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  4. Recombinant Rat Excitatory amino acid transporter 4 (Slc1a6), partial

Recombinant Rat Excitatory amino acid transporter 4 (Slc1a6), partial

  • 货号：
    CSB-YP021437RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021437RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021437RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021437RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021437RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Slc1a6; Eaat4Excitatory amino acid transporter 4; High-affinity neuronal glutamate transporter; Sodium-dependent glutamate/aspartate transporter; Solute carrier family 1 member 6
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate.
  • 基因功能参考文献：
    1. strate-dependent gating of anion channels associated with excitatory amino acid transporter 4. PMID: 21572047
    2. Data demonstrate that the neuronal glutamate transporter EAAT4 in Purkinje cells plays a critical role in the extrasynaptic diffusion of climbing fiber transmitters. PMID: 21070388
    3. The Na+ coupled glutamate carrier EAAT4 is stimulated by PIKfyve, which thus mimics the stimulating effect of the serum and glucocorticoid inducible kinase SGK1. PMID: 20110679
    4. The aspartate/glutamate transporter is localized in astrocytic processes around axonal or dendritic elements of glutamatergic axo-dendritic synaptic junctions in the frontoparietal somatosensory cortex. PMID: 11950769
    5. EAAT4 but not EAAT2 anion channels displays voltage-dependent gating modified by glutamate PMID: 14506254
    6. EAAT4 is expressed by astrocytes within the rat retina. PMID: 14727177
    7. These data suggest that PKC, possibly isozymes other than conventional ones, modulates the substrate-gated Cl(-) currents via rat EAAT4. PMID: 16601148
    8. glutamate carriers cooperatively interact during anion channel activation PMID: 16837599
    9. Glutamate transporter EAAT4 is increased in hippocampal astrocytes following traumatic Brain Injury in rats. PMID: 17490622
    10. Ethanol dose- and time-dependently reduced EAAT4 activity. PMID: 18540911
    11. detailed expression of EAAT4 protein in different adult rat fore- and midbrain regions was examined PMID: 18770868

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A6 subfamily
  • 数据库链接：

    KEGG: rno:84012

    STRING: 10116.ENSRNOP00000009931

    UniGene: Rn.10827