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  4. Recombinant Rat Eukaryotic translation initiation factor 4E (Eif4e)

Recombinant Rat Eukaryotic translation initiation factor 4E (Eif4e)

  • 货号：
    CSB-YP007556RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007556RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007556RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007556RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007556RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Eif4e; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-217
  • 氨基酸序列
    ATVEPETTP TTNPPPAEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENRDAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis. In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development.
  • 基因功能参考文献：
    1. Cerebral ischemia and subsequent reperfusion induced dephosphorylation and phosphorylation of 4E-BP2 at Thr37/Thr46. The induced phosphorylation coincided with the release of 4E-BP2 from eIF4E, although two phosphorylated 4E-BP2 forms were bound to eIF4E. PMID: 25822952
    2. Expression of eIF4E in hippocampus is higher in vascular dementia treated with electroacupuncture compared to untreated. PMID: 23053837
    3. The experiments reported herein examined the potential role for a rapamycin-sensitive signaling pathway in increasing formation of active eIF4F complex during meal feeding. PMID: 17634255
    4. the eIF4E-eIF4G complex is more stringently required for plasticity induced by initial learning than for that triggered by reactivation of an existing memory. PMID: 21289279
    5. Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E. PMID: 12080086
    6. Protein rescues cells from Myc-dependent apoptosis by inhibiting cytochrome c release. PMID: 12441348
    7. eIF4E phosphorylation has a role in preventing translation inhibition in a neuronal TGF-alpha pathway PMID: 12771152
    8. VEGF stimulates Erk-dependent phosphorylation of eIF4E, a crucial events in the initiation phase of protein translation. PMID: 15919658
    9. Acute heart ischemia downregulates cap-dependent translation through eIF4E sequestration triggered by eIF4G depletion. PMID: 16439989
    10. Leu stimulates a kinase B-independent signal pathway elevating the eIF4G-eIF4E complex assembly PMID: 17709445
    11. Blocking eukaryotic initiation factor 4F complex formation does not inhibit the mTORC1-dependent activation of protein synthesis in cardiomyocytes. PMID: 19074679

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  • 亚细胞定位：
    Cytoplasm, P-body. Cytoplasm. Cytoplasm, Stress granule. Nucleus.
  • 蛋白家族：
    Eukaryotic initiation factor 4E family
  • 组织特异性：
    Very high levels in post-meiotic testicular germ cells of rats of reproductive age.
  • 数据库链接：

    KEGG: rno:117045

    STRING: 10116.ENSRNOP00000063699

    UniGene: Rn.11275