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  4. Recombinant Rat Estrogen receptor (Esr1)

Recombinant Rat Estrogen receptor (Esr1)

  • 货号：
    CSB-YP007830RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007830RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007830RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007830RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007830RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Esr1; Esr; Estr; Nr3a1; Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-600
  • 氨基酸序列
    MTMTLHTKAS GMALLHQIQG NELEPLNRPQ LKMPMERALG EVYVDNSKPA VFNYPEGAAY EFNAAAAAAA AGASAPVYGQ SSITYGPGSE AAAFGANSLG AFPQLNSVSP SPLMLLHPPP HVSPFLHPHG HQVPYYLENE PSAYAVRDTG PPAFYRSNSD NRRQNGRERL SSSSEKGNMI MESAKETRYC AVCNDYASGY HYGVWSCEGC KAFFKRSIQG HNDYMCPATN QCTIDKNRRK SCQACRLRKC YEVGMMKGGI RKDRRGGRML KHKRQRDDLE GRNEMGTSGD MRAANLWPSP LVIKHTKKNS PALSLTADQM VSALLDAEPP LIYSEYDPSR PFSEASMMGL LTNLADRELV HMINWAKRVP GFGDLNLHDQ VHLLECAWLE ILMIGLVWRS MEHPGKLLFA PNLLLDRNQG KCVEGMVEIF DMLLATSSRF RMMNLQGEEF VCLKSIILLN SGVYTFLSST LKSLEEKDHI HRVLDKINDT LIHLMAKAGL TLQQQHRRLA QLLLILSHIR HMSNKGMEHL YNMKCKNVVP LYDLLLEMLD AHRLHAPASR MGVPPEEPSQ SQLTTTSSTS AHSLQTYYIP PEAEGFPNTI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3.
  • 基因功能参考文献：
    1. A trisubstituted pyrazole derivative reduces DMBA-induced mammary tumor growth in rats by inhibiting estrogen receptor-alpha expression. PMID: 29777335
    2. ERalpha has a profound regulatory role in ryanodine receptor-dependent transition to chronic pain PMID: 27499186
    3. MTA1 and MTA2 may be involved in the regulation of ERalpha transactivation activity in the epididymis of rats to facilitate a stable environment in the lumen PMID: 28789814
    4. In senile rats, expression of aromatase and ERalpha were increased in prostate epithelial cells. PMID: 29121167
    5. ERalpha overexpression was associated with an induction of its downstream genes, MUC-1 and IGF-1, suggesting that endosulfan might alter the uterine estrogenic pathway compromising uterine receptivity. PMID: 28559116
    6. examined the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ERbeta on freshly isolated peritoneal macrophages were examined. PMID: 28353029
    7. Study provide evidence that previous midlife oestradiol treatment maintains hippocampal oestrogen receptor alpha by decreasing its interaction with C-terminus of Hsc-70 interacting protein and that activation of these receptors provides cognitive benefits in the absence of circulating oestrogens. PMID: 27603028
    8. This comparative study documented the complexity of ERalpha genes, mRNAs, and proteins, and further determined the underlying structural basis of ligand-independent activation by C-terminally-truncated ERalpha variants. PMID: 28412386
    9. ESR1 is mainly involved with regulation of spermiogenesis, while ESR2 regulates spermatocyte apoptosis and spermiation. PMID: 27004961
    10. Both forms of estrogen receptors are involved in the short-latency facilitation of lordosis behavior in estradiol-primed rats. PMID: 27594441
    11. Alpha ESR down regulation is in involved in vascular remodeling in rats. PMID: 27698063
    12. ERalpha and GPER1 are localized exclusively to extranuclear sites in the striatum, and approximately 35% of the dendrites and axon terminals labeled for these receptors contain GABA immunoreactivity. PMID: 27080432
    13. This study evaluated the metabolism of progesterone receptors and estrogen receptor alpha in the ventrolateral division of the ventromedial hypothalamic nucleus. PMID: 28283583
    14. Mechanobiological effects of hydrostatic pressure are closely associated with ERalpha activity. MAPK molecules and F-actin were likely to be important mediator molecules in the ER-mediated mechanotransduction of BMSCs. PMID: 27256361
    15. Induction of apoptosis and downregulation of ERalpha in DMBA-induced mammary gland tumors in Sprague-Dawley rats by synthetic 3,5-disubstituted isoxazole derivatives. PMID: 27473146
    16. Study supports that regulatory estradiol action at the activity and protein abundance of ecto-5'-nucleotidase is mediated by estrogen ERalpha and ERbeta in the hippocampal synaptosomes PMID: 26987957
    17. Diet is a powerful modulator of ERalpha expression in adipose tissue, as nutritional modulation after an high-fat, high-sucrose diet strongly affects ERalpha expression, particularly in perirenal and epididymal adipose tissue. However, ERalpha expression in adipose tissue does not appear to be associated with whole-body glucose tolerance. PMID: 26923515
    18. Estrogen receptor alpha role in estradiol-17beta-D-glucuronide-induced impairment of Abcc2 function in hepatocytes PMID: 25813982
    19. Females with fewer ERalpha in the hypothalamic Ventromedial nucleus or Preoptic Area showed lower levels of both lordosis responses and paracopulatory behaviours compared to control females. PMID: 26314929
    20. Results suggest that E2 regulates MHC expression mainly through an ERalpha-mediated pathway with opposing effects on MHC-I and MHC-IIb. PMID: 24643056
    21. -protein-coupled receptor 30 (GPR30) and estrogen receptor alpha (ERalpha) is required for the action of peptide ERalpha17p activation of ERK1/2 (extracellular-signal-regulated kinase 1/2). PMID: 26371374
