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  4. Recombinant Rat Excitatory amino acid transporter 3 (Slc1a1), partial

Recombinant Rat Excitatory amino acid transporter 3 (Slc1a1), partial

  • 货号：
    CSB-YP021432RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021432RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021432RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021432RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021432RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Slc1a1; Eaac1; Eaat3Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport. Negatively regulated by ARL6IP5.
  • 基因功能参考文献：
    1. miR-101b targets the neuronal glutamate transporter SLC1A1. Both mRNA and protein levels of SLC1A1 were found to be upregulated in the prefrontal cortex of rats genetically predisposed to develop major depressive disorder. PMID: 27507301
    2. Numb is a pivotal adaptor protein that mediates the subcellular localization of EAAT3 through binding the YxNxxF (where x stands for any amino acid) motif. PMID: 27358480
    3. Exercise-Mediated Increase in Nigral EAAC1 in aging rats. PMID: 26599339
    4. results suggest that the down-regulation of morphine-dependent EAAT3 expression in primary hippocampal cultures may be mediated by micro opioid receptor and delta opioid receptor and that kappa opioid receptor may not contribute significantly to this effect PMID: 25839761
    5. Chronic postnatal stress led to decreased Eaat3 expression in hippocampus and frontal cortex. PMID: 26037264
    6. data indicate that NRG1 induced the up-regulation of EAAC1 in primary cortical neurons with an increase in glutamate uptake PMID: 26092725
    7. changes of phosphorylated GluR1 subunit of AMPAR protein level in the hippocampus and frontal cortex, as well as markedly decreased EAAT2 mRNA expression in the hippocampus, striatum and frontal cortex and EAAT3 mRNA expression in the hippocampus PMID: 23694703
    8. Nicotine decreases EAAT3 activity, and this inhibition seems to be dependent on PKC and PI3K. PMID: 24355585
    9. Buffering and reuptake, but not presynaptic release, of glutamate is altered via Eaat3 in glutamate synapses in temporal lobe haloperidol-treated rat model of schizophrenia. PMID: 23356950
    10. Spinal EAAC1 expression is significantly increased only in the nonpainful groups on day 7 following whiplash injury. PMID: 22578356
    11. Increased EAAT3 expression in the hippocampus may be a compensatory response to possible deficits of glutamatergic neurotransmission induced by methamphetamine. PMID: 22540959
    12. interaction of NCX1 and EAAC1 transporters leads to glutamate-enhanced ATP production in brain mitochondria PMID: 22479505
    13. EAAC1 mRNA localizes to dendrites via dendritic targeting of EAAC1 mRNA, which is increased by seizure activity and may be regulated by neuronal activity/depolarization. PMID: 21185901
    14. Following subarachnoid hemorrhage there is significant downregulation of EAAC1 glutamate transporter in cerebral cortex and hippocampus. PMID: 21233495
    15. EAAT3 and EAAT4, but neither EAAT1 and EAAT2, nor EAAT2 and EAAT3 are capable of co-assembling into heterotrimers. Co-expression of EAAT3 and EAAT4 results in additional appearance of basolateral EAAT3 PMID: 21127051
    16. Existence of a pool of EAAC1 transporters associated with the actin binding protein alpha-adducin in a PKC-insensitive manner is suggested. PMID: 20493242
    17. Results indicate a transient decrease in EAAC1 glutamate transporter RNA expression in the rat hippocampus during the early stages of a spatial learning task. PMID: 20521381
    18. analysis of cation binding to the glutamate transporter EAAC1 probed with mutation of the conserved amino acid residue Thr10 PMID: 20378543
    19. Our findings reveal an adaptive response of subcortical white matter to increased levels of glutamate (by enhanced expression of EAAT1, 2 and 3) during focal cerebral ischemia which may limit excitotoxic damage. PMID: 19804828
    20. Our results suggest a critical role of serine 465 in the increased EAAT3 activity by protein kinase C activation PMID: 19922365
    21. An acute regulation of EAAC1 trafficking after activation of a G protein-coupled receptor. PMID: 12123836
    22. These results represent the first analysis of the synthesis and degradation of the EAAC1 amino acid transporter. PMID: 12151387
    23. Regulation of the neuronal glutamate transporter excitatory amino acid carrier-1 (EAAC1) by different protein kinase C subtypes. PMID: 12237337
    24. EAAC1 doesn't mediate GABA synthesis. By virtue of its location vis-a-vis synapses, EAAC1 may regulate glutamate uptake differentially. PMID: 12242481
