced chronic, focal neurodegeneration accompanied by severe and progressive neuroinflammation and affected preferentially principal neurons; GluN1 and GluN2B subunits of NMDA receptor were significantly upregulated up to 3 days after the NMDA infusion, while GABAA alpha5 subunit was downregulated at 30 days after the lesion PMID: 26930443
    3. In a chronic pain model, alpha5-GABAA receptors play a role in the loss of GABAergic inhibition and contribute to secondary allodynia. PMID: 26545088
    4. Report benzodiazepine derivative as GABA-site inhibitor of extra-synaptic GABAA alpha5 receptors. PMID: 26169564
    5. Study found that a significant portion of GABAergic postsynaptic compartments contain the alpha5 GABAAR subunit, both in vitro and in vivo PMID: 25663431
    6. Study showed: CA1 synapses display greater efficacy in ventral (VH) than dorsal (DH) poles; 10 Hz stimulation induces LTP less reliably in VH than in DH; and dorsal-ventral difference in LTP was eliminated after blockade of Gabra5s PMID: 24823837
    7. alpha2 GABAA receptor sub-units in the ventral hippocampus mediate unconditioned fear or anxiety, while alpha5 GABAA receptor sub-units in the dorsal hippocampus mediate conditioned fear memory. PMID: 23962649
    8. Methylation at one CpG island in Gabra5 promoter correlated with learning in memory impaired aged rats. PMID: 22869088
    9. Hippocampal expression of alpha(5) subunit decreases markedly in insular epilepsy. PMID: 22944187
    10. Chloride channel-2 is upregulated pyramidal cells of pilocarpine-treated rats; this involves alpha5 subunit-containing GABA(A) receptor. PMID: 21549811
    11. Our results demonstrated an early induction of the alpha 5 subunit expression (at mRNA and protein levels) during the first postnatal week, peaking at P5 and decreasing after this age. PMID: 15246695
    12. The results suggest that the synaptic alpha5-GABA(A)Rs might play a role in the phasic GABAergic inhibition of pyramidal neurons in hippocampus and cerebral cortex. PMID: 16998906

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily, GABRA5 sub-subfamily
  • 数据库链接：

    KEGG: rno:29707

    STRING: 10116.ENSRNOP00000014573

    UniGene: Rn.10368