    22. MTA1 is coexpressed with ERa and ERb in the nuclei and cytoplasm of all placental cell types. PMID: 25217305
    23. During pregnancy, an increase in ERalpha expression in endothelial and vascular smooth muscle of the aorta and mesenteric artery is associated with increased ERalpha-mediated relaxation. PMID: 26408543
    24. ESR1 mediates the estradiol-induced activation of rapid signaling pathways in the epididymis. PMID: 26069273
    25. the influence of 17 beta-estradiol was lost in cirrhotic females, which may be attributed, at least partly, to intrahepatic ER alpha down-regulation in females with cirrhosis. PMID: 25864222
    26. These findings suggested that NG-R1 reduced endotoxin-induced cardiomyocyte apoptosis and the inflammatory response via the activation of ERalpha PMID: 25738436
    27. Data show a predominant localization of estrogen receptor alpha isoform ERalpha46 proteins in the nuclei. PMID: 26151894
    28. the deficiency during pregnancy and lactation impairs the expression of synapsins through a deregulation of ER-alpha pathway. PMID: 26018677
    29. Suggest ERalpha in mediates the inhibitory effect of estradiol on AQP2 expression in collecting ducts. PMID: 26062878
    30. Chronic treatment with CE increases ER-b but not ER-b expression in cerebral microvessels of OVX rats. Aging appears to reduce the normal ability of estrogen to increase ER-a expression in postischemic cerebral microvessels PMID: 25010766
    31. Study found novel splice variants of rat ERalpha that have alternative exons inserted in the ligand binding/AF-2 domain of the receptor. PMID: 25451981
    32. Neonatal testosterone acts via ESR1 to suppress central pattern generators required for pulsatile oxytocin release. PMID: 24518793
    33. Data suggest that oestradiol improves ventricular remodelling by both G-protein coupled oestrogen receptor (GPER)- and membrane-bound oestrogen receptor-alpha-dependent mechanism. PMID: 25256868
    34. Data suggest both Esr1/Esr2 play roles in testis in spermatogenesis/maintenance of fertility; Esr1 is mainly involved in negative feedback regulation of gonadotropins; both Esr1/Esr2 are involved in regulation of prolactin/testosterone production. PMID: 25869617
    35. brief period of mechanical loading significantly affects estrogen receptor-alpha in cancellous bone osteoblasts and osteocytes PMID: 25213639
    36. individual and simultaneous activation of ERalpha and ERbeta have different effects on the levels and subcellular location of PRs in VMNvl neurons PMID: 25612677
    37. Nuclear ERalpha is the primary mediator of E2's action on apo A-IV gene expression. PMID: 25051443
    38. This is the first time that GPER1 has been localized to the medial prefrontal cortex(mPFC) of rats and the first time that ERalpha and ERbeta have been described at extranuclear sites in the rat mPFC. PMID: 25211590
    39. findings also suggest the existence of new neuroendocrine control system between the right and left sides for the expression of ERalpha in the vlVMN PMID: 24629021
    40. Delayed healing rate and impaired callus quality in ovariectomy-induced osteoporotic fracture is related to the delayed expression of ERs. A high ER-alpha:ER-beta ratio favors callus formation. PMID: 25085540
    41. The aim of the present study was to investigate the role of each estrogen receptors on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats. PMID: 24056172
    42. Data indicate that compounds lacking the B ring of 17-hydroxy-beta-estradiol (E2) (A-CD compounds) showed differing ratios of estrogen receptors ERalpha:ERbeta binding affinities in the ovariectomized female rat. PMID: 24823389
    43. Membrane ERalpha attenuates myocardial fibrosis via RhoA/ROCK-mediated actin remodeling in ovariectomized female infarcted rats. PMID: 24292258
    44. Lycopene, a potent antioxidant, preserved the epididymis from PCB-induced damage and prevented changes in ESR1 levels. PMID: 23749762
    45. Data suggest that Esr1 knockout rats (here, generated from Holtzman Sprague-Dawley rats) exhibit both male and female infertility; these rats exhibit delta-482 Esr1 mutation due to premature stop codon. PMID: 24506075
    46. The ability of estrogen to augment resting type I and type II muscle fibre Hsp70 content is primarily mediated via muscle ERalpha. PMID: 24303535
    47. Sensitivity of hypothalamic ER alpha to negative energy balance may not be established in the early neonatal period, and that it develops by postnatal day 15. PMID: 24378445
    48. These results provide evidence for rapidly induced neuroprotection mediated by resveratrol activation of either estrogen receptor subtype within the ischemic cortex of rats. PMID: 23748073
    49. This study demonistrated that the estrogen receptor alpha protein in glutamatergic (vesicular glutamate transporter 2 immunoreactive) neurons of the female rat hypothalamus and amygdala. PMID: 23543101
    50. Following experimental disordered occlusion, gene expression of estrogen receptor alpha decreases in both male and female animals. PMID: 23020785

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Golgi apparatus. Cell membrane.
  • 蛋白家族：
    Nuclear hormone receptor family, NR3 subfamily
  • 数据库链接：

    KEGG: rno:24890

    STRING: 10116.ENSRNOP00000026350

    UniGene: Rn.10595