    25. the glutamate binding site in EAAC1 is investigated PMID: 12419818
    26. EAAT3 protein expression in cultured cerebellar granule cells is not down-regulated when exposed to ammonia PMID: 12620282
    27. In C6 glioma cells, activation of PKC with phorbol 12-myristate 13-acetate (PMA) induced formation of EAAC1-PKCalpha complexes but did not induce formation of complexes with PKCdelta, a PKC not thought to regulate EAAC1 PMID: 12843260
    28. results suggest that the transient EAAC1 internalization participates in the modulation of the transporter function preventing excessive glutamate uptake to pyramidal neurons during the early stage of epilepsy PMID: 12875997
    29. EAAT3 mRNA expression was reduced ( from -49% to -28%) in the ileum and duodenum of Cryptosporidum parvum infected suckling rats PMID: 12923633
    30. EAAC1 trafficking is regulated by protein kinase C and platelet-derived growth factor PMID: 15197183
    31. Replacement of histidine-295 with glutamine, an amino acid residue that cannot be protonated, generates a fully functional EAAC1 transporter with transport kinetics that are close to those of the wild-type EAAC1. PMID: 15736956
    32. Together, the data presented in the current study suggest that ET-1 participates in the acute regulation of glutamate transport in glioma cells. PMID: 15737330
    33. These results suggest that EAAT3 regulates the amplitude of N-methyl-D-aspartate receptor currents at pre-saturated concentrations of glutamate to EAAT3. PMID: 15914650
    34. it is proposed that EAAC1 assembles as trimers of identical subunits but that the individual subunits in the trimer function independently of each other PMID: 16128593
    35. an EAAC1 carboxyl-terminal determinant is required for platelet-derived growth factor-dependent trafficking PMID: 16368696
    36. These results suggest that EAAT3 trafficking in C6 cells involves different pools of transporters. PMID: 16417946
    37. Ischemic preconditioning increases the expression of EAAT2 and EAAT3 glutamate transporters the upregulation of the latter being at least partly mediated by TNF-alpha converting enzyme/TNF-alpha/TNFR1 pathway. PMID: 16442237
    38. Asp-367, but not Asp-454, is involved in coordinating the bound Na(+) in the glutamate-free transporter form of the glutamate transporter EAAC1 PMID: 16478724
    39. SNAP-23 is required for constitutive recycling of EAAC1 PMID: 16516346
    40. overexpression of EAAC1 occurred mainly in structures prone to develop degenerating neurons PMID: 16538232
    41. EAAC1 is the high-affinity L-aspartate transporter for aspartate uptake & accumulation in prostate cells. Regulation of EAAC1 expression & L-aspartate transport by testosterone & prolactin is consistent with their regulation of citrate production. PMID: 16566829
    42. RFX1 enhances the activity of EAAT3 promoter to increase the expression of EAAT3 proteins PMID: 16723357
    43. Functions in rescuing PC12 cells and motor neurons from nerve growth factor deprivation and nerve injury, respectively. PMID: 16858406
    44. These findings provide new evidence that syntaxin 1A serves as an intrinsic enhancer to EAAC1 endocytic sorting and further suggest that syntaxin 1A is conversant with both ;ins' and ;outs' of synaptic neurotransmission. PMID: 16959903
    45. the bound substrate controls the rate and the extent of Na(+) interaction with the transporter, depending on the amino acid side chain in position 439 PMID: 17389249
    46. These studies suggest that NMDA receptors interact with excitatory amino acid carrier 1 (EAAC1), facilitate cell surface expression of EAAC1 under basal conditions, and control internalization of EAAC1 upon activation. PMID: 17459877
    47. environmental enrichment modulates these parts of the glutamatergic system PMID: 17854777
    48. Taken together, downregulation of glutamate transporters after ME is associated with dysregulation of basal glutamate concentrations and KCl-induced glutamate release in the brain. PMID: 18079058
    49. RTN2B functions as a positive regulator in the delivery of EAAC1 from the ER to the cell surface. PMID: 18096700
    50. different regulatory pathways of EAAC1 are associated with different neuronal maturation stages PMID: 18400334

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. Early endosome membrane. Late endosome membrane. Recycling endosome membrane.
  • 蛋白家族：
    Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A1 subfamily
  • 组织特异性：
    Expressed to a high extent in brain and kidney, and to a lower extent in heart, lung and skeletal muscle.
  • 数据库链接：

    KEGG: rno:25550

    STRING: 10116.ENSRNOP00000020272

    UniGene: Rn.6384